cDNA Clone FCHSD1

• Plasmid: pcDNA5-FRT/TO-FLAG FCHSD1
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG FCHSD1
• Genbank: NM_033449.1 GI:28875785*
• Tag: FLAG
• Species: Human

Sequence of Insert

AGATCTATGCAGCCGCCGCCCCGAAAAGTGAAGCCGGCCCAGGAGGTGAA
GCTTCGCTTCCTGGAACAGCTGAGCATCCTTCAGACCTGGCAGCAGAGGG
AGGCGGATCTGCTGGAGGACATCAGATCCTACAGCAAGCAGAGGGCAGCC
ATTGAACGGGAGTATGGGCAGGCACTCCAGAAACTGGCTGGCCCATTCCT
GAAGAGGGAAGGGCACCGGAGCGGTGAGATGGACAGCAGGACAGTGTTCG
GTGCCTGGCGCTGCCTGCTGGATGCCACCGTGGCTGGGGGCCAAACCCGA
CTCCAGGCGTCTGACCGATACCGTGACCTAGCAGGGGGTACAGGGCGGAG
CGCCAAGGAGCAGGTGCTTAGGAAGGGAACAGAGAACCTCCAGAGGGCGC
AGGCTGAGGTGCTGCAGTCTGTCCGGGAGCTGAGCCGAAGTCGGAAGCTG
TATGGGCAGCGGGAACGTGTGTGGGCCTTGGCACAGGAGAAGGCGGCTGA
TGTCCAGGCCAGGCTAAACCGAAGTGACCATGGGATCTTCCACTCTCGGA
CCAGTCTCCAGAAACTGAGCACCAAGCTGTCCGCCCAGTCAGCCCAGTAC
TCCCAGCAGCTGCAAGCAGCCCGCAATGAGTACCTGCTTAACTTGGTGGC
TACCAATGCCCACCTCGACCATTACTACCAGGAGGAACTGCCAGCTCTGC
TCAAGGCCCTGGTCAGTGAGCTGTCAGAGCACTTGAGGGACCCCCTGACC
TCCCTGAGCCACACTGAGCTGGAAGCCGCAGAGGTCATCCTGGAGCATGC
CCACCGCGGGGAGCAGACAACCTCCCAGGTAAGCTGGGAGCAAGACCTGA
AGCTGTTTCTTCAGGAGCCTGGTGTATTTTCCCCCACCCCACCTCAGCAG
TTTCAGCCAGCAGGGACTGATCAGGTGTGTGTCCTGGAGTGGGGAGCAGA
AGGCGTGGCTGGCAAGAGTGGCCTGGAGAAAGAGGTTCAGCGCTTGACCA
GCCGAGCTGCCCGTGACTACAAGATCCAGAACCATGGGCATCGGGTACTG
CAACGACTGGAGCAGAGGCGGCAGCAGGCTTCAGAGCGGGAGGCTCCAAG
CATAGAACAGAGGTTACAGGAAGTGCGAGAGAGCATCCGCCGGGCACAGG
TGAGCCAGGTGAAGGGGGCTGCCCGGCTGGCCCTGCTGCAGGGGGCTGGC
TTAGATGTGGAGCGCTGGCTGAAGCCAGCCATGACCCAGGCCCAGGATGA
GGTGGAGCAGGAGCGGCGGCTCAGTGAGGCTCGGCTGTCCCAGAGGGACC
TCTCTCCAACCGCTGAGGATGCTGAGCTTTCTGACTTTGAGGAATGTGAG
GAGACGGGAGAGCTCTTTGAGGAGCCTGCCCCCCAAGCCCTGGCCACGAG
GGCCCTCCCCTGCCCTGCACACGTGGTATTTCGCTATCAGGCAGGGCGTG
AGGATGAGCTGACAATCACGGAGGGTGAGTGGCTGGAGGTCATAGAGGAG
GGAGATGCTGACGAATGGGTCAAGGCTCGGAACCAGCACGGCGAGGTAGG
CTTTGTCCCTGAGCGATATCTCAACTTCCCGGACCTCTCCCTCCCAGAGA
GCAGCCAAGACAGTGACAATCCCTGCGGGGCAGAGCCCACAGCATTCCTG
GCACAGGCCCTGTACAGCTACACCGGACAGAGTGCAGAGGAGCTGAGCTT
CCCTGAGGGGGCACTCATCCGTCTGCTGCCCCGGGCCCAAGATGGAGTAG
ATGACGGCTTCTGGAGGGGAGAATTTGGGGGCCGTGTTGGGGTCTTCCCC
TCCCTGCTGGTGGAAGAGCTGCTTGGCCCCCCAGGGCCACCTGAACTCTC
TGACCCTGAACAGATGCTGCCGTCCCCTTCTCCTCCCAGCTTCTCCCCAC
CTGCACCTACCTCTGTGTTGGATGGGCCCCCTGCACCTGTCCTGCCTGGG
GACAAAGCCCTGGACTTCCCTGGGTTCCTGGACATGATGGCACCTCGACT
CAGGCCGATGCGTCCACCACCTCCCCCGCCGGCTAAAGCCCCGGATCCTG
GCCACCCAGATCCCCTCACCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMQPPPRKVKPAQEVKLRFLEQLSILQTWQQREADLLEDI
RSYSKQRAAIEREYGQALQKLAGPFLKREGHRSGEMDSRTVFGAWRCLLD
ATVAGGQTRLQASDRYRDLAGGTGRSAKEQVLRKGTENLQRAQAEVLQSV
RELSRSRKLYGQRERVWALAQEKAADVQARLNRSDHGIFHSRTSLQKLST
KLSAQSAQYSQQLQAARNEYLLNLVATNAHLDHYYQEELPALLKALVSEL
SEHLRDPLTSLSHTELEAAEVILEHAHRGEQTTSQVSWEQDLKLFLQEPG
VFSPTPPQQFQPAGTDQVCVLEWGAEGVAGKSGLEKEVQRLTSRAARDYK
IQNHGHRVLQRLEQRRQQASEREAPSIEQRLQEVRESIRRAQVSQVKGAA
RLALLQGAGLDVERWLKPAMTQAQDEVEQERRLSEARLSQRDLSPTAEDA
ELSDFEECEETGELFEEPAPQALATRALPCPAHVVFRYQAGREDELTITE
GEWLEVIEEGDADEWVKARNQHGEVGFVPERYLNFPDLSLPESSQDSDNP
CGAEPTAFLAQALYSYTGQSAEELSFPEGALIRLLPRAQDGVDDGFWRGE
FGGRVGVFPSLLVEELLGPPGPPELSDPEQMLPSPSPPSFSPPAPTSVLD
GPPAPVLPGDKALDFPGFLDMMAPRLRPMRPPPPPPAKAPDPGHPDPLT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16676
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NM_033449.1 CDS:; delta gcaggg at nt 230-235 (delta R78 and G79) this deletion present in majority of ESTs