cDNA Clone LISCH

• Plasmid: pGEX6P-2-LISCH
• Parent Plasmid: pGEX6P2
• Expressed: GST LISCH
• Genbank: NM_205834.2 GI:47777760
• Tag: GST
• Species: Human

Sequence of Insert

ggatccatgcaacaggacggacttggagtagggacaaggaacggaagtgggaaggggaggagcgtgcacccctcctggccttggtgcgcgccgcgccccctaaggtactttggaagggacgcgcgggccagacgcgcccagacggccgcgatggcgctgttggccggcgggctctccagagggctgggctcccacccggccgccgcaggccgggacgcggtcgtcttcgtgtggcttctgcttagcacctggtgcacagctcctgccagggccatccaggtgaccgtgtccaacccctaccacgtg
gtgatcctcttccagcctgtgaccctgccctgtacctaccagatgacctc
gacccccacgcaacccatcgtcatctggaagtacaagtctttctgccggg
accgcatcgccgatgccttctccccggccagcgtcgacaaccagctcaat
gcccagctggcagccgggaacccaggctacaacccctacgttgagtgcca
ggacagcgtgcgcaccgtcagggtcgtggccaccaagcagggcaacgctg
tgaccctgggagattactaccagggccggaggattaccatcaccggaaat
gctgacctgacctttgaccagacggcgtggggggacagtggtgtgtatta
ctgctccgtggtctcagcccaggacctccaggggaacaatgaggcctacg
cagagctcatcgtccttgggaggacctcaggggtggctgagctcttacct
ggttttcaggcggggcccatagaagactggctcttcgtggttgtggtatg
cctggctgccttcctcatcttcctcctcctgggcatctgctggtgccagt
gctgcccgcacacttgctgctgctacgtcaggtgcccctgctgcccagac
aagtgctgctgccccgaggccctgtatgccgccggcaaagcagccacctc
aggtgttcccagcatttatgcccccagcacctatgcccacctgtctcccg
ccaagaccccacccccaccagctatgattcccatgggccctgcctacaac
gggtaccctggaggataccctggagacgttgacaggagtagctcagctgg
tggccaaggctcctatgtacccctgcttcgggacacggacagcagtgtgg
cctctgaagtccgcagtggctacaggattcaggccagccagcaggacgac
tccatgcgggtcctgtactacatggagaaggagctggccaacttcgaccc
ttctcgacctggcccccccagtggccgtgtggagcgggccatgagtgaag
tcacctccctccacgaggacgactggcgatctcggccttcccggggccct
gccctcaccccgatccgggatgaggagtggggtggccactccccccggag
tcccaggggatgggaccaggagcccgccagggagcaggcaggcgggggct
ggcgggccaggcggccccgggcccgctccgtggacgccctggacgacctc
accccgccgagcaccgccgagtcagggagcaggtctcccacgagtaatgg
tgggagaagccgggcctacatgcccccgcggagccgcagccgggacgacc
tctatgaccaagacgactcgagggacttcccacgctcccgggacccccac
tacgacgacttcaggtctcgggagcgccctcctgccgaccccaggtccca
ccaccaccgtacccgggaccctcgggacaacggctccaggtccggggacc
tcccctatgatgggcggctactggaggaggctgtgaggaagaaggggtcg
gaggagaggaggagaccccacaaggaggaggaggaagaggcctactaccc
gcccgcgccgcccccgtactcggagaccgactcgcaggcgtcccgagagc
gcaggctcaagaagaacttggccctgagtcgggaaagtttagtcgtctga
gcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMQQDGLGVGTRNGSGKGRS
VHPSWPWCAPRPLRYFGRDARARRAQTAAMALLAGGLSRGLGSHPAAAGR
DAVVFVWLLLSTWCTAPARAIQVTVSNPYHVVILFQPVTLPCTYQMTSTP
TQPIVIWKYKSFCRDRIADAFSPASVDNQLNAQLAAGNPGYNPYVECQDS
VRTVRVVATKQGNAVTLGDYYQGRRITITGNADLTFDQTAWGDSGVYYCS
VVSAQDLQGNNEAYAELIVLGRTSGVAELLPGFQAGPIEDWLFVVVVCLA
AFLIFLLLGICWCQCCPHTCCCYVRCPCCPDKCCCPEALYAAGKAATSGV
PSIYAPSTYAHLSPAKTPPPPAMIPMGPAYNGYPGGYPGDVDRSSSAGGQ
GSYVPLLRDTDSSVASEVRSGYRIQASQQDDSMRVLYYMEKELANFDPSR
PGPPSGRVERAMSEVTSLHEDDWRSRPSRGPALTPIRDEEWGGHSPRSPR
GWDQEPAREQAGGGWRARRPRARSVDALDDLTPPSTAESGSRSPTSNGGR
SRAYMPPRSRSRDDLYDQDDSRDFPRSRDPHYDDFRSRERPPADPRSHHH
RTRDPRDNGSRSGDLPYDGRLLEEAVRKKGSEERRRPHKEEEEEAYYPPA
PPPYSETDSQASRERRLKKNLALSRESLVV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU16630
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 98258 Daltons