cDNA Clone LIS1

• Plasmid: pGEX6P-Lis1
• Parent Plasmid: pGEX6P1
• Expressed: GST-LIS1
• Genbank: NM_000430.2
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGGTGCTGTCCCAGAGACAACGAGATGAACTAAATCGAGCTAT
AGCAGATTATCTTCGTTCAAATGGCTATGAAGAGGCATATTCAGTTTTTA
AAAAGGAAGCTGAATTAGATGTGAATGAAGAATTAGATAAAAAGTATGCT
GGTCTTTTGGAAAAAAAATGGACATCTGTTATTAGATTACAAAAGAAGGT
TATGGAATTAGAATCAAAGCTAAATGAAGCAAAAGAAGAATTTACGTCAG
GTGGACCTCTTGGTCAGAAACGAGACCCAAAAGAATGGATTCCCCGTCCG
CCAGAAAAATATGCATTGAGTGGTCACAGGAGTCCAGTCACTCGAGTCAT
TTTCCATCCTGTGTTCAGTGTTATGGTCTCTGCTTCAGAGGATGCTACAA
TTAAGGTGTGGGATTATGAGACTGGAGATTTTGAACGAACTCTTAAAGGA
CATACAGACTCTGTACAGGACATTTCATTCGACCACAGCGGCAAGCTTCT
GGCTTCCTGTTCTGCAGATATGACCATTAAACTATGGGATTTTCAGGGCT
TTGAATGCATCAGAACCATGCACGGCCATGACCACAATGTTTCTTCAGTA
GCCATCATGCCCAATGGAGATCATATAGTGTCTGCCTCAAGGGATAAAAC
TATAAAAATGTGGGAAGTGCAAACTGGCTACTGTGTGAAGACATTCACAG
GACACAGAGAATGGGTACGTATGGTACGGCCAAATCAAGATGGCACTCTG
ATAGCCAGCTGTTCCAATGACCAGACTGTGCGTGTATGGGTCGTAGCAAC
AAAGGAATGCAAGGCTGAGCTCCGAGAGCATGAGCATGTGGTAGAATGCA
TTTCCTGGGCTCCAGAAAGCTCATATTCCTCCATCTCTGAAGCAACAGGA
TCTGAGACTAAAAAAAGTGGTAAACCTGGGCCATTCTTGCTGTCTGGATC
CAGAGACAAGACTATTAAGATGTGGGATGTCAGTACTGGCATGTGCCTTA
TGACCCTCGTGGGTCATGATAACTGGGTACGTGGAGTTCTGTTCCATTCT
GGGGGGAAGTTTATTTTGAGTTGTGCTGATGACAAGACCCTACGCGTATG
GGATTACAAGAACAAGCGATGCATGAAGACCCTCAATGCGCATGAACACT
TTGTTACCTCCTTGGATTTCCACAAGACGGCACCCTATGTCGTCACTGGC
AGCGTAGATCAAACAGTAAAAGTGTGGGAGTGCCGTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMVLSQRQRDELNRAIA
DYLRSNGYEEAYSVFKKEAELDVNEELDKKYAGLLEKKWTSVIRLQKKVM
ELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIF
HPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDHSGKLLA
SCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTI
KMWEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATK
ECKAELREHEHVVECISWAPESSYSSISEATGSETKKSGKPGPFLLSGSR
DKTIKMWDVSTGMCLMTLVGHDNWVRGVLFHSGGKFILSCADDKTLRVWD
YKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU16298
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 73833 Daltons