cDNA Clone PCF11

• Plasmid: pEFIRES-B-eYFP-PCF11 c1236/1242g t1239c
• Parent Plasmid: pEFIRES blasto eYFPC1
• Expressed: eYFP-PCF11 c1236/1242g t1239c
• Genbank: GENE 51585
• Tag: YFP
• Species: Human

Sequence of Insert

gtcgacgtATGTCAGAGCAGACGCCGGCCGAGGCCGGTGCTGCGGGGGCCCGGGAGGACGCCTGTCGGGATTATCAGTCATCGCTCGAAGACCTGACCTTCAATAGCAAGCCGCACATCAATATGCTGACCATTCTAGCCGAGGAGAACCTGCCCTTCGCCAAGGAGATCGTCTCTCTCATCGAGGCCCAAACCGCCAAGGCTCCTTCCTCAGAGAAGCTTCCTGTTATGTACCTTATGGATTCTATTGTGAAAAACGTTGGAAGAGAGTATCTCACTGCCTTTACTAAAAATCTAGTTGCAACATTTATTTGTGTGTTTGAAAAGGTGGATGAAAATACTAGGAAAAGTTTATTTAAGTTACGTTCTACATGGGATGAAATATTCCCTTTGAAGAAACTTTATGCCCTGGATGTCAGAGTCAATTCATTAGATCCTGCTTGGCCTATTAAACCTCTACCCCCCAATGTGAATACGTCTAGCATCCATGTGAATCCTAAATTTTTAAATAAATCGCCCGAGGAGCCTTCAACACCTGGTACAGTGGTCAGTTCCCCTAGCATCTCCACTCCTCCAATTGTTCCTGATATACAAAAGAATCTTACACAAGAACAACTAATAAGGCAGCAGTTACTGGCAAAGCAAAAACAGTTGTTAGAACTTCAGCAGAAAAAGCTGGAGCTTGAGCTAGAGCAAGCTAAGGCACAGTTGGCAGTTTCTCTTAGTGTTCAGCAGGAAACATCCAATTTAGGTCCTGGATCTGCACCATCCAAATTACATGTTTCACAGATTCCCCCTATGGCAGTTAAAGCTCCTCATCAGGTTCCTGTGCAATCTGAGAAAAGCCGTCCAGGACCATCCTTACAAATTCAGGATTTAAAAGGAACTAACCGGGATCCTCGTCTGAACAGGATAAGCCAACATTCTCATGGAAAAGATCAGAGTCACAGGAAAGAATTTCTAATGAACACATTAAACCAGTCTGATACTAAGACAAGTAAAACTATACCCTCTGAAAAACTAAATTCATCCAAGCAAGAAAAAAGTAAATCAGGTGAAAAAATAACCAAGAAAGAACTTGACCAATTAGATTCTAAATCGAAATCGAAATCGAAATCACCCTCACCTTTGAAAAACAAATTATCTCACACAAAAGACTTGAAAAATCAAGAATCGGAAAGTATGAGGTTGTCTGATATGAACAAGAGAGATCCAAGATTAAAAAAACATCTTCAGGATAAGACCGATGGCAAAGATGATGATGTGAAAGAGAAGAGAAAAACTGCAGAAAAAAAGGATAAAGATGAGCACATGAAGTCATCCGAACACAGACTGGCTGGAAGTAGAAATAAAATCATAAATGGCATTGTACAAAAACAGGATACAATAACAGAAGAGTCAGAAAAACAGGGGACAAAACCAGGGAGATCGAGTACTAGAAAGCGATCAAGATCTCGATCACCCAAGTCTAGGTCACCAATTATACATTCCCCAAAGAGAAGAGATAGGCGGTCACCCAAACGAAGGCAAAGAAGTATGTCTCCAACATCGACACCTAAAGCTGGAAAGATTCGCCAATCTGGAGCTAAGCAGTCACATATGGAAGAGTTTACACCACCTTCTAGGGAAGACAGAAATGCTAAGAGAAGTACTAAACAGGATATTCGGGATCCAAGGCGAATGAAAAAGACTGAAGAGGAGCGACCACAAGAAACTACAAATCAGCATTCTACAAAGTCAGGCACTGAACCAAAGGAGAATGTAGAAAACTGGCAAAGTTCCAAGTCTGCCAAAAGATGGAAATCTGGTTGGGAAGAAAATAAAAGCTTACAACAGGTTGATGAACATAGTAAACCTCCTCATCTGAGGCATAGGGAGAGCTGGTCAAGCACTAAAGGAATTTTATCACCTCGAGCCCCAAAGCAGCAACAGCATCGATTAAGTGTAGATGCCAATCTTCAGATTCCTAAAGAGTTAACTCTTGCAAGCAAAAGAGAATTACTTCAAAAGACGAGTGAACGTTTAGCATCTGGTGAAATTACACAGGATGACTTCCTTGTTGTTGTGCATCAAATTCGACAGCTATTTCAGTATCAAGAAGGTGTGCGAGAAGAGCAGAGATCTCCATTCAATGATCGTTTTCCACTTAAGCGACCTCGATATGAAGATTCAGATAAACCATTTGTAGATAGTCCAGCATCAAGATTCGCCGGCCTGGATACAAATCAGCGACTTACAGCTTTAGCTGAAGACAGACCGTTATTTGATGGACCTAGTAGGCCATCAGTAGCAAGAGATGGCCCAACGAAGATGATTTTTGAAGGACCCAATAAATTAAGCCCTCGAATTGATGGACCTCCCACACCAGCTTCTCTTCGGTTTGATGGGTCACCAGGACAAATGGGGGGAGGAGGCCCTTTGAGATTTGAGGGGCCACAAGGTCAGCTAGGAGGTGGGTGTCCTTTGAGATTTGAAGGTCCTCCAGGACCAGTGGGGACACCTCTGCGGTTTGAGGGCCCAATTGGTCAAGCAGGAGGAGGTGGTTTTCGGTTTGAAGGTTCCCCTGGTCTGAGGTTTGAGGGATCTCCAGGTGGTTTGAGATTTGAGGGACCAGGAGGCCAGCCTGTGGGTGGTCTGAGGTTTGAGGGACATCGTGGTCAACCTGTGGGTGGTCTAAGGTTTGAGGGACCTCATGGTCAGCCTGTGGGTGGACTTAGATTTGATAATCCCCGAGGTCAGCCTGTAGGTGGACTTAGATTTGAGGGGGGTCATGGTCCATCAGGGGCTGCGATTAGGTTTGATGGACCTCATGGTCAGCCAGGAGGTGGAATCAGATTTGAGGGCCCTTTGCTACAGCAAGGGGTTGGAATGAGGTTTGAGGGCCCCCATGGTCAGTCAGTAGCTGGTCTGAGATTTGAGGGACAACATAATCAACTTGGTGGGAACCTTAGGTTTGAGGGTCCACATGGTCAGCCAGGGGTTGGTATCAGGTTTGAAGGCCCTTTAGTCCAACAAGGAGGTGGAATGAGGTTTGAGGGTCCTTCTGTACCAGGAGGTGGCCTGAGAATTGAAGGGCCTCTGGGTCAAGGTGGTCCAAGATTTGAAGGTTGTCATGCTTTAAGGTTTGATGGGCAGCCAGGTCAGCCGTCACTCTTGCCAAGATTTGATGGATTACATGGTCAGCCAGGTCCTAGATTTGAAAGGACTCCTGGTCAGCCAGGCCCTCAGAGGTTTGATGGACCACCTGGACAGCAGGTTCAACCCAGATTTGACGGTGTACCTCAAAGATTTGATGGTCCACAACATCAGCAAGCATCAAGGTTTGATATTCCTCTTGGTCTTCAAGGCACAAGATTTGACAATCATCCTTCACAAAGGCTTGAATCAGTATCTTTCAATCAGACTGGTCCATATAATGATCCACCTGGCAATGCTTTTAATGCCCCATCCCAAGGACTACAGTTCCAAAGACATGAACAAATATTTGATTCACCTCAAGGACCAAATTTTAATGGACCACATGGCCCTGGAAACCAGAGTTTCTCTAATCCACTTAACAGAGCTTCTGGACACTATTTTGATGAAAAAAATCTTCAGAGTTCTCAATTTGGAAACTTTGGCAATATACCTGCTCCAATGACAGTAGGAAATATTCAGGCATCTCAACAGGTTCTGAGTGGTGTTGCTCAGCCAGTAGCTTTTGGTCAAGGACAACAGTTTTTACCAGTTCATCCACAAAATCCTGGATTTGTTCAGAATCCTTCAGGAGCCCTCCCTAAGGCATATCCTGATAATCATCTCAGTCAGGTGGATGTAAATGAATTGTTTTCAAAATTGCTAAAAACAGGAATTCTCAAATTGTCCCAAACTGATTCAGCTACAACACAAGTAAGTGAAGTAACTGCTCAGCCTCCCCCTGAAGAGGAGGAAGATCAAAATGAAGATCAAGATGTTCCAGATCTTACTAATTTTACAGTTGAAGAATTGAAACAACGTTATGACAGTGTTATAAATCGACTGTACACTGGTATTCAGTGTTACTCTTGTGGAATGAGGTTTACAACATCACAGACAGATGTTTATGCAGATCATTTGGACTGGCATTATCGGCAAAATAGAACTGAAAAGGATGTTAGCCGAAAAGTCACTCATAGACGTTGGTACTACAGTTTAACAGACTGGATAGAATTTGAGGAGATAGCTGATCTGGAAGAACGGGCAAAGAGCCAGTTTTTTGAAAAGGTGCATGAAGAAGTTGTGCTCAAAACTCAAGAGGCTGCTAAAGAAAAAGAGTTCCAAAGTGTACCTGCTGGACCAGCTGGAGCAGTTGAGAGTTGTGAAATCTGTCAAGAACAATTTGAACAATACTGGGATGAAGAAGAGGAGGAATGGCATTTGAAAAATGCTATTAGAGTAGATGGAAAGATTTATCATCCATCATGTTATGAAGATTATCAAAATACATCTTCATTTGATTGTACACCATCTCCCAGCAAGACACCAGTTGAAAACCCCTTGAATATTATGTTGAACATTGTCAAAAACGAATTGCAGGAACCCTGTGACAGTCCCAAAGTTAAGGAAGAACGAATTGATACACCACCAGCTTGTACAGAGGAAAGCATAGCAACACCCTCTGAAATTAAAACAGAAAATGACACAGTCGAGTCAGTTTAAgcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRRMSEQTPAEAGAAGAREDACRDYQSSLEDLTFNSKPHINMLTILAEENLPFAKEIVSLIEAQTAKAPSSEKLPVMYLMDSIVKNVGREYLTAFTKNLVATFICVFEKVDENTRKSLFKLRSTWDEIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEEPSTPGTVVSSPSISTPPIVPDIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQAKAQLAVSLSVQQETSNLGPGSAPSKLHVSQIPPMAVKAPHQVPVQSEKSRPGPSLQIQDLKGTNRDPRLNRISQHSHGKDQSHRKEFLMNTLNQSDTKTSKTIPSEKLNSSKQEKSKSGEKITKKELDQLDSKSKSKSKSPSPLKNKLSHTKDLKNQESESMRLSDMNKRDPRLKKHLQDKTDGKDDDVKEKRKTAEKKDKDEHMKSSEHRLAGSRNKIINGIVQKQDTITEESEKQGTKPGRSSTRKRSRSRSPKSRSPIIHSPKRRDRRSPKRRQRSMSPTSTPKAGKIRQSGAKQSHMEEFTPPSREDRNAKRSTKQDIRDPRRMKKTEEERPQETTNQHSTKSGTEPKENVENWQSSKSAKRWKSGWEENKSLQQVDEHSKPPHLRHRESWSSTKGILSPRAPKQQQHRLSVDANLQIPKELTLASKRELLQKTSERLASGEITQDDFLVVVHQIRQLFQYQEGVREEQRSPFNDRFPLKRPRYEDSDKPFVDSPASRFAGLDTNQRLTALAEDRPLFDGPSRPSVARDGPTKMIFEGPNKLSPRIDGPPTPASLRFDGSPGQMGGGGPLRFEGPQGQLGGGCPLRFEGPPGPVGTPLRFEGPIGQAGGGGFRFEGSPGLRFEGSPGGLRFEGPGGQPVGGLRFEGHRGQPVGGLRFEGPHGQPVGGLRFDNPRGQPVGGLRFEGGHGPSGAAIRFDGPHGQPGGGIRFEGPLLQQGVGMRFEGPHGQSVAGLRFEGQHNQLGGNLRFEGPHGQPGVGIRFEGPLVQQGGGMRFEGPSVPGGGLRIEGPLGQGGPRFEGCHALRFDGQPGQPSLLPRFDGLHGQPGPRFERTPGQPGPQRFDGPPGQQVQPRFDGVPQRFDGPQHQQASRFDIPLGLQGTRFDNHPSQRLESVSFNQTGPYNDPPGNAFNAPSQGLQFQRHEQIFDSPQGPNFNGPHGPGNQSFSNPLNRASGHYFDEKNLQSSQFGNFGNIPAPMTVGNIQASQQVLSGVAQPVAFGQGQQFLPVHPQNPGFVQNPSGALPKAYPDNHLSQVDVNELFSKLLKTGILKLSQTDSATTQVSEVTAQPPPEEEEDQNEDQDVPDLTNFTVEELKQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRTEKDVSRKVTHRRWYYSLTDWIEFEEIADLEERAKSQFFEKVHEEVVLKTQEAAKEKEFQSVPAGPAGAVESCEICQEQFEQYWDEEEEEWHLKNAIRVDGKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKNELQEPCDSPKVKEERIDTPPACTEESIATPSEIKTENDTVESV*

• Vector Type: Other
• DU Number: DU23036
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: carries triple mutation compared to ref sequence shown, also harbours c546t (silent at Ser 182)