cDNA Clone Triad1

• Synonyms: ARIH2
• Plasmid: pMal6P-1 Triad1-6His
• Parent Plasmid: pMAL6P
• Expressed: MBP-Triad1-6His
• Genbank: NM_006321
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGTCAGTGGACATGAATAGCCAGGGGTCTGACAGCAATGAAGA
GGACTATGACCCAAATTGTGAGGAAGAGGAAGAAGAAGAAGAAGACGACC
CTGGGGACATAGAGGACTATTACGTGGGAGTAGCCAGCGATGTGGAGCAG
CAGGGGGCTGATGCCTTTGATCCCGAGGAGTACCAGTTCACTTGCTTGAC
CTACAAGGAATCTGAGGGTGCCCTCAATGAGCACATGACCAGCTTAGCTT
CTGTCCTAAAGGTATCTCATTCAGTTGCTAAACTTATATTAGTTAATTTC
CACTGGCAAGTTTCAGAGATATTGGACAGATACAAGTCCAATTCTGCTCA
ACTGCTTGTTGAGGCTCGAGTTCAGCCTAATCCATCAAAACATGTTCCCA
CATCCCATCCCCCTCACCACTGTGCAGTGTGTATGCAGTTTGTGCGAAAG
GAAAACCTACTCTCTCTGGCCTGTCAGCACCAGTTTTGCCGCAGCTGCTG
GGAGCAGCACTGCTCAGTTCTCGTCAAGGACGGCGTGGGCGTGGGAGTCT
CTTGCATGGCTCAGGACTGTCCACTCCGTACACCAGAGGACTTTGTGTTT
CCATTGCTTCCCAATGAAGAATTGAGAGAGAAATACAGGCGCTACCTCTT
CAGGGACTATGTGGAGAGTCATTACCAGCTCCAGCTGTGCCCTGGTGCAG
ACTGCCCCATGGTTATTCGGGTACAGGAGCCTAGAGCTCGCCGAGTACAG
TGCAATCGGTGCAACGAGGTCTTCTGTTTCAAGTGTCGTCAGATGTATCA
CGCACCCACAGACTGTGCCACAATCCGGAAATGGCTCACGAAGTGTGCAG
ACGACTCTGAAACAGCCAACTACATTAGTGCTCACACTAAAGACTGTCCC
AAGTGCAACATCTGCATTGAGAAGAATGGAGGCTGCAATCACATGCAATG
CTCCAAATGTAAACACGACTTCTGCTGGATGTGTCTAGGAGATTGGAAGA
CTCATGGCAGTGAATACTATGAGTGCAGTCGTTACAAGGAGAATCCTGAC
ATCGTGAACCAGAGCCAACAAGCCCAGGCGAGGGAAGCCCTCAAGAAGTA
CTTATTCTACTTTGAGAGGTGGGAAAACCACAATAAAAGCTTGCAGCTAG
AGGCACAGACATACCAGCGGATTCACGAGAAGATTCAGGAGAGGGTCATG
AACAATCTGGGGACATGGATCGACTGGCAGTACCTACAGAATGCTGCCAA
GCTCTTGGCCAAGTGTCGATACACCCTGCAATACACCTACCCATATGCAT
ATTACATGGAGTCCGGACCCAGGAAGAAGCTGTTTGAATACCAGCAGGCT
CAGCTGGAGGCTGAGATCGAAAACCTCTCATGGAAAGTGGAGCGTGCAGA
CAGCTATGACAGAGGGGACTTGGAGAACCAGATGCATATAGCGGAGCAGC
GGAGGAGAACCCTGCTGAAAGATTTCCATGACACCCATCATCACCATCAC
CATTAAGCGGCCGC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSMSVDMNSQGSDSNEEDYDPNCE
EEEEEEEDDPGDIEDYYVGVASDVEQQGADAFDPEEYQFTCLTYKESEGA
LNEHMTSLASVLKVSHSVAKLILVNFHWQVSEILDRYKSNSAQLLVEARV
QPNPSKHVPTSHPPHHCAVCMQFVRKENLLSLACQHQFCRSCWEQHCSVL
VKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKYRRYLFRDYVESH
YQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCAT
IRKWLTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDF
CWMCLGDWKTHGSEYYECSRYKENPDIVNQSQQAQAREALKKYLFYFERW
ENHNKSLQLEAQTYQRIHEKIQERVMNNLGTWIDWQYLQNAAKLLAKCRY
TLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDL
ENQMHIAEQRRRTLLKDFHDTHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20861
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid to express Triad-6HIS with N-terminal MBP tag (contains preScission protease site)