cDNA Clone TARDBP43

• Synonyms: ALS10, TDP-43, TARDBP
• Plasmid: pcDNA5-FRT/TO-GFP-TARDBP43
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-TARDBP43
• Genbank: NM_007375.3
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCTCTGAATATATTCGGGTAACCGAAGATGAGAACGATGAGCCCAT
TGAAATACCATCGGAAGACGATGGGACGGTGCTGCTCTCCACGGTTACAG
CCCAGTTTCCAGGGGCGTGTGGGCTTCGCTACAGGAATCCAGTGTCTCAG
TGTATGAGAGGTGTCCGGCTGGTAGAAGGAATTCTGCATGCCCCAGATGC
TGGCTGGGGAAATCTGGTGTATGTTGTCAACTATCCAAAAGATAACAAAA
GAAAAATGGATGAGACAGATGCTTCATCAGCAGTGAAAGTGAAAAGAGCA
GTCCAGAAAACATCCGATTTAATAGTGTTGGGTCTCCCATGGAAAACAAC
CGAACAGGACCTGAAAGAGTATTTTAGTACCTTTGGAGAAGTTCTTATGG
TGCAGGTCAAGAAAGATCTTAAGACTGGTCATTCAAAGGGGTTTGGCTTT
GTTCGTTTTACGGAATATGAAACACAAGTGAAAGTAATGTCACAGCGACA
TATGATAGATGGACGATGGTGTGACTGCAAACTTCCTAATTCTAAGCAAA
GCCAAGATGAGCCTTTGAGAAGCAGAAAAGTGTTTGTGGGGCGCTGTACA
GAGGACATGACTGAGGATGAGCTGCGGGAGTTCTTCTCTCAGTACGGGGA
TGTGATGGATGTCTTCATCCCCAAGCCATTCAGGGCCTTTGCCTTTGTTA
CATTTGCAGATGATCAGATTGCGCAGTCTCTTTGTGGAGAGGACTTGATC
ATTAAAGGAATCAGCGTTCATATATCCAATGCCGAACCTAAGCACAATAG
CAATAGACAGTTAGAAAGAAGTGGAAGATTTGGTGGTAATCCAGGTGGCT
TTGGGAATCAGGGTGGATTTGGTAATAGCAGAGGGGGTGGAGCTGGTTTG
GGAAACAATCAAGGTAGTAATATGGGTGGTGGGATGAACTTTGGTGCGTT
CAGCATTAATCCAGCCATGATGGCTGCCGCCCAGGCAGCACTACAGAGCA
GTTGGGGTATGATGGGCATGTTAGCCAGCCAGCAGAACCAGTCAGGCCCA
TCGGGTAATAACCAAAACCAAGGCAACATGCAGAGGGAGCCAAACCAGGC
CTTCGGTTCTGGAAATAACTCTTATAGTGGCTCTAATTCTGGTGCAGCAA
TTGGTTGGGGATCAGCATCCAATGCAGGGTCGGGCAGTGGTTTTAATGGA
GGCTTTGGCTCAAGCATGGATTCTAAGTCTTCTGGCTGGGGAATGTAGGC
GGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSEYIRV
TEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLV
EGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLI
VLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYET
QVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDEL
REFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHI
SNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNM
GGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQG
NMQREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSGFNGGFGSSMDS
KSSGWGM*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5-FRT/TO-GFP-TARDBP4366.87 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU20768
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: No native initiating methionine; N terminally GFP tagged DTP43 for mammalian expression Flp-In vector; ; Estimated MW of expressed protein : 71934 Daltons
• Low Temp: No