cDNA Clone Ubiquitin

• Plasmid: pGEX-TEV-Ubiquitin (c220a mutant) duplicated TEV site
• Parent Plasmid: pGEX TEV TEV
• Expressed: GST-Ubiquitin (c220a mutant)
• Genbank: P62988*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGGTATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCAC
TCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCC
AAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGG
AAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGA
GTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQDHPPKSDENLYFQGGSGMQIFVKT
LTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLS
DYNIQKESTLHLVLRLRGG**

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20756
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Differs from reference sequence in having silent mutation at Arg73 ; GST - ubiquitin (Arg73 codon AGA mutated to more common CGA) with 2 TEV cleavage sites; ; Estimated MW of expressed protein : 36873 Daltons; Parental: 20712