cDNA Clone WDR28

• Plasmid: pET156P-1-WDR28
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-WDR28
• Genbank: NP_065890.1
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCGGCCCATCACCGGCAGAACACAGCAGGGCGGAGGAA
AGTGCAGGTTTCCTATGTTATTCGAGATGAAGTGGAGAAGTACAACCGAA
ATGGAGTCAATGCTCTGCAGCTGGATCCAGCACTAAATAGACTTTTCACA
GCCGGTCGAGACTCTATCATAAGAATATGGAGTGTCAATCAGCACAAGCA
AGATCCATATATAGCATCTATGGAACACCATACTGATTGGGTAAACGACA
TTGTACTCTGTTGTAATGGGAAAACATTAATATCTGCTTCTTCTGACACG
ACAGTAAAAGTATGGAATGCACACAAGGGATTTTGCATGTCAACATTAAG
GACACATAAGGATTACGTAAAGGCCTTAGCATATGCCAAGGATAAAGAAC
TAGTAGCATCAGCTGGGTTGGACAGACAAATATTCCTTTGGGATGTGAAT
ACTCTAACAGCATTGACTGCCTCAAATAACACTGTCACAACTTCTTCTTT
AAGTGGAAACAAAGATTCCATTTATAGCCTGGCCATGAATCAACTGGGAA
CAATCATTGTATCAGGGTCCACTGAAAAGGTGTTACGGGTATGGGATCCA
AGAACATGTGCAAAACTAATGAAGCTTAAAGGGCACACGGATAATGTGAA
GGCATTGCTATTAAACAGAGATGGCACGCAATGCCTGTCAGGCAGTTCTG
ATGGGACAATTCGCCTTTGGTCCCTTGGCCAGCAGAGATGTATAGCAACA
TACCGAGTCCATGATGAAGGTGTTTGGGCGCTGCAAGTCAATGATGCCTT
CACACATGTGTATTCTGGTGGAAGGGACAGGAAGATTTATTGTACAGACC
TAAGAAACCCTGACATTCGGGTGCTAATTTGTGAAGAAAAAGCACCAGTT
CTCAAGATGGAGCTTGATAGATCAGCTGATCCTCCTCCTGCAATTTGGGT
TGCAACAACTAAGTCTACAGTAAATAAATGGACTTTGAAAGGAATTCATA
ATTTTAGAGCCTCTGGAGATTATGACAATGACTGTACAAATCCTATAACA
CCTCTTTGTACACAACCTGACCAGGTTATTAAAGGGGGTGCTAGTATTAT
TCAGTGCCACATTCTTAATGATAAGAGACATATATTAACCAAAGATACCA
ATAATAATGTGGCATATTGGGATGTATTGAAGGCATGTAAAGTTGAAGAT
CTGGGCAAAGTGGATTTTGAAGATGAAATTAAGAAAAGATTTAAAATGGT
GTATGTGCCAAATTGGTTCTCAGTAGACTTAAAAACAGGGATGTTAACTA
TTACTTTGGATGAAAGTGATTGTTTTGCTGCCTGGGTTTCTGCAAAAGAT
GCTGGTTTCAGCAGCCCTGATGGGTCAGATCCAAAATTGAATTTAGGAGG
ACTTTTACTCCAAGCACTCCTGGAATATTGGCCTAGAACACATGTGAATC
CAATGGATGAAGAGGAAAATGAAGTAAACCATGTAAATGGGGAGCAGGAG
AACCGAGTGCAGAAGGGAAATGGATATTTTCAAGTGCCCCCACATACACC
CGTGATCTTTGGTGAAGCTGGAGGTCGCACACTGTTCAGGCTGCTCTGCC
GAGATTCCGGGGGTGAGACTGAGTCTATGCTTCTTAATGAAACAGTGCCA
CAATGGGTAATTGACATCACTGTGGATAAAAATATGCCCAAATTCAACAA
AATTCCTTTCTACCTCCAACCTCATGCATCTTCAGGAGCAAAAACCTTAA
AAAAAGATAGACTCTCTGCTAGTGACATGCTCCAAGTCCGAAAAGTTATG
GAACATGTTTATGAAAAAATTATCAACTTGGATAATGAGTCTCAAACCAC
TAGCTCTTCTAATAATGAAAAACCAGGAGAACAGGAAAAAGAAGAAGATA
TTGCTGTGTTGGCAGAGGAGAAAATTGAACTTTTGTGCCAAGACCAGGTT
TTGGATCCAAATATGGACCTTCGAACAGTGAAACACTTCATATGGAAGAG
CGGTGGAGACCTCACCCTCCATTACCGTCAGAAGTCCACGTGAGCGGCCG
C

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSPEFPGVDSKAAAMAAHHRQNTAGRRKV
QVSYVIRDEVEKYNRNGVNALQLDPALNRLFTAGRDSIIRIWSVNQHKQD
PYIASMEHHTDWVNDIVLCCNGKTLISASSDTTVKVWNAHKGFCMSTLRT
HKDYVKALAYAKDKELVASAGLDRQIFLWDVNTLTALTASNNTVTTSSLS
GNKDSIYSLAMNQLGTIIVSGSTEKVLRVWDPRTCAKLMKLKGHTDNVKA
LLLNRDGTQCLSGSSDGTIRLWSLGQQRCIATYRVHDEGVWALQVNDAFT
HVYSGGRDRKIYCTDLRNPDIRVLICEEKAPVLKMELDRSADPPPAIWVA
TTKSTVNKWTLKGIHNFRASGDYDNDCTNPITPLCTQPDQVIKGGASIIQ
CHILNDKRHILTKDTNNNVAYWDVLKACKVEDLGKVDFEDEIKKRFKMVY
VPNWFSVDLKTGMLTITLDESDCFAAWVSAKDAGFSSPDGSDPKLNLGGL
LLQALLEYWPRTHVNPMDEEENEVNHVNGEQENRVQKGNGYFQVPPHTPV
IFGEAGGRTLFRLLCRDSGGETESMLLNETVPQWVIDITVDKNMPKFNKI
PFYLQPHASSGAKTLKKDRLSASDMLQVRKVMEHVYEKIINLDNESQTTS
SSNNEKPGEQEKEEDIAVLAEEKIELLCQDQVLDPNMDLRTVKHFIWKSG
GDLTLHYRQKST*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20672
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express WDR28 with N-terminal 6HIS tag (contains preScission protease site); ; Estimated MW of expressed protein : 79826 Daltons