cDNA Clone Proteasome 26S non-ATPase subunit 4

• Synonyms: RPN10
• Plasmid: pET156P-1-Proteasome 26S non-ATPase subunit 4
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-Proteasome 26S non-ATPase subunit 4
• Genbank: NP_002801
• Tag: 6His
• Species: Human

Sequence of Insert

GTCGACATGGTGTTGGAAAGCACTATGGTGTGTGTGGACAACAGTGAGTA
TATGCGGAATGGAGACTTCTTACCCACCAGGCTGCAGGCCCAGCAGGATG
CTGTCAACATAGTTTGTCATTCAAAGACCCGCAGCAACCCTGAGAACAAC
GTGGGCCTTATCACACTGGCTAATGACTGTGAAGTGCTGACCACACTCAC
CCCAGACACTGGCCGTATCCTGTCCAAGCTACATACTGTCCAACCCAAGG
GCAAGATCACCTTCTGCACGGGCATCCGCGTGGCCCATCTGGCTCTGAAG
CACCGACAAGGCAAGAATCACAAGATGCGCATCATTGCCTTTGTGGGAAG
CCCAGTGGAGGACAATGAGAAGGATCTGGTGAAACTGGCTAAACGCCTCA
AGAAGGAGAAAGTAAATGTTGACATTATCAATTTTGGGGAAGAGGAGGTG
AACACAGAAAAGCTGACAGCCTTTGTAAACACGTTGAATGGCAAAGATGG
AACCGGTTCTCATCTGGTGACAGTGCCTCCTGGGCCCAGTTTGGCTGATG
CTCTCATCAGTTCTCCGATTTTGGCTGGTGAAGGTGGTGCCATGCTGGGT
CTTGGTGCCAGTGACTTTGAATTTGGAGTAGATCCCAGTGCTGATCCTGA
GCTGGCCTTGGCCCTTCGTGTATCTATGGAAGAGCAGCGGCAGCGGCAGG
AGGAGGAGGCCCGGCGGGCAGCTGCAGCTTCTGCTGCTGAGGCCGGGATT
GCTACGACTGGGACTGAAGACTCAGACGATGCCCTGCTGAAGATGACCAT
CAGCCAGCAAGAGTTTGGCCGCACTGGGCTTCCTGACCTAAGCAGTATGA
CTGAGGAAGAGCAGATTGCTTATGCCATGCAGATGTCCCTGCAGGGAGCA
GAGTTTGGCCAGGCGGAATCAGCAGACATTGATGCCAGCTCAGCTATGGA
CACATCTGAGCCAGCCAAGGAGGAGGATGATTACGACGTGATGCAGGACC
CCGAGTTCCTTCAGAGTGTCCTAGAGAACCTCCCAGGTGTGGATCCCAAC
AATGAAGCCATTCGAAATGCTATGGGCTCCCTGGCCTCCCAGGCCACCAA
GGACGGCAAGAAGGACAAGAAGGAGGAAGACAAGAAGTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSPEFPGVDMVLESTMVCVDNSEYMRNGD
FLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGR
ILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDN
EKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHL
VTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDPSADPELALAL
RVSMEEQRQRQEEEARRAAAASAAEAGIATTGTEDSDDALLKMTISQQEF
GRTGLPDLSSMTEEEQIAYAMQMSLQGAEFGQAESADIDASSAMDTSEPA
KEEDDYDVMQDPEFLQSVLENLPGVDPNNEAIRNAMGSLASQATKDGKKD
KKEEDKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20525
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express Proteasome 26S non-ATPase subunit 4 with N-terminal 6HIS tag (contains preScission protease site); ; Estimated MW of expressed protein : 43923 Daltons