cDNA Clone UBE2W

• Plasmid: pET28a-UBE2W isoform 1
• Parent Plasmid: pET28a original
• Expressed: 6His-UBE2W
• Genbank: NM_001001481.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGTCAATGCAGACCACAGGAAGGAGGGTGGAAGTATGGTT
TCCAAAACGACTACAGAAAGAACTGTTGGCTTTGCAAAATGACCCACCTC
CTGGAATGACCTTAAATGAGAAGAGTGTTCAAAATTCAATTACACAGTGG
ATTGTAGACATGGAAGGTGCACCAGGTACCTTATATGAAGGGGAAAAATT
TCAACTTCTATTTAAATTTAGTAGTCGATATCCTTTTGACTCTCCTCAGG
TCATGTTTACTGGTGAAAATATTCCTGTTCATCCTCATGTTTATAGCAAT
GGTCATATCTGTTTATCCATTCTAACAGAAGACTGGTCCCCAGCGCTCTC
AGTCCAATCAGTTTGTCTTAGCATTATTAGCATGCTTTCCAGCTGCAAGG
AAAAGAGACGACCACCGGATAATTCTTTTTATGTGCGAACATGTAACAAG
AATCCAAAGAAAACAAAATGGTGGTATCATGATGATACTTGTTGAGCGGC
CGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMASMQTTGRRVEVWFP
KRLQKELLALQNDPPPGMTLNEKSVQNSITQWIVDMEGAPGTLYEGEKFQ
LLFKFSSRYPFDSPQVMFTGENIPVHPHVYSNGHICLSILTEDWSPALSV
QSVCLSIISMLSSCKEKRRPPDNSFYVRTCNKNPKKTKWWYHDDTC*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU20190
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express UBE2W with N-terminal 6HIS tag (contains Thrombin cleavage site); ; Estimated MW of expressed protein : 22205 Daltons