cDNA Clone HECW2

• Plasmid: pEBG6P-HECW2
• Parent Plasmid: pEBG6P
• Expressed: GST-HECW2
• Genbank: NM_020760.1*
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCTAGTTCAGCCCGGGAGCACCTGCTTTTTGTGAGGCGTCGAAATCC
CCAGATGCGGTACACATTGAGCCCAGAGAACCTCCAGAGCCTTGCCGCCC
AGAGCTCCATGCCAGAGAACATGACCCTGCAGCGGGCCAACAGCGACACC
GACCTGGTGACTTCTGAGAGCCGCTCCAGCTTAACTGCCAGCATGTACGA
GTACACGCTGGGGCAAGCCCAGAACCTCATTATCTTCTGGGACATTAAAG
AGGAGGTGGACCCCAGTGATTGGATTGGACTTTATCATATAGATGAGAAT
TCTCCAGCCAACTTCTGGGATTCTAAGAACAGGGGTGTGACTGGAACACA
AAAAGGGCAAATTGTATGGAGAATTGAGCCTGGGCCCTATTTCATGGAAC
CGGAGATAAAAATCTGTTTTAAATATTACCACGGCATTAGTGGAGCCCTG
CGAGCCACGACCCCCTGCATCACCGTGAAGAACCCAGCTGTGATGATGGG
GGCAGAAGGCATGGAGGGAGGTGCTTCAGGAAACCTGCATTCTCGAAAAC
TTGTTAGCTTTACATTGTCAGATCTTAGGGCAGTTGGGCTAAAGAAAGGG
ATGTTCTTCAATCCTGACCCTTATCTTAAGATGTCAATTCAGCCAGGAAA
GAAGAGCAGTTTCCCCACCTGTGCCCACCACGGGCAGGAGAGACGGTCTA
CTATCATCAGTAACACCACCAATCCAATTTGGCACCGAGAGAAATATTCC
TTTTTTGCACTTCTTACTGATGTCTTAGAAATTGAAATTAAAGACAAATT
TGCCAAGAGCCGTCCCATCATCAAGCGTTTTCTGGGGAAACTAACCATTC
CAGTCCAGAGGCTGCTGGAGCGACAAGCCATCGGTGATCAAATGCTCAGC
TACAACCTTGGCAGAAGGCTCCCAGCTGACCACGTGAGTGGGTACCTCCA
GTTTAAAGTGGAGGTTACGTCTTCTGTTCATGAAGATGCCTCTCCAGAAG
CTGTTGGCACAATACTTGGAGTCAATTCTGTGAATGGAGACTTAGGTAGC
CCTTCCGATGACGAGGACATGCCAGGGAGCCATCACGACAGCCAGGTGTG
CTCTAATGGGCCAGTTTCTGAGGACAGTGCTGCCGATGGAACCCCCAAGC
ATTCATTCAGGACTAGCTCCACTCTGGAAATAGACACAGAGGAATTAACC
TCTACGTCTTCAAGGACCTCACCTCCCAGAGGACGTCAGGATTCACTCAA
TGATTACTTAGATGCTATCGAACACAATGGCCACTCCAGGCCAGGGACAG
CGACCTGCTCTGAGAGGTCCATGGGTGCCTCTCCGAAACTGAGGAGTAGT
TTTCCCACTGACACAAGACTCAATGCCATGCTTCATATTGATTCAGATGA
AGAAGATCACGAGTTCCAGCAAGACCTGGGTTACCCATCTTCCTTGGAGG
AGGAGGGAGGCCTGATCATGTTTAGCAGGGCATCCAGAGCTGATGATGGA
AGCCTGACATCTCAGACAAAGCTGGAGGACAACCCTGTTGAGAATGAGGA
AGCCTCCACACACGAAGCTGCTTCCTTTGAGGATAAGCCAGAAAATCTTC
CAGAGCTTGCAGAGAGCTCCTTACCTGCAGGCCCAGCCCCAGAGGAAGGT
GAAGGCGGCCCAGAGCCTCAACCCAGTGCTGACCAGGGCAGTGCTGAACT
GTGTGGCTCTCAAGAGGTAGATCAGCCCACAAGTGGCGCAGACACAGGGA
CTTCCGATGCATCAGGAGGAAGCCGAAGAGCCGTCAGTGAGACCGAGTCT
CTCGATCAGGGCTCTGAGCCTTCCCAGGTGTCCTCTGAAACAGAACCCAG
TGATCCTGCCAGGACAGAGAGTGTGAGCGAAGCCAGCACCAGGCCTGAGG
GAGAGAGTGACCTGGAATGCGCTGACAGCTCCTGCAATGAGAGTGTGACC
ACGCAGCTGTCCTCTGTGGACACACGGTGCTCCTCTCTTGAGAGCGCACG
GTTTCCTGAAACCCCAGCCTTTTCTTCTCAGGAGGAGGAAGACGGAGCCT
GTGCAGCAGAGCCCACCAGCAGTGGCCCTGCCGAAGGGTCGCAGGAATCC
GTGTGCACTGCTGGTTCTTTACCTGTGGTACAGGTGCCCAGTGGGGAGGA
TGAAGGGCCAGGGGCAGAATCGGCCACTGTACCTGACCAGGAGGAGCTGG
GGGAGGTCTGGCAGCGGAGGGGGAGCCTGGAGGGAGCTGCAGCTGCTGCC
GAGAGCCCACCGCAAGAAGAAGGCAGTGCTGGGGAGGCCCAAGGCACCTG
TGAAGGGGCAACTGCCCAGGAGGAGGGCGCTACTGGAGGTTCTCAAGCCA
ACGGCCACCAGCCACTGCGATCACTACCTTCAGTGCGCCAGGATGTTAGC
CGGTACCAGAGGGTGGACGAGGCTCTCCCACCAAACTGGGAGGCACGCAT
TGACAGCCACGGCAGGATCTTCTACGTGGATCACGTAAACAGAACCACGA
CGTGGCAGCGACCGACAGCTCCCCCAGCCCCGCAGGTGCTGCAGAGATCT
AACTCCATACAGCAAATGGAGCAGCTGAACCGGCGATATCAGAGTATCCG
CAGAACCATGACCAATGAGAGGCCTGAGGAAAATACCAATGCTATTGATG
GGGCAGGGGAGGAAGCTGACTTTCACCAAGCCAGTGCAGATTTCCGACGG
GAGAACATCCTGCCTCACTCTACCTCCAGATCCAGGATCACGTTGCTGTT
ACAGTCTCCCCCCGTGAAGTTCCTCATCAGCCCAGAGTTCTTCACCGTGC
TGCATTCTAACCCTAGTGCCTACCGCATGTTTACAAACAACACGTGTTTG
AAGCACATGATCACCAAAGTCCGGAGGGACACCCACCACTTTGAACGCTA
CCAGCATAACCGCGACCTTGTGGGATTCCTCAACATGTTCGCGAACAAAC
AGCTAGAGCTGCCGCGGGGATGGGAAATGAAACATGATCACCAGGGCAAG
GCATTCTTTGTGGACCACAACTCCCGCACCACCACTTTCATTGATCCCCG
GCTCCCACTTCAGAGCAGTAGACCCACAAGTGCGCTGGTTCATCGGCAAC
ACCTGACAAGGCAACGCAGCCACAGTGCGGGTGAGGTAGGAGAAGATTCT
CGACATGCAGGACCACCAGTTCTTCCCAGGCCATCCAGTACATTCAATAC
AGTCAGTAGGCCACAGTACCAGGACATGGTTCCAGTGGCTTACAATGACA
AGATTGTTGCATTTCTACGTCAACCCAATATCTTTGAAATTCTACAGGAG
CGTCAACCTGATCTTACCAGGAATCACTCACTCAGGGAGAAGATCCAATT
TATCCGAACTGAAGGGACTCCAGGATTGGTGCGCCTTTCAAGCGATGCAG
ACCTTGTTATGTTGCTGAGCTTATTTGAAGAAGAGATAATGTCATATGTG
CCTCCACATGCCTTACTCCACCCCAGCTACTGTCAGTCCCCACGTGGCTC
TCCCGTGTCATCTCCTCAGAACTCGCCAGGTACCCAGCGTGCCAATGCCC
GGGCTCCAGCCCCTTACAAGCGGGATTTCGAAGCCAAACTGAGGAACTTT
TACAGGAAGTTAGAGACTAAAGGATATGGACAAGGCCCAGGGAAGTTAAA
GTTAATTATCCGAAGAGATCACTTACTAGAAGATGCTTTTAATCAGATTA
TGGGCTACTCCAGAAAAGACCTGCAGAGAAATAAGCTATATGTCACCTTC
GTTGGGGAGGAAGGGCTGGATTACAGTGGGCCTTCTAGAGAGTTTTTCTT
CCTGGTATCCAGAGAACTCTTTAACCCATATTATGGCTTATTTGAATATT
CAGCCAATGACACATACACAGTACAAATAAGTCCTATGTCTGCTTTTGTA
GACAATCACCATGAATGGTTCCGATTCAGTGGTAGGATCCTTGGTCTTGC
ACTAATACACCAGTATTTGTTGGATGCCTTCTTCACACGGCCCTTTTATA
AGGCTCTTCTCAGAATTCTATGTGACCTGAGTGACCTAGAATACCTTGAT
GAAGAGTTCCATCAGAGCCTGCAGTGGATGAAAGACAATGATATCCATGA
CATCCTAGACCTCACGTTCACTGTGAACGAAGAAGTATTTGGGCAGATAA
CTGAACGAGAATTAAAGCCAGGGGGTGCCAATATCCCAGTTACAGAGAAG
AACAAGAAGGAGTACATCGAGAGGATGGTGAAGTGGAGGATTGAGAGGGG
TGTTGTACAGCAAACAGAGAGCTTAGTGCGTGGCTTCTATGAGGTGGTGG
ATGCCAGGCTGGTATCTGTTTTTGATGCAAGAGAACTGGAATTGGTCATC
GCAGGCACAGCTGAAATAGACCTAAGTGATTGGAGAAACAACACAGAATA
TAGAGGAGGATACCATGACAATCATATTGTAATTCGGTGGTTCTGGGCTG
CAGTGGAAAGATTCAACAATGAACAACGACTAAGGTTGTTACAGTTTGTT
ACAGGCACATCCAGCATTCCCTATGAAGGATTTGCTTCACTCCGAGGGAG
TAACGGCCCAAGAAGATTCTGTGTGGAGAAATGGGGGAAAATCACTGCTC
TTCCCAGAGCGCATACATGTTTTAACCGTCTGGATCTGCCTCCCTACCCA
TCCTTTTCCATGCTTTATGAAAAACTGTTGACAGCAGTTGAAGAAACCAG
TACTTTTGGACTTGAGTGA

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDKLDIHCG
IRPWAAAVDMASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENM
TLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDW
IGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFK
YYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSD
LRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTN
PIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLER
QAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEDASPEAVGTILGV
NSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSFRTSST
LEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSM
GASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMF
SRASRADDGSLTSQTKLEDNPVENEEASTHEAASFEDKPENLPELAESSL
PAGPAPEEGEGGPEPQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGS
RRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECA
DSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSS
GPAEGSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQEELGEVWQRRG
SLEGAAAAAESPPQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRS
LPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAP
PAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADF
HQASADFRRENILPHSTSRSRITLLLQSPPVKFLISPEFFTVLHSNPSAY
RMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGW
EMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSH
SAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQ
PNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSL
FEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKR
DFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDL
QRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTV
QISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILC
DLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPG
GANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVF
DARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNE
QRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCF
NRLDLPPYPSFSMLYEKLLTAVEETSTFGLE*

• Vector Type: Mammalian
• DU Number: DU20185
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express HECW2 (wild type) with N-terminal GST (contains precission protease site); Silent mutations at g384a (Gly128), c694a (Arg232); ; Estimated MW of expressed protein : 205526 Daltons