cDNA Clone Triad1

• Plasmid: pGEX6P-1 Triad1 200-493
• Parent Plasmid: pGEX6P1
• Expressed: GST-Triad1 200-493
• Genbank: NM_006321*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCTTGCTTCCCAATGAAGAATTGAGAGAGAAATACAGGCGCTACCT
CTTCAGGGACTATGTGGAGAGTCATTACCAGCTCCAGCTGTGCCCTGGTG
CAGACTGCCCCATGGTTATTCGGGTACAGGAGCCTAGAGCTCGCCGAGTA
CAGTGCAATCGGTGCAACGAGGTCTTCTGTTTCAAGTGTCGTCAGATGTA
TCACGCACCCACAGACTGTGCCACAATCCGGAAATGGCTCACGAAGTGTG
CAGACGACTCTGAAACAGCCAACTACATTAGTGCTCACACTAAAGACTGT
CCCAAGTGCAACATCTGCATTGAGAAGAATGGAGGCTGCAATCACATGCA
ATGCTCCAAATGTAAACACGACTTCTGCTGGATGTGTCTAGGAGATTGGA
AGACTCATGGCAGTGAATACTATGAGTGCAGTCGTTACAAGGAGAATCCT
GACATCGTGAACCAGAGCCAACAAGCCCAGGCGAGGGAAGCCCTCAAGAA
GTACTTATTCTACTTTGAGAGGTGGGAAAACCACAATAAAAGCTTGCAGC
TAGAGGCACAGACATACCAGCGGATTCACGAGAAGATTCAGGAGAGGGTC
ATGAACAATCTGGGGACATGGATCGACTGGCAGTACCTACAGAATGCTGC
CAAGCTCTTGGCCAAGTGTCGATACACCCTGCAATACACCTACCCATATG
CATATTACATGGAGTCCGGACCCAGGAAGAAGCTGTTTGAATACCAGCAG
GCTCAGCTGGAGGCTGAGATCGAAAACCTCTCATGGAAAGTGGAGCGTGC
AGACAGCTATGACAGAGGGGACTTGGAGAACCAGATGCATATAGCGGAGC
AGCGGAGGAGAACCCTGCTGAAAGATTTCCATGACACCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSLLPNEELREKYRRYLFRDYVESHYQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCATIRKWLTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDFCWMCLGDWKTHGSEYYECSRYKENPDIVNQSQQAQAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEKIQERVMNNLGTWIDWQYLQNAAKLLAKCRYTLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDLENQMHIAEQRRRTLLKDFHDT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20157
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for GST- Triad1 aa200-493.; Clone contains nt 598 – 1482 of the reference sequence plus artificial STOP codon.; ; Estimated MW of expressed protein : 62272 Daltons