cDNA Clone UBE2Z

• Synonyms: ubiquitin-conjugating enzyme E2Z (UBE2Z), Use1
• Plasmid: pET15b 6HIS C3 UBE2Z
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-UBE2Z
• Genbank: NM_023079.3 GI:150417998*
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGGAGAGTCCGACTGAGGAGGCGGCAACGGCGGGCGCCGGGGCGGCGGGCCCCGGGGCGAGCAGCGTTGCTGGTGTTGTTGGCGTTAGCGGCAGCGGCGGCGGGTTCGGGCCGCCTTTCCTGCCGGATGTGTGGGCGGCGGCGGCGGCAGCGGGCGGGGCCGGGGGCCCGGGGAGCGGCCTGGCTCCGCTGCCCGGGCTCCCGCCCTCAGCCGCTGCCCACGGGGCCGCGCTGCTTAGCCACTGGGACCCCACGCTCAGCTCCGACTGGGACGGCGAGCGCACCGCGCCGCAGTGT
CTACTCCGGATCAAGCGGGATATCATGTCCATTTATAAGGAGCCTCCTCC
AGGAATGTTCGTTGTACCTGATACTGTTGACATGACTAAGATTCATGCAT
TGATCACAGGCCCATTTGACACTCCTTATGAAGGGGGTTTCTTCCTGTTC
GTGTTTCGGTGTCCGCCCGACTATCCCATCCACCCACCTCGGGTCAAACT
GATGACAACGGGCAATAACACAGTGAGGTTTAACCCCAACTTCTACCGCA
ATGGGAAAGTCTGCTTGAGTATTCTAGGTACATGGACTGGACCTGCCTGG
AGCCCAGCCCAGAGCATCTCCTCAGTGCTCATCTCTATCCAGTCCCTGAT
GACTGAGAACCCCTATCACAATGAGCCCGGCTTTGAACAGGAGAGACATC
CAGGAGACAGCAAAAACTATAATGAATGTATCCGGCACGAGACCATCAGA
GTTGCAGTCTGTGACATGATGGAAGGAAAGTGTCCCTGTCCTGAACCCCT
ACGAGGGGTGATGGAGAAGTCCTTTCTGGAGTATTACGACTTCTATGAGG
TGGCCTGCAAAGATCGCCTGCACCTTCAAGGCCAAACTATGCAGGACCCT
TTTGGAGAGAAGCGGGGCCACTTTGACTACCAGTCCCTCTTGATGCGCCT
GGGACTGATACGTCAGAAAGTGCTGGAGAGGCTCCATAATGAGAATGCAG
AAATGGACTCTGATAGCAGTTCATCTGGGACAGAGACAGACCTTCATGGG
AGCCTGAGGGTTTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMAESPTEEAATAGAGAAGPGASSVAGV
VGVSGSGGGFGPPFLPDVWAAAAAAGGAGGPGSGLAPLPGLPPSAAAHGA
ALLSHWDPTLSSDWDGERTAPQCLLRIKRDIMSIYKEPPPGMFVVPDTVD
MTKIHALITGPFDTPYEGGFFLFVFRCPPDYPIHPPRVKLMTTGNNTVRF
NPNFYRNGKVCLSILGTWTGPAWSPAQSISSVLISIQSLMTENPYHNEPG
FEQERHPGDSKNYNECIRHETIRVAVCDMMEGKCPCPEPLRGVMEKSFLE
YYDFYEVACKDRLHLQGQTMQDPFGEKRGHFDYQSLLMRLGLIRQKVLER
LHNENAEMDSDSSSSGTETDLHGSLRV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20121
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NM_023079.3 CDS: c846t (silent); ; Estimated MW of expressed protein : 40655 Daltons