cDNA Clone
UBE4a

Plasmid
pFBHT-UBE4a
Parent Plasmid
pFastBac HTb
Expressed
6His-UBE4a
Genbank
NM_004788
Tag
6His
Species
Human
Sequence of Insert
GCGGCCGCGATGACAGACCAGGAGAATAACAACAACATCTCAAGTAACCCCTTTGCTGCTCTTTTTGGCTCCCTGGCTGATGCCAAACAGTTTGCGGCAATCCAAAAAGAGCAGCTGAAGCAACAATCTGATGAACTCCCAGCTAGCCCAGATGACTCGGATAATAGCGTGTCAGAGAGCCTGGATGAATTCGATTACTCTGTGGCTGAGATTAGCCGCTCATTCCGATCACAGCAGGAAATATGTGAGCAACTCAACATCAATCACATGATCCAAAGGATCTTCCTTATTACTCTGGACAACAGTGATCCCAGCTTGAAAAGCGGGAATGGCATCCCTAGCCGTTGTGTGTATTTGGAAGAAATGGCAGTAGAGCTAGAAGATCAAGACTGGCTTGATATGAGCAATGTTGAGCAGGCCCTCTTCGCTCGCTTATTACTTCAAGATCCAGGCAACCAC
TTAATTAACATGACTTCTTCTACAACGCTAAATCTCTCTGCTGATCGAGA
TGCAGGAGAGAGGCACATTTTTTGTTACCTTTACTCCTGCTTCCAGAGAG
CCAAGGAAGAGATTACCAAAGTTCCAGAGAACCTGCTACCCTTTGCAGTG
CAGTGCAGAAACCTCACTGTGTCCAATACCCGAACAGTTCTTCTCACCCC
AGAGATCTATGTTGACCAAAACATCCATGAGCAACTGGTAGATTTGATGT
TAGAAGCCATCCAGGGAGCCCGTGAGTACATGAACAAGATCTATTTTGAA
GATGTAACTGAGTTTCTGGAAGAGGTCATTGAAGCCTTGATATTGGATGA
GGAAGTTAGAACATTTCCAGAAGTCATGATTCCAGTGTTTGATATTTTAT
TGGGCCGAATAAAAGATCTAGAGCTCTGTCAGATCCTTTTGTATGCATAT
CTGGATATTCTTCTCTATTTCACTAGGCAAAAAGATATGGCAAAGGTTTT
TGTAGAATACATTCAGCCCAAGGACCCTACCAATGGGCAAATGTACCAGA
AGACCTTGCTGGGAGTAATTCTGAGTATCTCCTGCTTATTAAAGACTCCG
GGTGTTGTAGAAAATCATGGCTACTTTTTGAATCCATCTCGTTCCAGCCC
CCAGGAGATCAAAGTACAGGAGGCCAACATCCATCAGTTCATGGCTCAGT
TCCACGAAAAGATCTACCAGATGCTGAAGAACTTACTCCAGCTCTCTCCA
GAAACCAAACACTGTATCTTGTCCTGGCTTGGAAACTGTTTGCATGCAAA
TGCAGGCCGCACCAAGATTTGGGCCAATCAGATGCCAGAAATCTTTTTCC
AAATGTATGCCTCAGATGCTTTCTTTCTGAATCTGGGTGCTGCTCTCCTG
AAGCTATGCCAGCCATTTTGCAAACCCAGATCCTCTCGGCTCCTCACCTT
TAATCCCACATACTGTGCCCTCAAGGAGTTGAATGATGAAGAACGAAAAA
TTAAAAATGTACACATGAGAGGTTTGGACAAAGAAACCTGTTTGATCCCA
GCTGTGCAGGAGCCGAAGTTTCCACAGAACTACAACCTTGTAACAGAGAA
CCTTGCTCTGACAGAGTACACCTTGTACTTGGGATTTCACAGGTTGCATG
ATCAGATGGTAAAAATCAACCAAAATCTGCATCGGCTGCAGGTTGCCTGG
CGGGATGCTCAGCAAAGTTCTAGCCCTGCTGCTGACAATCTTCGTGAGCA
GTTTGAACGACTGATGACCATCTATCTTTCTACCAAGACTGCCATGACAG
AGCCACAAATGCTACAAAACTGCCTAAACTTGCAGGTGTCCATGGCTGTT
CTACTGGTTCAACTGGCCATAGGCAATGAGGGCTCACAGCCAATAGAGCT
AACCTTTCCTTTGCCAGATGGCTACAGCTCTTTGGCTTATGTGCCAGAAT
TTTTTGCAGATAACCTGGGTGATTTTCTCATTTTTCTCCGCCGCTTTGCC
GATGACATTTTGGAGACATCAGCAGATTCCCTGGAGCATGTCCTTCACTT
TATCACCATTTTCACTGGAAGCATAGAAAGAATGAAGAATCCCCACCTGA
GGGCCAAACTAGCAGAGGTGTTGGAAGCAGTGATGCCCCACCTGGATCAG
ACCCCAAATCCCTTGGTATCCAGTGTGTTCCACCGGAAACGTGTGTTCTG
CAACTTTCAGTATGCACCCCAACTTGCAGAGGCTCTAATCAAGGTTTTTG
TGGACATCGAATTTACAGGAGACCCCCATCAATTTGAACAGAAGTTTAAT
TACCGCCGTCCCATGTATCCTATCCTAAGATACATGTGGGGGACAGATAC
CTATCGGGAGAGCATTAAGGATTTGGCTGACTATGCCTCTAAGAATTTAG
AAGCCATGAATCCCCCACTTTTCCTCCGCTTTCTTAACCTGCTAATGAAT
GATGCCATCTTCCTTTTGGATGAAGCCATACAGTATTTGAGCAAGATAAA
GATTCAGCAAATTGAGAAGGATCGAGGTGAATGGGATAGTCTGACTCCAG
AAGCCCGCCGAGAAAAGGAGGCTGGCCTACAGATGTTTGGACAGCTGGCA
CGTTTCCATAACATCATGTCCAATGAAACAATCGGTACCCTTGCCTTTCT
CACATCAGAGATCAAGTCACTCTTTGTGCATCCCTTCCTGGCTGAGCGCA
TCATCTCCATGTTGAACTACTTCCTGCAACACCTGGTTGGCCCCAAGATG
GGTGCCTTAAAAGTCAAGGACTTCAGCGAATTTGACTTCAAACCCCAGCA
GCTTGTATCAGATATCTGCACTATCTACTTAAATCTTGGGGATGAGGAGA
ATTTCTGTGCCACTGTGCCCAAGGATGGACGTTCCTATTCCCCAACTCTC
TTTGCACAGACAGTTCGAGTCTTGAAGAAAATAAATAAGCCTGGGAATAT
GATTATGGCTTTCAGCAACTTGGCAGAGAGAATCAAGTCTCTTGCAGACC
TCCAACAACAGGAAGAGGAAACCTATGCAGATGCCTGTGATGAGTTCCTG
GATCCCATTATGAGCACACTGATGTGTGACCCTGTGGTGCTGCCATCTTC
CAGAGTCACTGTGGATAGATCCACCATTGCAAGACATTTGCTCAGTGACC
AAACAGATCCCTTTAACCGTAGTCCCCTCACCATGGACCAGATCCGGCCA
AACACAGAACTAAAAGAAAAAATCCAACGGTGGCTTGCAGAGAGGAAACA
ACAAAAGGAGCAACTTGAATAGGCGGCCGC

Amino Acid Sequence
MSYYHHHHHHDYDIPTTENLYFQGAMGSGIQRPTSTSSLVAAAMTDQENN
NNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSES
LDEFDYSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGN
GIPSRCVYLEEMAVELEDQDWLDMSNVEQALFARLLLQDPGNHLINMTSS
TTLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTV
SNTRTVLLTPEIYVDQNIHEQLVDLMLEAIQGAREYMNKIYFEDVTEFLE
EVIEALILDEEVRTFPEVMIPVFDILLGRIKDLELCQILLYAYLDILLYF
TRQKDMAKVFVEYIQPKDPTNGQMYQKTLLGVILSISCLLKTPGVVENHG
YFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCIL
SWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFC
KPRSSRLLTFNPTYCALKELNDEERKIKNVHMRGLDKETCLIPAVQEPKF
PQNYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSS
SPAADNLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAI
GNEGSQPIELTFPLPDGYSSLAYVPEFFADNLGDFLIFLRRFADDILETS
ADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVS
SVFHRKRVFCNFQYAPQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYP
ILRYMWGTDTYRESIKDLADYASKNLEAMNPPLFLRFLNLLMNDAIFLLD
EAIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFGQLARFHNIMS
NETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKD
FSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRV
LKKINKPGNMIMAFSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTL
MCDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEK
IQRWLAERKQQKEQLE*
Vector Type
Baculovirus
Antibiotic
Amp
DU Number
DU20020
Location
PPU
MTA
Notes
Baculovirus construct to express Ube4a with N-terminal 6HIS tag (contains TEV protease site)

Estimated MW of expressed protein : 128335 Daltons
Price
£125.00
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