cDNA Clone USP49

• Plasmid: pET28a-HALO_TEV-USP49 C262A
• Parent Plasmid: pET28a HALO TEV
• Expressed: HALO-USP49 C262A
• Tag: HALO

Sequence of Insert

ATGGATAGATGCAAACATGTAGGGCGGTTACGGCTCGCCCAGGACCACTCCATCCTGAACCCTCAGAAGTGGTGCTGCTTAGAGTGTGCCACCACCGAGTCCGTGTGGGCCTGCCTCAAGTGCTCCCACGTGGCCTGCGGCCGCTATATTGAGGACCACGCCCTGAAACACTTTGAGGAGACGGGACACCCGCTAGCCATGGAAGTCCGGGATCTCTACGTGTTCTGTTACCTGTGCAAGGACTACGTGCTCAATGATAACCCAGAGGGGGACCTGAAGCTGCTAAGAAGCTCCCTCCTGGCGGTCCGGGGCCAGAAACAGGACACGCCGGTGAGACGTGGGCGGACGCTGCGGTCCATGGCTTCGGGTGAGGACGTGGTCCTGCCGCAGCGCGCTCCTCAGGGACAGCCGCAGATGCTCACGGCTCTGTGGTACCGGCGTCAGCGCCTGCTGGCCAGGACGCTGCGGCTGTGGTTCGAGAAGAGCTCCCGGGGCCAGGCGAAGCTGGAGCAGCGGCGGCAGGAGGAGGCCCTGGAGCGCAAGAAGGAGGAGGCGCGGAGGCGGCGGCGCGAGGTGAAACGGCGGCTGCTGGAGGAGCTGGCCAGCACCCCTCCGCGCAAGAGTGCACGGCTGCTCCTGCACACGCCCCGCGACGCGGGCCCGGCTGCCTCGCGCCCCGCCGCCCTCCCTACCTCACGCAGAGTGCCCGCCGCCACACTCAAGCTGCGTCGCCAGCCGGCCATGGCCCCAGGCGTCACGGGCCTGCGCAACCTGGGCAACACCgcCTACATGAACTCCATCCTCCAGGTGCTCAGCCACCTCCAGAAGTTCCGAGAATGTTTCCTCAACCTTGACCCTTCCAAAACGGAACATCTGTTTCCCAAAGCCACCAACGGGAAGACTCAGCTTTCTGGCAAGCCAACCAACAGCTCGGCCACGGAGCTGTCCTTGAGAAATGACAGGGCCGAGGCATGCGAGCGGGAGGGCTTCTGCTGGAACGGCAGGGCCTCCATTAGTCGGAGTCTGGAGCTCATCCAGAACAAGGAGCCGAGTTCAAAGCACATTTCCCTCTGCCGTGAACTGCACACCCTCTTCCGAGTCATGTGGTCCGGGAAGTGGGCCCTAGTGTCGCCCTTCGCCATGCTGCACTCAGTGTGGAGCCTGATCCCTGCCTTCCGCGGCTACGACCAACAGGACGCGCAGGAATTTCTCTGCGAGCTGCTGCACAAGGTGCAGCAGGAACTCGAGTCTGAGGGCACCACACGCCGGATCCTCATCCCCTTCTCCCAGAGGAAGCTCACCAAACAGGTCTTAAAGGTGGTGAATACCATATTTCATGGGCAGCTGCTCAGTCAGGTCACATGTATATCATGCAATTACAAATCCAATACCATTGAGCCCTTTTGGGACCTATCCCTGGAATTCCCTGAACGCTATCACTGCATAGAAAAGGGGTTTGTCCCTTTGAATCAAACAGAGTGCTTGCTCACTGAGATGCTGGCCAAATTCACAGAGACAGAGGCCCTGGAAGGGAGAATCTACGCTTGTGACCAGTGTAACAGCAAACGACGAAAATCCAATCCCAAACCCCTTGTTCTGAGTGAAGCTAGAAAGCAGTTAATGATCTACAGACTACCTCAGGTTCTCCGGCTGCACCTTAAAAGATTCAGGTGGTCTGGCCGTAATCATCGAGAGAAGATTGGGGTCCATGTCGTCTTTGACCAGGTATTAACCATGGAACCTTACTGCTGCAGGGACATGCTCTCCTCTCTTGACAAAGAGACCTTTGCCTATGATCTCTCCGCAGTGGTCATGCATCACGGGAAAGGGTTTGGCTCAGGACACTACACAGCCTATTGCTACAACACAGAGGGAGGTGCGTGCGCTTTACTCTGTGGGGTGGGGGACACGGAAAGGGGTTGAGATCT

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGENLYFQGGSMDRCKHVGRLRLAQDHSILNPQKWCCLECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVRDLYVFCYLCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMASGEDVVLPQRAPQGQPQMLTALWYRRQRLLARTLRLWFEKSSRGQAKLEQRRQEEALERKKEEARRRRREVKRRLLEELASTPPRKSARLLLHTPRDAGPAASRPAALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTCYMNSILQVLSHLQKFRECFLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACEREGFCWNGRASISRSLELIQNKEPSSKHISLCRELHTLFRVMWSGKWALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGTTRRILIPFSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPERYHCIEKGFVPLNQTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSNPKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVVFDQVLTMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGACALLCGVGDTERG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU23278
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: TGC to GCC (C262A) compared to ref shown