cDNA Clone CAND1

• Synonyms: cullin-associated and neddylation-dissociated 1
• Plasmid: pGST-1 -(blunt)TEV-Cand1
• Parent Plasmid: pGST
• Expressed: GST-Cand1
• Genbank: NM_018448.3.
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACGCGAAAACCTGTATTTTCAGATGGCGAGCGCCTCGTACCACATTTCCAATTTGCTGGAAAAAATGACATCCAGCGACAAGGACTTTAGGTTTATGGCTACAAATGATTTGATGACGGAACTGCAGAAAGATTCCATCAAGTTGGATGATGATAGTGAAAGGAAAGTAGTGAAAATGATTTTGAAGTTATTGGAAGATAAAAATGGAGAGGTACAGAATTTAGCTGTCAAATGTCTTGGTCCTTTAGTGAGTAAAGTGAAAGAATACCAAGTAGAGACAATTGTAGATACCCTCTGCACTAACATGCTTTCTGATAAAGAACAACTTCGAGACATTTCAAGTATTGGTCTTAAAACAGTAATTGGAGAACTTCCTCCAGCTTCCAGTGGCTCTGCATTAGCTGCTAATGTATGTAAAAAGATTACTGGACGTCTTACAAGTGCAATAGCAAAACAGGAAGATGTCTCTGTTCAGCTAGAAGCCTTGGATATTATGGCTGATATGTTGAGCAGGCAAGGAGGACTTCTTGTTAATTTCCATCCTTCAATTCTGACCTGTCTACTTCCCCAGTTGACCAGCCCTAGACTTGCAGTGAGGAAAAGAACCATTATCGCTCTTGGCCATCTGGTTATGAGCTGTGGAAATATAGTTTTTGTAGATCTTATTGAACATCTGTTGTCAGAGTTGTCCAAAAATGATTCTATGTCAACAACAAGAACCTACATACAATGTATTGCTGCTATTAGTAGGCAAGCTGGTCATAGAATAGGTGAATACCTTGAGAAGATAATTCCTTTGGTGGTAAAATTTTGCAATGTAGATGATGATGAATTAAGAGAGTACTGTATTCAAGCCTTTGAATCATTTGTAAGAAGATGTCCTAAGGAAGTATATCCTCATGTTTCTACCATTATAAATATTTGTCTTAAATATCTTACCTATGATCCAAATTATAATTACGATGATGAAGATGAAGATGAAAATGCAATGGATGCTGATGGTGGTGATGATGATGATCAAGGGAGTGATGATGAATACAGTGATGATGATGACATGAGTTGGAAAGTGAGACGTGCAGCTGCGAAGTGCTTGGATGCTGTAGTTAGCACAAGGCATGAAATGCTTCCAGAATTCTACAAGACCGTCTCTCCTGCACTAATATCCAGATTTAAAGAGCGTGAAGAGAATGTAAAGGCAGATGTTTTTCACGCATACCTTTCTCTTTTGAAGCAAACTCGTCCTGTACAAAGTTGGCTATGTGACCCTGATGCAATGGAGCAGGGAGAAACACCTTTAACAATGCTTCAGAGTCAGGTTCCCAACATTGTTAAAGCTCTTCACAAACAGATGAAAGAAAAAAGTGTGAAGACCCGACAGTGTTGTTTTAACATGTTAACTGAGCTGGTAAATGTATTACCTGGGGCCCTAACTCAACACATTCCTGTACTTGTACCAGGAATCATTTTCTCACTGAATGATAAATCAAGCTCATCGAATTTGAAGATCGATGCTTTGTCATGTCTATACGTAATCCTCTGTAACCATTCTCCTCAAGTCTTCCATCCTCACGTTCAGGCTTTGGTTCCTCCAGTGGTGGCTTGTGTTGGAGACCCATTTTACAAAATTACATCTGAAGCACTTCTTGTTACTCAACAGCTTGTCAAAGTAATTCGTCCTTTAGATCAGCCTTCCTCGTTTGATGCAACTCCTTATATCAAAGATCTATTTACCTGTACCATTAAGAGATTAAAAGCAGCTGACATTGATCAGGAAGTCAAGGAAAGGGCTATTTCCTGTATGGGACAAATTATTTGCAACCTTGGAGACAATTTGGGTTCTGACTTGCCTAATACACTTCAGATTTTCTTGGAGAGACTAAAGAATGAAATTACCAGGTTAACTACAGTAAAGGCATTGACACTGATTGCTGGGTCACCTTTGAAGATAGATTTGAGGCCTGTTCTGGGAGAAGGGGTTCCTATCCTTGCTTCATTTCTTAGAAAAAACCAGAGAGCTTTGAAACTGGGTACTCTTTCTGCCCTTGATATTCTAATAAAAAACTATAGTGACAGCTTGACAGCTGCCATGATTGATGCAGTTCTAGATGAGCTCCCACCTCTTATCAGCGAAAGTGATATGCATGTTTCACAAATGGCCATCAGTTTTCTTACCACTTTGGCAAAAGTATATCCCTCCTCCCTTTCAAAGATAAGTGGATCCATTCTCAATGAACTTATTGGACTTGTGAGATCACCCTTATTGCAGGGGGGAGCTCTTAGTGCCATGCTAGACTTTTTCCAAGCTCTGGTTGTCACTGGAACAAATAATTTAGGATACATGGATTTGTTGCGCATGCTGACTGGTCCAGTTTACTCTCAGAGCACAGCTCTTACTCATAAGCAGTCTTATTATTCCATTGCCAAATGTGTAGCTGCCCTTACTCGAGCATGCCCTAAAGAGGGACCAGCTGTAGTAGGTCAGTTTATTCAAGATGTCAAGAACTCAAGGTCTACAGATTCCATTCGTCTCTTAGCTCTACTTTCTCTTGGAGAAGTTGGGCATCATATTGACTTAAGTGGACAGTTGGAACTAAAATCTGTAATACTAGAAGCTTTCTCATCTCCTAGTGAAGAAGTCAAATCAGCTGCATCCTATGCATTAGGCAGCATTAGTGTGGGCAACCTTCCTGAATATCTACCGTTTGTCCTGCAAGAAATAACTAGTCAACCCAAAAGGCAGTATCTTTTACTTCATTCCTTGAAGGAAATTATTAGCTCTGCATCAGTGGTGGGCCTTAAACCATATGTTGAAAACATCTGGGCCTTATTACTAAAGCACTGTGAGTGTGCAGAGGAAGGAACCAGAAATGTTGTTGCTGAATGTCTAGGAAAACTCACTCTAATTGATCCAGAAACTCTCCTTCCACGGCTTAAGGGGTACTTGATATCAGGCTCATCATATGCCCGAAGCTCAGTGGTTACGGCTGTGAAATTTACAATTTCTGACCATCCACAACCTATTGATCCACTGTTAAAGAACTGCATAGGTGATTTCCTAAAAACTTTGGAAGACCCAGATTTGAATGTGAGAAGAGTAGCCTTGGTCACATTTAATTCAGCAGCACATAACAAGCCATCATTAATAAGGGATCTATTGGATACTGTTCTTCCACATCTTTACAATGAAACAAAAGTTAGAAAGGAGCTTATAAGAGAGGTAGAAATGGGTCCATTTAAACATACGGTTGATGATGGTCTGGATATTAGAAAGGCAGCATTTGAGTGTATGTACACACTTCTAGACAGTTGTCTTGATAGACTTGATATCTTTGAATTTCTAAATCATGTTGAAGATGGTTTGAAGGACCATTATGATATTAAGATGCTGACATTTTTAATGTTGGTGAGACTGTCTACCCTTTGTCCAAGTGCAGTACTGCAGAGGTTGGACCGACTTGTTGAGCCATTACGTGCAACATGTACAACTAAGGTAAAGGCAAACTCAGTAAAGCAGGAGTTTGAAAAACAAGATGAATTAAAGCGATCTGCCATGAGAGCAGTAGCAGCACTACTAACCATTCCAGAAGCAGAGAAGAGTCCACTGATGAGTGAATTCCAGTCACAGATCAGTTCTAACCCTGAGCTGGCGGCTATCTTTGAAAGTATCCAGAAAGATTCATCATCTACTAACTTGGAATCAATGGACACTAGTTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSPEFPGRRENLYFQMASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPASSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINICLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAVVSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQGETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALLSLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTLIDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU23732
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sal1 +2bp