cDNA Clone CAND1

• Synonyms: cullin-associated and neddylation-dissociated 1
• Plasmid: pFastbacDual Dac TEV- Cand1
• Parent Plasmid: pFastbacDual DAC TEV
• Expressed: DAC-CAND1
• Genbank: NM_018448.3.
• Tag: DAC
• Species: Human

Sequence of Insert

gcggccgccATGGCGAGCGCCTCGTACCACATTTCCAATTTGCTGGAAAAAATGACATCCAGCGACAAGGACTTTAGGTTTATGGCTACAAATGATTTGATGACGGAACTGCAGAAAGATTCCATCAAGTTGGATGATGATAGTGAAAGGAAAGTAGTGAAAATGATTTTGAAGTTATTGGAAGATAAAAATGGAGAGGTACAGAATTTAGCTGTCAAATGTCTTGGTCCTTTAGTGAGTAAAGTGAAAGAATACCAAGTAGAGACAATTGTAGATACCCTCTGCACTAACATGCTTTCTGATAAAGAACAACTTCGAGACATTTCAAGTATTGGTCTTAAAACAGTAATTGGAGAACTTCCTCCAGCTTCCAGTGGCTCTGCATTAGCTGCTAATGTATGTAAAAAGATTACTGGACGTCTTACAAGTGCAATAGCAAAACAGGAAGATGTCTCTGTTCAGCTAGAAGCCTTGGATATTATGGCTGATATGTTGAGCAGGCAAGGAGGACTTCTTGTTAATTTCCATCCTTCAATTCTGACCTGTCTACTTCCCCAGTTGACCAGCCCTAGACTTGCAGTGAGGAAAAGAACCATTATCGCTCTTGGCCATCTGGTTATGAGCTGTGGAAATATAGTTTTTGTAGATCTTATTGAACATCTGTTGTCAGAGTTGTCCAAAAATGATTCTATGTCAACAACAAGAACCTACATACAATGTATTGCTGCTATTAGTAGGCAAGCTGGTCATAGAATAGGTGAATACCTTGAGAAGATAATTCCTTTGGTGGTAAAATTTTGCAATGTAGATGATGATGAATTAAGAGAGTACTGTATTCAAGCCTTTGAATCATTTGTAAGAAGATGTCCTAAGGAAGTATATCCTCATGTTTCTACCATTATAAATATTTGTCTTAAATATCTTACCTATGATCCAAATTATAATTACGATGATGAAGATGAAGATGAAAATGCAATGGATGCTGATGGTGGTGATGATGATGATCAAGGGAGTGATGATGAATACAGTGATGATGATGACATGAGTTGGAAAGTGAGACGTGCAGCTGCGAAGTGCTTGGATGCTGTAGTTAGCACAAGGCATGAAATGCTTCCAGAATTCTACAAGACCGTCTCTCCTGCACTAATATCCAGATTTAAAGAGCGTGAAGAGAATGTAAAGGCAGATGTTTTTCACGCATACCTTTCTCTTTTGAAGCAAACTCGTCCTGTACAAAGTTGGCTATGTGACCCTGATGCAATGGAGCAGGGAGAAACACCTTTAACAATGCTTCAGAGTCAGGTTCCCAACATTGTTAAAGCTCTTCACAAACAGATGAAAGAAAAAAGTGTGAAGACCCGACAGTGTTGTTTTAACATGTTAACTGAGCTGGTAAATGTATTACCTGGGGCCCTAACTCAACACATTCCTGTACTTGTACCAGGAATCATTTTCTCACTGAATGATAAATCAAGCTCATCGAATTTGAAGATCGATGCTTTGTCATGTCTATACGTAATCCTCTGTAACCATTCTCCTCAAGTCTTCCATCCTCACGTTCAGGCTTTGGTTCCTCCAGTGGTGGCTTGTGTTGGAGACCCATTTTACAAAATTACATCTGAAGCACTTCTTGTTACTCAACAGCTTGTCAAAGTAATTCGTCCTTTAGATCAGCCTTCCTCGTTTGATGCAACTCCTTATATCAAAGATCTATTTACCTGTACCATTAAGAGATTAAAAGCAGCTGACATTGATCAGGAAGTCAAGGAAAGGGCTATTTCCTGTATGGGACAAATTATTTGCAACCTTGGAGACAATTTGGGTTCTGACTTGCCTAATACACTTCAGATTTTCTTGGAGAGACTAAAGAATGAAATTACCAGGTTAACTACAGTAAAGGCATTGACACTGATTGCTGGGTCACCTTTGAAGATAGATTTGAGGCCTGTTCTGGGAGAAGGGGTTCCTATCCTTGCTTCATTTCTTAGAAAAAACCAGAGAGCTTTGAAACTGGGTACTCTTTCTGCCCTTGATATTCTAATAAAAAACTATAGTGACAGCTTGACAGCTGCCATGATTGATGCAGTTCTAGATGAGCTCCCACCTCTTATCAGCGAAAGTGATATGCATGTTTCACAAATGGCCATCAGTTTTCTTACCACTTTGGCAAAAGTATATCCCTCCTCCCTTTCAAAGATAAGTGGATCCATTCTCAATGAACTTATTGGACTTGTGAGATCACCCTTATTGCAGGGGGGAGCTCTTAGTGCCATGCTAGACTTTTTCCAAGCTCTGGTTGTCACTGGAACAAATAATTTAGGATACATGGATTTGTTGCGCATGCTGACTGGTCCAGTTTACTCTCAGAGCACAGCTCTTACTCATAAGCAGTCTTATTATTCCATTGCCAAATGTGTAGCTGCCCTTACTCGAGCATGCCCTAAAGAGGGACCAGCTGTAGTAGGTCAGTTTATTCAAGATGTCAAGAACTCAAGGTCTACAGATTCCATTCGTCTCTTAGCTCTACTTTCTCTTGGAGAAGTTGGGCATCATATTGACTTAAGTGGACAGTTGGAACTAAAATCTGTAATACTAGAAGCTTTCTCATCTCCTAGTGAAGAAGTCAAATCAGCTGCATCCTATGCATTAGGCAGCATTAGTGTGGGCAACCTTCCTGAATATCTACCGTTTGTCCTGCAAGAAATAACTAGTCAACCCAAAAGGCAGTATCTTTTACTTCATTCCTTGAAGGAAATTATTAGCTCTGCATCAGTGGTGGGCCTTAAACCATATGTTGAAAACATCTGGGCCTTATTACTAAAGCACTGTGAGTGTGCAGAGGAAGGAACCAGAAATGTTGTTGCTGAATGTCTAGGAAAACTCACTCTAATTGATCCAGAAACTCTCCTTCCACGGCTTAAGGGGTACTTGATATCAGGCTCATCATATGCCCGAAGCTCAGTGGTTACGGCTGTGAAATTTACAATTTCTGACCATCCACAACCTATTGATCCACTGTTAAAGAACTGCATAGGTGATTTCCTAAAAACTTTGGAAGACCCAGATTTGAATGTGAGAAGAGTAGCCTTGGTCACATTTAATTCAGCAGCACATAACAAGCCATCATTAATAAGGGATCTATTGGATACTGTTCTTCCACATCTTTACAATGAAACAAAAGTTAGAAAGGAGCTTATAAGAGAGGTAGAAATGGGTCCATTTAAACATACGGTTGATGATGGTCTGGATATTAGAAAGGCAGCATTTGAGTGTATGTACACACTTCTAGACAGTTGTCTTGATAGACTTGATATCTTTGAATTTCTAAATCATGTTGAAGATGGTTTGAAGGACCATTATGATATTAAGATGCTGACATTTTTAATGTTGGTGAGACTGTCTACCCTTTGTCCAAGTGCAGTACTGCAGAGGTTGGACCGACTTGTTGAGCCATTACGTGCAACATGTACAACTAAGGTAAAGGCAAACTCAGTAAAGCAGGAGTTTGAAAAACAAGATGAATTAAAGCGATCTGCCATGAGAGCAGTAGCAGCACTACTAACCATTCCAGAAGCAGAGAAGAGTCCACTGATGAGTGAATTCCAGTCACAGATCAGTTCTAACCCTGAGCTGGCGGCTATCTTTGAAAGTATCCAGAAAGATTCATCATCTACTAACTTGGAATCAATGGACACTAGTTAGgcggccgc

Amino Acid Sequence

MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLTWGFRFFETVNPENLYFQGGSRSEFKGLQSTSSLVAAAMASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPASSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINICLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAVVSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQGETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALLSLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTLIDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU23714
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: TEV not blunt; Parental 22313