cDNA Clone Triad1

• Synonyms: arIH2, ARI2, FLJ10938, FLJ33921
• Plasmid: pGEX_TEV- Triad1 L84A V86A W100A
• Parent Plasmid: pGEX TEV
• Expressed: GST-Triad1 (L84A,V86A,W100A)
• Genbank: NM_006321
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGTCAGTGGACATGAATAGCCAGGGGTCTGACAGCAATGAAGAGGACTATGACCCAAATTGTGAGGAAGAGGAAGAAGAAGAAGAAGACGACCCTGGGGACATAGAGGACTATTACGTGGGAGTAGCCAGCGATGTGGAGCAGCAGGGGGCTGATGCCTTTGATCCCGAGGAGTACCAGTTCACTTGCTTGACCTACAAGGAATCTGAGGGTGCCCTCAATGAGCACATGACCAGCTTAGCTTCTGTCgcAAAGgcATCTCATTCAGTTGCTAAACTTATATTAGTTAATTTCCACgcGCAAGTTTCAGAGATATTGGACAGATACAAGTCCAATTCTGCTCAACTGCTTGTTGAGGCTCGAGTTCAGCCTAATCCATCAAAACATGTTCCCACATCCCATCCCCCTCACCACTGTGCAGTGTGTATGCAGTTTGTGCGAAAGGAAAACCTACTCTCTCTGGCCTGTCAGCACCAGTTTTGCCGCAGCTGCTGGGAGCAGCACTGCTCAGTTCTCGTCAAGGACGGCGTGGGCGTGGGAGTCTCTTGCATGGCTCAGGACTGTCCACTCCGTACACCAGAGGACTTTGTGTTTCCATTGCTTCCCAATGAAGAATTGAGAGAGAAATACAGGCGCTACCTCTTCAGGGACTATGTGGAGAGTCATTACCAGCTCCAGCTGTGCCCTGGTGCAGACTGCCCCATGGTTATTCGGGTACAGGAGCCTAGAGCTCGCCGAGTACAGTGCAATCGGTGCAACGAGGTCTTCTGTTTCAAGTGTCGTCAGATGTATCACGCACCCACAGACTGTGCCACAATCCGGAAATGGCTCACGAAGTGTGCAGACGACTCTGAAACAGCCAACTACATTAGTGCTCACACTAAAGACTGTCCCAAGTGCAACATCTGCATTGAGAAGAATGGAGGCTGCAATCACATGCAATGCTCCAAATGTAAACACGACTTCTGCTGGATGTGTCTAGGAGATTGGAAGACTCATGGCAGTGAATACTATGAGTGCAGTCGTTACAAGGAGAATCCTGACATCGTGAACCAGAGCCAACAAGCCCAGGCGAGGGAAGCCCTCAAGAAGTACTTATTCTACTTTGAGAGGTGGGAAAACCACAATAAAAGCTTGCAGCTAGAGGCACAGACATACCAGCGGATTCACGAGAAGATTCAGGAGAGGGTCATGAACAATCTGGGGACATGGATCGACTGGCAGTACCTACAGAATGCTGCCAAGCTCTTGGCCAAGTGTCGATACACCCTGCAATACACCTACCCATATGCATATTACATGGAGTCCGGACCCAGGAAGAAGCTGTTTGAATACCAGCAGGCTCAGCTGGAGGCTGAGATCGAAAACCTCTCATGGAAAGTGGAGCGTGCAGACAGCTATGACAGAGGGGACTTGGAGAACCAGATGCATATAGCGGAGCAGCGGAGGAGAACCCTGCTGAAAGATTTCCATGACACCTAAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDENLYFQGGSMSVDMNSQGSDSNEEDYDPNCEEEEEEEEDDPGDIEDYYVGVASDVEQQGADAFDPEEYQFTCLTYKESEGALNEHMTSLASVAKASHSVAKLILVNFHAQVSEILDRYKSNSAQLLVEARVQPNPSKHVPTSHPPHHCAVCMQFVRKENLLSLACQHQFCRSCWEQHCSVLVKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKYRRYLFRDYVESHYQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCATIRKWLTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDFCWMCLGDWKTHGSEYYECSRYKENPDIVNQSQQAQAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEKIQERVMNNLGTWIDWQYLQNAAKLLAKCRYTLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDLENQMHIAEQRRRTLLKDFHDT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU23574
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: TEV cleavage; Parental: 20757