cDNA Clone ASTE1 (EAW79216)

• Plasmid: pcDNA5 FRT/TO Halo.TEV ASTE1 (EAW79216)
• Parent Plasmid: pcDNA5 FRT/TO Halo TEV
• Expressed: Halo.TEV (not blunt) ASTE1 (EAW79216)
• Genbank: EAW79216 (ENST00000514044)
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGGGTATCCGAGGACTAATGAGTTTTGTGGAAGATCATAGTAATGAGTTCTTCACTGATTTGAAGTTGCGGGACACAAAAATTGTCATTGATGGTTATGCTCTTTTCCACCGTCTTTGCTTCAGTTCAAACTTGGATCTCCGGTATGGAGGGGACTATGATTCTTTTGCAGATGTTGTACAAAAATTCTTTGAATCACTGTTTGCTTGTAATATATGCCCATATGTTGTATTAGATGGAGGATGTGACATTTCAGATAAAAAGCTTACAACTTTAAAGGATAGAGCTAGAGAGAAGATCCAGATGGCCCATTCCCTTTCTGTTGGTGGGAGTGGGTACGTATGTCCCTTACTCATCCGGGAAGTATTCATACAGGTTTTGATCAAGCTGCGGGTGTGTTTTGTCCAGTGCTTTTCAGAAGCAGATCGGGACATTATGACACTTGCTAACCATTGGAATTGCCCTGTGTTATCATCAGATAGTGACTTTTGCATTTTTGACCTGAAAACTGGGTTTTGCCCATTGAATAGCTTTCAGTGGAGAAATATGAACACTATTAAGGGCACACAAAACTATATCCCTGCCAAATGCTTTTCCCTTGATGCATTCTGCCATCACTTCAGCAATATGAATAAAGCTCTACTACCTCTCTTTGCGGTGCTATGTGGAAATGACCATGTTAATCTACCCATCATGGAGACATTCTTAAGTAAAGCGCGTCTTCCTCTTGGAGCTACCAGTTCTAAAGGGAGGAGACACCACCGAATCCTGGGACTTCTGAATTGGTTGTCTCATTTTGCCAACCCTACCGAAGCACTAGATAATGTTCTGAAATACCTCCCAAAAAAGGATCGAGAAAATGTTAAGGAACTTCTCTGCTGTTCCATGGAAGAATACCAACAGTCCCAGGTGAAGCTACAGGACTTCTTCCAGTGTGGTACTTATGTCTGTCCAGATGCCTTGAATCTTGGTTTACCAGAATGGGTATTAGTGGCTTTAGCTAAAGGCCAGCTATCTCCTTTCATCAGTGATGCTTTGGTCCTAAGACGGACCATTCTTCCCACACAGGTGGAAAACATGCAGCAACCAAATGCCCACAGAATATCTCAGCCCATCAGGCAAATCATCTATGGGCTTCTTTTAAATGCCTCACCACATCTGGACAAGACATCCTGGAATGCATTGCCTCCTCAGCCTCTAGCTTTCAGTGAAGTGGAAAGGATTAATAAAAATATCAGAACCTCAATCATTGATGCAGTAGAACTGGCCAAGGATCATTCTGACTTAAGCAGATTGACTGAGCTCTCCTTGAGGAGGCGGCAGATGCTTCTGTTAGAAACCCTGAAGGTGAAACAGACCATTCTGGAGCCAATCCCTACTTCACTGAAGTTGCCCATTGCTGTCAGTTGCTACTGGTTGCAGCACACCGAGACCAAAGCAAAGCTACATCATCTACAATCCTTACTGCTCACAATGCTAGTGGGGCCCTTGATTGCCATAATCAACAGCCCTGGTAAGGAAGAGCTGCAGGAAGATGGTGCTAAGATGTTGTATGCAGAGTTCCAAAGAGTGAAGGCGCAGACACGGCTGGGCACAAGACTGGACTTAGACACAGCTCACATCTTCTGTCAGTGGCAGTCCTGTCTCCAGATGGGGATGTATCTCAACCAGCTGCTGTCCACTCCTCTCCCAGAGCCAGACCTAACTCGCCCCAGTGCCTTCAATACTTTGAATGAGGATATATGGACGCATACAAAATATGGTGGGACTTTACATGTTTCAAGACTGTACAGTGGAAGCCTGGTGCACGGACTATGCCAGCAACTGCTAGCATCGACCTCTGTAGAAAGTCTCCTGAGCATATGTCCTGAGGCTAAGCAACTTTATGAATATCTATTCAATGCCACAAGGTCATATGCCCCCGCTGAAATATTCCTACCAAAAGGTAGATCAAATTCAAAAAAAAAAAGGCAGAAGAAACAGAATACCAGCTGTTCTAAGAACAGAGGGAGAACCACTGCACACACCAAGTGTTGGTATGAGGGAAACAACCGGTTTGGGTTGTTAATGGTTGAAAACTTAGAGGAACATAGTGAGGCCTCCAACATTGAATAAGCGGCCGC

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRN
IIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQ
AFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPA
EAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGENL
YFQGGSMGIRGLMSFVEDHSNEFFTDLKLRDTKIVIDGYALFHRLCFSSN
LDLRYGGDYDSFADVVQKFFESLFACNICPYVVLDGGCDISDKKLTTLKD
RAREKIQMAHSLSVGGSGYVCPLLIREVFIQVLIKLRVCFVQCFSEADRD
IMTLANHWNCPVLSSDSDFCIFDLKTGFCPLNSFQWRNMNTIKGTQNYIP
AKCFSLDAFCHHFSNMNKALLPLFAVLCGNDHVNLPIMETFLSKARLPLG
ATSSKGRRHHRILGLLNWLSHFANPTEALDNVLKYLPKKDRENVKELLCC
SMEEYQQSQVKLQDFFQCGTYVCPDALNLGLPEWVLVALAKGQLSPFISD
ALVLRRTILPTQVENMQQPNAHRISQPIRQIIYGLLLNASPHLDKTSWNA
LPPQPLAFSEVERINKNIRTSIIDAVELAKDHSDLSRLTELSLRRRQMLL
LETLKVKQTILEPIPTSLKLPIAVSCYWLQHTETKAKLHHLQSLLLTMLV
GPLIAIINSPGKEELQEDGAKMLYAEFQRVKAQTRLGTRLDLDTAHIFCQ
WQSCLQMGMYLNQLLSTPLPEPDLTRPSAFNTLNEDIWTHTKYGGTLHVS
RLYSGSLVHGLCQQLLASTSVESLLSICPEAKQLYEYLFNATRSYAPAEI
FLPKGRSNSKKKRQKKQNTSCSKNRGRTTAHTKCWYEGNNRFGLLMVENL
EEHSEASNIE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45298
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee