cDNA Clone SLX4 (mouse)

• Synonyms: AI256635, AI426760, Btbd12, D16Bwg1016e
• Plasmid: pBABE Neo SLX4m C1351A L1352A C1536R
• Parent Plasmid: pBabeD neo
• Expressed: SLX4m C1351A L1352A C1536R
• Genbank: NM_177472.4
• Species: Mouse

Sequence of Insert

GAATTCATGGTTCCAGAGAGTGCTCCCAATGGCAACTCCCAGCCTCTTCC
TTCCTGTTTCACGACAACTGGTGTACCAAGTCCCTCCAAACCCAGAGTCT
CAGAGCTGGTTCTGCAACGGATGAAGCAGTTCAAGAGAGCAGACCCTGAG
CGCCTGAGACACGCCTCAGAAGAATCCCCCCAAAAGACTGCACTGGGAGA
CGATGTCCCAAGGAGCCCTCCAGAGGAGACAGTGGGAGAAAATGAGTATA
AACTTGATGCCACAGACAGTGATGCTGCAATGGCCCTGGCCTTACAGCAG
GAGTTTAGAAGGGAGGAAGCATCCTCACACCATGACAGCCTGGAGGAGAA
GGGGTTGTTCTTCTGTCAAATGTGTCAGAAAAACCTCTCAGCAATGAATG
TAACCCGGAGGGAGCAACATGTGAACAGATGCCTAGATGAGGCAGAGAAG
GCACAGAGACCTGCTTCACCTCGGATCCCTGATTGCCCAATATGTGGGAA
ACCGTTCCTCACCACCAAGAGCCGAATCAGTCACTTGAAGCAGTGTGCGG
TGAGGATGGAAGTGGGTCCCCAACTCCTGCTTCAGGCTGTGAGGCTACAG
ACAGCTCAGCCAGAGGTGGATGGCAGCCCTCAGGTACCCAGTTTCAGCAA
CAACGTTGGAGGTTTGAAACGGAAAGGAGTCACCACCAAAAGGGAGCCAA
GGAGGAGGAAAGTCAATAAGCCTGAGGCTCCGTCTGAAGATCTGTTGGTG
GCCATGGCTCTGTCTCGCTCTGAGGTGGAGCACTGTCCAGTTGTGCCCCC
GCTCAGACTGGAAAATGCTTTTTCTGAGAAGATAAGGCTAGGAGCAGAGA
AGAAAAGTCGCAAGAAGAGACCCCCGGTGTGTCCCCCACAGTTGGTAACC
CAAGACTCTGAGACCACAGGTAGACAGATTGAAGATCGTGTTGCCCAGCT
TCTGTCAGAGGAAGCAGAACTATCTTGCACCCCACCACTTCTTGCCAGCA
AGATTTCTAAGGAAGAATTGGAACCTGCAGGCTGGCGAGCACGGCTGCCT
GAAGGGAAGCGGAACTTTCTGTGGGAACTCAGCGCTCTCACAGGAGCCTG
GGCTGAGGAGTCCTTCTACACAGTGGGACTGTTTCCTCCAATTGTGTCCC
AATGCCCTTCCAAGGAACCCCAGCTACCATTGGAGCTGCCCAAGCAAGGA
GAGCCGAGTCCACGGCGGCCACCTGCTTCCCAGAGCAGCCTTCCTGTGAG
CCACAGCCCCAAGATCCGGCTGCTGTCCTCCAGCCAGAGGGAGCGTCAAG
CCCTTCAGGACCTTGTGGACCTGGCAGTGGAAGGGCTGAGCTCTAGCCCA
CAGCCCGGGAGTCGGGGCGTACCCACAGGGTTGGACCTGGTACCTAGCAG
CCTTCCGCTGACGGGGTTTGTCCTTCCATGTAAGAAGACCCTGAAGAAGG
ATGACAGTGCCTCACTCTCTCTTGGGTTATTGGTGACAGACTTTGGGGCC
ATGGTCAATAATCCACACCTGAGTGATGTCCAGTTCCAGCTTGACAGTGG
AGAGGTGCTTTATGCACACAAGTTTGTTCTTTATGCGAGATGCCCACTTC
TTATTCAGTATGTAAGCACTGAAAGCTTTTCTTCTGAAGAGGACGGGGAT
CTGACCCAGCGTGCTCTGCTGAGTGATGTGAGCTCGGAGGCCGCCCATGC
ATTTCTGAACTACCTCTACATGGCAGACACTGACATGCCTCCCAGCCTGG
TTCCGGATCTGCGTTCCCTGGCCCTAAGGTTTGGTGTGAGTGACCTTGTT
CAGCTGTGTGAACAAGTGCCCGCCGTGGTGGACTTAGAGGGAGAACAGCC
AGAAGAGACCAGTGAGGACTGTGAAAGCCGAGCAGAGACTTTCCTGGAAC
TCCTGAGGTCGGTGTGGGTAGACAATGAGGAGGAAGTGGAGACTCTGCTG
AAACCTGAGCTCTGTGAAGAAGAGAGAGAAAGAGTGAATGAAGCAGAAAT
GGAAGAGATATATGAATTTGCTGCTACGCAGAGGAAGCTTCTCCAGTGGG
GGAGAGCAGCAGACCCAGATGGGAGCACTAACCCGCATGGGGAAGACGGT
GCAGTTTCTGAGCCTTCCCTGGCAGGTGTCCAGAGTAACAGACAGCTAGA
AAATACAGAGCACATGGAGTCATCTGGGTTAGAAAAAGAAGAGGCACTAG
CCAGCTGGGAACAAGAAGGACATTCCACTCCACTGCAGGACCAGTGCCCA
GACTGGGCAGGGAAAGCAGAGGCCCAGGATGCATTGGGGGAGGCAACTGA
CGACCCCAGCTTCTGCAGCCGCCACAGGAGGGGGAAAGAGTGCTTGCCTT
TGCACCCAAACAAGGCCCATGGCTGCAAACAGCCCCTTCCATCAAATCCA
AGAGTGTCATCTGAACTGTCACAAATAACAGTTGATCATGAAGAGCAGAG
TGACCATGTCAGGGAGACGCAGGCAGACATGGCCCAGGCACCTACTCCAC
ACTCCTGCAGCCTTGTGTCACAGTCTTCTGTGGATGGAAGTCCCAGCCAG
TCATGGCTTCACCTATACCACACTAGTCATTTGTCCCCCTCAGTGTCCCA
GTCACACAGTAGCATTTCCAGGGTGGCATCCCCAAGATCACTGTCTCCAA
CTACACCAACTAAGCAGAGGAGGGGCAGTAACATTGTCACATTACGGAAA
GATGCTGGCCACCACAGAGGCCAACAAAGTAGCCCCATAGCGGGACACAG
AAATAGGGGCATCCTGATTTCCCCAGCAAAGTCTCCACCCATTGACCTCA
CACAGTCAGTACCCGAGCCCCTGAGCCCCAGGGCCCAGGATCCTCTGCAT
TTCGTGAAAAAGGAGGATGAGGTCATCCTGTTACTGGACTCAGATGAGGA
GCTGGAGCACACCAAGACAGAGTCAGTTTCTAAAGATTCCCCAGAAGGAA
GGAAAGTTCCAGAATTTAGCCCCAGGTCCTCTGAACTGTTTTCAGTCATT
GATGTTGAAGAAGATCATGAACATTTCCAAAGCCCACTGAAGAGAGAGGC
CGGGCTACAGCACGGGGAGGAAGGACAGCTGGGAAACCAGTCTGCGCTGG
GCTGCAGAGACATCCCCTGGCTGCTCTGCAGCCAGAAAACCAGCCTGGAT
GAGGACAGTGCTACAGACACCTCTTGGCTGGTGCCTGCCACTCCAGGGGT
CAGCAGGAGTCGTGATTGCTCATCACAGACCCAAATCAAAAGCCTTAAGA
CCAGGATTCCGTCAGATGAGACTGCTCAGCAAACACCTAGACCTAACTTA
GAACGCAGGACCATGCTGGAAACAGCCCAGCAGTTCTCTGTGATCATGCC
ACACACACAGCCTATTACTCTAGGAGCCTTTGACAGCGGAAGACAGGCCT
ACAGAAGCCCTTCCCATCCCTACCCCAGGCACCACAGACTCTCTTCTTCA
CAGCCATCATGTCCTGGCCCTGATTTTACCAGGTGGTCCCAGAAATCTTC
CGCACCTAGACCATGCCTGCCAAATCTGCCTGCAGCTGATGATGTGGTAG
AAGTTGGGGACAGTGATGATGAGGTGGCTTCCCATCAAGGAAACAGCAGC
CCCGTGCTAGATGGTGACCCCCCAGGTCCCATGGGTGACTACTGTTGGAA
TGAGCCTCTCTCACCAATTCCAATTGACCACTTGAATCTGGAGCGGACCG
GCCCCCTGACCACAAGCAGTCCCAGCAGTCAGGTCCTGGAGGCTTTGCAC
AGTGATGACTGCCACTCCCCGGGACTTGGTACCACCCCCATCCGAGGAAG
CTGTGGTACGCTGAGAGAGTCTCAGGAACGGTCCTCACTGGCTGGCTCTC
CAGAGGCTCTGTGGGACGACTGGAATGAGGAGGAAGGACAGTCTCCAGAG
GCCCCACCTGTGGCTCAAATGCTGAGTACCAGAACTCGGAAGCCAGACAG
GCCAGAGACACCAAAAGGTGCTAACCAGAAAAAGAACTTGCCTCCCAAAG
TGCCTATAACTCCAATGCCAAGGTATTCGATCATGGAGACCCCGGTGCTG
AAGAAAGCAGCGGACAGGTTTGGAGTCCGTGCTCTGCCCAAACGCCAGAT
GGTTCTAAAACTGAAGGAGATTTTCCAGTACACTCACCAGACTCTGGAGT
CGGACTCTGAGGATGAGGTCCAGTCCCCCCAGATACCCGCAGAACTACCT
TGTCGCCAGGCATCTACCACTGAGACCTGCAACCCTTCAAGGTTGCCAAC
TGGGGAGCCTTCACATCCTGATGGTGACGCCCAGCTCCCAGCCTCCCAGG
AATCCATGGCTACCTCTGTGGACGGCAGCGACAACTCCTTTAGCTCTAAA
AGCTCCTCTGCTGAGTTTGGAGCTGCGTTTGAGTATTCAGATGAAGACAA
GGATGAGGAGGTCGGGGTCACTGCATCACAGGCCGCAATCCAGGCCGCAG
ACACAGAGGAGGCAGTGAGGCGCTATATCCGCTCTAAGCCTGCCCTGCAC
AGGCAGGTCCTGAGGTACCAGCCTGTGGAGCTGGCCGAGCTGCAGGCTGA
GCTGAAACAGAATGGCATCCCTGTGGCCATGGGCAAGCTGTCGGACATCC
TGGACGCTCAGCGCATCACCTTCACCACTGCTGCAGCCCGAAAGGAGAAA
CTCAAACACAAGAGACGGCAGCCCTCAGGCCGAAAGAAAAAGGATCAGAA
ATGAGCGGCCGC

Amino Acid Sequence

MVPESAPNGNSQPLPSCFTTTGVPSPSKPRVSELVLQRMKQFKRADPERL
RHASEESPQKTALGDDVPRSPPEETVGENEYKLDATDSDAAMALALQQEF
RREEASSHHDSLEEKGLFFCQMCQKNLSAMNVTRREQHVNRCLDEAEKAQ
RPASPRIPDCPICGKPFLTTKSRISHLKQCAVRMEVGPQLLLQAVRLQTA
QPEVDGSPQVPSFSNNVGGLKRKGVTTKREPRRRKVNKPEAPSEDLLVAM
ALSRSEVEHCPVVPPLRLENAFSEKIRLGAEKKSRKKRPPVCPPQLVTQD
SETTGRQIEDRVAQLLSEEAELSCTPPLLASKISKEELEPAGWRARLPEG
KRNFLWELSALTGAWAEESFYTVGLFPPIVSQCPSKEPQLPLELPKQGEP
SPRRPPASQSSLPVSHSPKIRLLSSSQRERQALQDLVDLAVEGLSSSPQP
GSRGVPTGLDLVPSSLPLTGFVLPCKKTLKKDDSASLSLGLLVTDFGAMV
NNPHLSDVQFQLDSGEVLYAHKFVLYARCPLLIQYVSTESFSSEEDGDLT
QRALLSDVSSEAAHAFLNYLYMADTDMPPSLVPDLRSLALRFGVSDLVQL
CEQVPAVVDLEGEQPEETSEDCESRAETFLELLRSVWVDNEEEVETLLKP
ELCEEERERVNEAEMEEIYEFAATQRKLLQWGRAADPDGSTNPHGEDGAV
SEPSLAGVQSNRQLENTEHMESSGLEKEEALASWEQEGHSTPLQDQCPDW
AGKAEAQDALGEATDDPSFCSRHRRGKECLPLHPNKAHGCKQPLPSNPRV
SSELSQITVDHEEQSDHVRETQADMAQAPTPHSCSLVSQSSVDGSPSQSW
LHLYHTSHLSPSVSQSHSSISRVASPRSLSPTTPTKQRRGSNIVTLRKDA
GHHRGQQSSPIAGHRNRGILISPAKSPPIDLTQSVPEPLSPRAQDPLHFV
KKEDEVILLLDSDEELEHTKTESVSKDSPEGRKVPEFSPRSSELFSVIDV
EEDHEHFQSPLKREAGLQHGEEGQLGNQSALGCRDIPWLLCSQKTSLDED
SATDTSWLVPATPGVSRSRDCSSQTQIKSLKTRIPSDETAQQTPRPNLER
RTMLETAQQFSVIMPHTQPITLGAFDSGRQAYRSPSHPYPRHHRLSSSQP
SCPGPDFTRWSQKSSAPRPCLPNLPAADDVVEVGDSDDEVASHQGNSSPV
LDGDPPGPMGDYCWNEPLSPIPIDHLNLERTGPLTTSSPSSQVLEALHSD
DCHSPGLGTTPIRGSCGTLRESQERSSLAGSPEALWDDWNEEEGQSPEAP
PVAQMLSTRTRKPDRPETPKGANQKKNLPPKVPITPMPRYSIMETPVLKK
AADRFGVRALPKRQMVLKLKEIFQYTHQTLESDSEDEVQSPQIPAELPCR
QASTTETCNPSRLPTGEPSHPDGDAQLPASQESMATSVDGSDNSFSSKSS
SAEFGAAFEYSDEDKDEEVGVTASQAAIQAADTEEAVRRYIRSKPALHRQ
VLRYQPVELAELQAELKQNGIPVAMGKLSDILDAQRITFTTAAARKEKLK
HKRRQPSGRKKKDQK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44379
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C1351A L1352A C1536R.; All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.