cDNA Clone MIRO1

• Synonyms: ARHT1, MIRO-1, RHOT1
• Plasmid: pcDNA5frtTO HA Miro1
• Parent Plasmid: pcDNA5D FRT/TO HA
• Expressed: HA-MIRO1
• Genbank: NM_018307.3
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGAAGAAAGACGTGCGAATTCTGCTGGTGGGAGAACCTAGAGTTGGGAAGACATCACTGATTATGTCTCTGGTCAGTGAAGAATTTCCAGAAGAGGTTCCTCCCCGGGCAGAAGAAATCACCATTCCAGCTGATGTCACCCCAGAGAGAGTTCCAACACACATTGTAGATTACTCAGAAGCAGAACAGAGTGATGAACAACTTCATCAAGAAATATCTCAGGCTAATGTCATCTGTATAGTGTATGCCGTTAACAACAAGCATTCTATTGATAAGGTAACAAGTCGATGGATTCCTCTCATAAATGAAAGAACAGACAAAGACAGCAGGCTGCCTTTAATATTGGTTGGGAACAAATCTGATCTGGTGGAATATAGTAGTATGGAGACCATCCTTCCTATTATGAACCAGTATACAGAAATAGAAACCTGTGTGGAGTGTTCAGCGAAAAACCTGAAGAACATATCAGAGCTCTTTTATTACGCACAGAAAGCTGTTCTTCATCCTACAGGGCCCCTGTACTGCCCAGAGGAGAAGGAGATGAAACCAGCTTGTATAAAAGCCCTTACTCGTATATTTAAAATATCTGATCAAGATAATGATGGTACTCTCAATGATGCTGAACTCAACTTCTTTCAGAGGATTTGTTTCAACACTCCATTAGCTCCTCAAGCTCTGGAGGATGTCAAGAATGTAGTCAGAAAACATATAAGTGATGGTGTGGCTGACAGTGGGTTGACCCTGAAAGGTTTTCTCTTTTTACACACACTTTTTATCCAGAGAGGGAGACACGAAACTACTTGGACTGTGCTTCGACGATTTGGTTATGATGATGACCTGGATTTGACACCTGAATATTTGTTCCCCCTGCTGAAAATACCTCCTGATTGCACTACTGAATTAAATCATCATGCATATTTATTTCTCCAAAGCACCTTTGACAAGCATGATTTGGATAGAGACTGTGCTTTGTCACCTGATGAGCTTAAAGATTTATTTAAAGTTTTCCCTTACATACCTTGGGGGCCAGATGTGAATAACACAGTTTGTACCAATGAAAGAGGCTGGATAACCTACCAGGGATTCCTTTCCCAGTGGACGCTCACGACTTATTTAGATGTACAGCGGTGCCTGGAATATTTGGGCTATCTAGGCTATTCAATATTGACTGAGCAAGAGTCTCAAGCTTCAGCTGTTACAGTGACAAGAGATAAAAAGATAGACCTGCAGAAAAAACAAACTCAAAGAAATGTGTTCAGATGTAATGTAATTGGAGTGAAAAACTGTGGGAAAAGTGGAGTTCTTCAGGCTCTTCTTGGAAGAAACTTAATGAGGCAGAAGAAAATTCGTGAAGATCATAAATCCTACTATGCGATTAACACTGTTTATGTATATGGACAAGAGAAATACTTGTTGTTGCATGATATCTCAGAATCGGAATTTCTAACTGAAGCTGAAATCATTTGTGATGTTGTATGCCTGGTATATGATGTCAGCAATCCCAAATCCTTTGAATACTGTGCCAGGATTTTTAAGCAACACTTTATGGACAGCAGAATACCTTGCTTAATCGTAGCTGCAAAGTCAGACCTGCATGAAGTTAAACAAGAATACAGTATTTCACCTACTGATTTCTGCAGGAAACACAAAATGCCTCCACCACAAGCCTTCACTTGCAATACTGCTGATGCCCCCAGTAAGGATATCTTTGTTAAATTGACAACAATGGCCATGTATCCGCACGTGACACAAGCTGACCTCAAGAGCTCCACGTTTTGGCTTCGAGCAAGTTTTGGTGCTACTGTTTTTGCAGTTTTGGGCTTTGCTATGTACAAAGCATTATTGAAACAGCGATGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAE
EITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKH
SIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQY
TEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKA
LTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHIS
DGVADSGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLF
PLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFKVFPY
IPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSI
LTEQESQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQAL
LGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEII
CDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEY
SISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHVTQADLK
SSTFWLRASFGATVFAVLGFAMYKALLKQR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44389
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Variant 3