cDNA Clone SLX4

• Synonyms: BTBD12
• Plasmid: pcDNA5 FRT/TO GFP SLX4 sprot K1575A
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP SLX4 sprot K1575A
• Genbank: NM_032444.2 GI:63252862 with changes: c3365t P1122L present in other published seqs, and silent changes t732t, c2007a, c2454t, t4500c. Also sprot changes.
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGAAACTGAGTGTGAATGAGGCTCAGCTAGGCTTCTACTTGGGTTCACTTTCTCATCTGTCTGCCTGTCCTGGGATTGACCCTCGCTCCTCTGAAGACCAGCCTGAAAGCCTTAAAACTGGTCAGATGATGGATGAGTCTGATGAGGACTTTAAAGAACTCTGCGCTAGCTTTTTCCAAAGGGTGAAAAAACATGGAATCAAGGAAGTGTCAGGAGAAAGGAAGACACAAAAGGCTGCCTCAAACGGCACTCAGATAAGAAGCAAATTGAAAAGGACCAAACAAACTGCTACCAAGACCAAAACCCTTCAAGGCCCTGCAGAGAAGAAACCTCCGTCTGGCAGCCAGGCCCCTAGGACTAAAAAGCAAAGGGTAACCAAATGGCAAGCAAGTGAACCGGCCCACTCTGTGAATGGGGAGGGGGGTGTGCTTGCCTCTGCTCCAGATCCACCTGTGCTCCGGGAAACAGCACAAAACACCCAGACGGGTAACCAGCAAGAACCATCGCCAAACCTTTCCAGAGAGAAAACCAGAGAGAATGTGCCCAACAGCGACTCCCAGCCTCCTCCTTCCTGTTTGACAACAGCAGTGCCAAGTCCCTCCAAACCCCGCACAGCACAATTGGTCCTACAGCGAATGCAGCAGTTCAAGAGAGCAGACCCCGAGCGTTTGAGACACGCTTCAGAAGAGTGCTCCCTCGAGGCTGCGCGGGAAGAAAATGTCCCAAAGGACCCTCAAGAGGAGATGATGGCGGGGAATGTGTATGGGCTTGGGCCCCCTGCCCCAGAGAGCGACGCTGCGGTGGCCTTGACCCTGCAGCAGGAGTTTGCACGGGTAGGAGCATCGGCACATGATGATAGCCTGGAGGAAAAGGGTTTGTTCTTCTGCCAGATTTGTCAAAAGAACCTCTCAGCCATGAACGTGACCCGAAGGGAACAGCATGTGAACAGGTGCTTGGATGAAGCTGAAAAGACACTAAGACCTTCTGTGCCTCAGATCCCTGAGTGCCCGATTTGTGGGAAACCGTTTCTTACCTTAAAGAGCAGAACCAGTCACTTGAAGCAGTGTGCTGTGAAGATGGAGGTTGGCCCCCAGCTCCTGCTTCAGGCTGTGCGGCTGCAGACAGCACAGCCTGAGGGTAGCAGCAGCCCACCCATGTTCAGCTTCAGTGATCACAGTAGAGGTCTGAAACGGAGAGGACCCACCAGCAAGAAGGAGCCACGGAAGAGGCGGAAGGTGGACGAGGCACCGTCCGAGGACCTGCTGGTGGCCATGGCTCTGTCCCGGTCGGAGATGGAGCCGGGTGCGGCTGTACCAGCGCTCAGGCTGGAAAGTGCCTTTTCTGAGAGGATAAGACCAGAAGCAGAGAATAAAAGTCGCAAGAAGAAACCCCCGGTATCCCCCCCATTGTTGTTAGTCCAGGACTCTGAAACCACAGGCCGACAGATAGAGGACCGTGTGGCCCTGCTCCTCTCTGAGGAAGTGGAATTGTCTAGCACGCCACCACTTCCTGCCAGCAGGATTTTAAAGGAAGGGTGGGAAAGAGCGGGCCAGTGTCCTCCTCCACCTGAACGCAAGCAGAGCTTTCTGTGGGAGGGCAGCGCACTGACTGGGGCCTGGGCCATGGAGGACTTCTACACGGCCAGGCTGGTCCCTCCTCTCGTGCCCCAGCGGCCTGCCCAGGGCCTTATGCAGGAGCCCGTGCCGCCTCTGGTGCCACCTGAGCACTCAGAGCTGAGCGAGCGAAGGTCACCCGCTCTCCACGGCACCCCCACTGCAGGCTGTGGCTCCAGGGGCCCGTCGCCTTCGGCCAGCCAGAGGGAGCACCAGGCCCTGCAGGACCTCGTGGACCTGGCGAGGGAGGGACTGAGCGCCAGCCCGTGGCCCGGCAGTGGGGGCCTGGCTGGCTCGGAAGGGACTGCAGGGTTGGACGTGGTGCCCGGCGGCCTTCCTCTGACTGGGTTTGTGGTGCCATCGCAGGACAAGCACCCGGACAGGGGCGGCCGAACCTTGCTCTCCCTCGGGCTGCTGGTTGCTGACTTTGGCGCCATGGTCAATAACCCACACCTGAGTGATGTCCAGTTTCAGACGGACAGCGGGGAGGTGCTTTACGCCCACAAGTTCGTGCTTTATGCCCGATGCCCGCTCCTCATCCAGTATGTGAACAATGAAGGCTTCTCCGCTGTAGAGGACGGGGTTCTGACCCAGCGTGTCCTGCTGGGTGACGTGAGCACCGAGGCCGCCCGCACGTTCCTGCACTATCTCTACACTGCGGACACTGGCCTTCCTCCTGGCCTTAGCTCTGAGCTGAGCTCCCTGGCCCACAGGTTTGGCGTGAGTGAGCTCGTTCACCTGTGCGAACAGGTGCCTATTGCCACTGACTCAGAGGGCAAACCATGGGAGGAGAAGGAAGCAGAGAATTGCGAAAGCAGGGCCGAGAATTTCCAGGAACTTTTGAGGTCAATGTGGGCAGATGAAGAGGAGGAAGCGGAGACTTTGTTGAAATCCAAGGACCACGAAGAAGATCAAGAAAACGTAAACGAGGCAGAGATGGAAGAAATTTATGAATTTGCAGCTACTCAGCGAAAGCTTCTCCAGGAAGAAAGGGCAGCGGGTGCCGGCGAGGACGCTGACTGGCTGGAGGGTGGCAGTCCGGTTTCTGGGCAACTCCTAGCAGGTGTCCAGGTGCAGAAACAGTGGGACAAGGTGGAGGAGATGGAGCCGTTGGAGCCAGGAAGAGATGAGGCCGCCACCACCTGGGAGAAGATGGGACAGTGCGCTCTCCCGCCACCCCAGGGCCAGCACTCAGGGGCACGGGGAGCAGAGGCCCCTGAGCAGGAGGCGCCAGAGGAGGCGCTTGGCCATTCCAGCTGCTCCAGCCCTTCCAGGGACTGCCAGGCAGAGAGAAAAGAAGGCTCTCTTCCGCACTCAGATGATGCCGGGGATTACGAACAGCTCTTCTCATCAACTCAGGGAGAGATCTCAGAGCCGTCCCAAATAACAAGTGAGCCCGAGGAACAAAGTGGCGCTGTCAGGGAAAGGGGGCTGGAGGTTTCTCATCGCCTGGCTCCCTGGCAGGCATCTCCACCGCACCCGTGCCGCTTCCTATTGGGGCCTCCCCAGGGCGGGAGTCCCCGCGGGTCTCATCACACAAGTGGGTCGTCCCTGTCAACACCCCGGTCCCGTGGCGGAACTTCCCAGGTGGGCTCCCCAACCTTGCTGTCTCCAGCTGTGCCATCAAAGCAGAAAAGGGACAGGAGCATCCTCACGCTGTCTAAAGAGCCAGGGCACCAGAAAGGCAAAGAGCGTCGGTCCGTGCTGGAGTGCAGAAATAAGGGGGTCCTGATGTTCCCAGAAAAATCTCTGTCTATTGACCTAACCCAGTCAAATCCTGACCATTCGAGCTCCAGATCTCAGAAATCTTCATCCAAACTGAACGAAGAAGATGAGGTCATCCTCTTACTGGACTCGGATGAGGAGCTGGAGCTAGAACAAACCAAAATGAAGTCCATTTCTAGTGATCCTCTGGAAGAAAAGAAAGCTCTAGAAATTAGCCCTAGGTCCTGTGAGCTGTTTTCCATCATTGATGTTGATGCAGATCAGGAACCTTCCCAGAGCCCACCAAGAAGCGAAGCTGTGCTGCAGCAGGAGGATGAGGGGGCGCTGCCGGAGAATCGGGGCTCTTTGGGCAGGAGAGGGGCTCCCTGGCTGTTCTGTGACCGTGAGAGCAGCCCCAGCGAGGCCAGCACCACAGACACCTCGTGGCTGGTGCCCGCCACCCCGCTGGCCAGCAGAAGCCGTGACTGTTCTTCCCAGACCCAAATCAGCAGCCTCAGGAGCGGGCTGGCCGTGCAGGCGGTGACTCAGCACACGCCCAGGGCCTCAGTAGGAAACAGGGAAGGGAACGAAGTCGCACAGAAGTTTTCTGTCATCAGGCCCCAGACACCACCGCCCCAGACACCGTCCTCATGCCTCACTCCCGTCTCTCCAGGAACTTCTGACGGCAGAAGGCAAGGCCACAGAAGCCCTTCCCGTCCCCACCCCGGGGGCCACCCGCACTCCTCTCCGCTGGCTCCACATCCCATCTCAGGGGACCGCGCCCACTTCAGCAGGCGGTTCCTGAAACACTCGCCGCCTGGGCCAAGCTTCCTGAACCAGACCCCAGCGGGTGAAGTGGTGGAAGTCGGAGACAGTGACGATGAGCAGGAGGTGGCCTCCCATCAGGCCAACAGAAGCCCCCCACTGGACAGTGACCCCCCAATTCCAATTGACGACTGCTGCTGGCACATGGAGCCCCTCTCGCCAATTCCCATTGACCACTGGAACCTGGAGCGGACCGGCCCCCTGAGCACCAGCAGCCCCAGCCGCAGGATGAACGAGGCCGCCGACAGCCGTGACTGTCGCTCCCCGGGACTCCTGGACACCACCCCCATCCGAGGAAGCTGCACTACCCAGAGGAAATTGCAAGAGAAGTCCTCGGGCGCGGGCTCCCTGGGGAACAGCAGGCCGAGCTTTCTGAATTCGGCTCTGTGGGACGTTTGGGACGGGGAAGAGCAGAGGCCTCCAGAGACCCCTCCTCCGGCCCAGATGCCAAGCGCTGGTGGAGCTCAGAAGCCCGAAGGGTTAGAGACACCCAAAGGTGCTAATCGGAAGAAGAACTTGCCCCCCAAAGTGCCCATAACGCCGATGCCACAGTATTCCATTATGGAGACGCCGGTGCTGGCCAAGGAACTGGATAGGTTTGGAGTCCGCCCTCTGCCTAAACGCCAGATGGTTCTGAAGCTGAAGGAGATATTCCAGTACACTCACCAGACCCTGGACTCAGACTCCGAGGACGAGAGCCAGTCCTCACAGCCGCTGTTGCAGGCGCCTCACTGCCAGACCCTCGCCTCCCAGACCTACAAGCCTTCAAGGGCAGGGGTCCATGCCCAGCAGGAGGCCACCACAGGACCTGGGGCCCATAGGCCCAAGGGACCTGCTAAGACCAAGGGCCCCCGACATCAAAGGAAGCATCATGAAAGCATCACACCCCCAAGCAGGTCGCCCACCAAGGAGGCACCTCCAGGCCTCAATGATGACGCCCAGATCCCAGCCTCTCAAGAATCCGTGGCCACCTCTGTGGATGGCAGTGACAGCTCCTTGAGCTCACAGAGTTCTTCCTCCTGTGAGTTTGGAGCGGCATTTGAGTCTGCAGGTGAAGAGGAGGGCGAGGGGGAGGTCAGTGCCTCGCAGGCAGCCGTGCAGGCGGCGGACACAGACGAGGCGCTGAGGTGCTACATCCGCTCCAAGCCGGCCCTGTACCAGAAGGTGCTGCTGTACCAGCCCTTTGAGCTGCGGGAGCTGCAGGCAGAGCTGAGGCAGAACGGCCTCCGTGTGTCCTCGCGCAGGCTGTTGGACTTCCTGGACACCCACTGTATCACCTTCACCACTGCCGCCACCCGCAGGGAGAAGCTCCAGGGCAGGAGGCGGCAGCCTCGGGGCAAGAAGAAGGTGGAGCGGAACTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMKLSVN
EAQLGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQMMDESDEDFKELCA
SFFQRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRTKQTATKTKTLQGP
AEKKPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGVLASAPDPPVLRET
AQNTQTGNQQEPSPNLSREKTRENVPNSDSQPPPSCLTTAVPSPSKPRTA
QLVLQRMQQFKRADPERLRHASEECSLEAAREENVPKDPQEEMMAGNVYG
LGPPAPESDAAVALTLQQEFARVGASAHDDSLEEKGLFFCQICQKNLSAM
NVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRTSHLKQC
AVKMEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDHSRGLKRRGPTSKKE
PRKRRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRLESAFSERIRPEAE
NKSRKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSEEVELSSTPPLPAS
RILKEGWERAGQCPPPPERKQSFLWEGSALTGAWAMEDFYTARLVPPLVP
QRPAQGLMQEPVPPLVPPEHSELSERRSPALHGTPTAGCGSRGPSPSASQ
REHQALQDLVDLAREGLSASPWPGSGGLAGSEGTAGLDVVPGGLPLTGFV
VPSQDKHPDRGGRTLLSLGLLVADFGAMVNNPHLSDVQFQTDSGEVLYAH
KFVLYARCPLLIQYVNNEGFSAVEDGVLTQRVLLGDVSTEAARTFLHYLY
TADTGLPPGLSSELSSLAHRFGVSELVHLCEQVPIATDSEGKPWEEKEAE
NCESRAENFQELLRSMWADEEEEAETLLKSKDHEEDQENVNEAEMEEIYE
FAATQRKLLQEERAAGAGEDADWLEGGSPVSGQLLAGVQVQKQWDKVEEM
EPLEPGRDEAATTWEKMGQCALPPPQGQHSGARGAEAPEQEAPEEALGHS
SCSSPSRDCQAERKEGSLPHSDDAGDYEQLFSSTQGEISEPSQITSEPEE
QSGAVRERGLEVSHRLAPWQASPPHPCRFLLGPPQGGSPRGSHHTSGSSL
STPRSRGGTSQVGSPTLLSPAVPSKQKRDRSILTLSKEPGHQKGKERRSV
LECRNKGVLMFPEKSLSIDLTQSNPDHSSSRSQKSSSKLNEEDEVILLLD
SDEELELEQTKMKSISSDPLEEKKALEISPRSCELFSIIDVDADQEPSQS
PPRSEAVLQQEDEGALPENRGSLGRRGAPWLFCDRESSPSEASTTDTSWL
VPATPLASRSRDCSSQTQISSLRSGLAVQAVTQHTPRASVGNREGNEVAQ
KFSVIRPQTPPPQTPSSCLTPVSPGTSDGRRQGHRSPSRPHPGGHPHSSP
LAPHPISGDRAHFSRRFLKHSPPGPSFLNQTPAGEVVEVGDSDDEQEVAS
HQANRSPPLDSDPPIPIDDCCWHMEPLSPIPIDHWNLERTGPLSTSSPSR
RMNEAADSRDCRSPGLLDTTPIRGSCTTQRKLQEKSSGAGSLGNSRPSFL
NSALWDVWDGEEQRPPETPPPAQMPSAGGAQKPEGLETPKGANRKKNLPP
KVPITPMPQYSIMETPVLAKELDRFGVRPLPKRQMVLKLKEIFQYTHQTL
DSDSEDESQSSQPLLQAPHCQTLASQTYKPSRAGVHAQQEATTGPGAHRP
KGPAKTKGPRHQRKHHESITPPSRSPTKEAPPGLNDDAQIPASQESVATS
VDGSDSSLSSQSSSSCEFGAAFESAGEEEGEGEVSASQAAVQAADTDEAL
RCYIRSKPALYQKVLLYQPFELRELQAELRQNGLRVSSRRLLDFLDTHCI
TFTTAATRREKLQGRRRQPRGKKKVERN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45411
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.