cDNA Clone FBXO7 mouse

• Synonyms: AI117679, AW260462, C85118
• Plasmid: pcDNA5frtTO GFP FBXO7m ko bam
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-FBXO7m
• Genbank: NM_153195.2
• Tag: GFP
• Species: Mouse

Sequence of Insert

GCGGCCGCGATGAAGCTGCGCGTGCGGCTTCAGAAGCGGACCCAGCCGCTCGAGGTGCCGGAGTCGGAGCCAACGCTCGGGCAGCTGCGCGCGCACCTCAGCCAGGTCCTGCTGCCCACGTTGGGGTTCAGTTCTGATACCCGATTTGCAATTACATTGAACAACAAGGATGCCCTCACTGGAGATGAAGAGACCTTGGCTTCATATGGGATTGTTTCTGGGGACTTGATATGTTTGGTTCTTGAAGATGACATGCCGGCACCTAACTTACCTTCATCTACAGATACAGAGCATTCCTCACTCCAGGATAATGACCAACCTTCTTTGGCTGCCACCCCAAGTCAGACTAACATCCCAGATGAACAAGGGACTGATTCATCCCAAGGACAGGCCACCCCATTTGATGCCTGGACTGATGACAGTATGGAAGGGCCTAGTCAAAATGTTGAAGCTGAGTCAATTCAGG-ATGCCATGAGTATGGAAGAGGTTTCTGGTTTCCAT-CCATTGGAACCAATGCTCTGTAATGAAACAGAGGATGGGCAGGTACCACATTCTCTAGAGACCCTGTACCAGTCAGCTGGCTGCTCCAACATCAGTGATGCCTTGATAGTGCTGGTGCATCTCCTGATGCTCGAGTCAGGCTACATTCCTCAGGGAACTGAAACCAAAGCAGTGACAATGCCTGAGAAGTGGAAGTCAAGTGGTGTATACAAACTGCAGTACACACATCCTCTCTGCGAGGGCGGCTTTGCCGTGCTTACCTGTGTGCCTTTGGGAAACTTAATCATTATAAATGCTACAATAAAAGTCAACGGTGGGATTAAAAACGTGAAGTCAGTGCAGCTGCAGCCAGGTTCCTACGTTGCTGCTGGAGTAGAACCAGGGGAAAGCGCAGCCAAAGTGTACAAAGATCTTAAGAAGCTCTCTCGTCTCTTCAAAGACCAGCTGGTGTACCCTCTTCTGGCTTTCACACGGCAAGTACTGAACCTTCCAGATGTGTTTGGGCTGGTTGTCCTTCCACTGGAGCTGAAACTACGCATCTTCCGACTTTTGGATGTTCATTCTGTCCTGGCCCTGTCTGCAGTCTGTCATGACCTCCTCATTGCGTCAAATGACCCACTGCTGTGGAGGTGTTTGTATCTGCGGGATTTTCGAGATGGCACTGTCAGAGGTCCTGACACAGACTGGAAAGAATTGTATAGAAAGAAGCACATACAAAGAAAAGAGGCTCAGAGGATGCGGCATGCAATGTTCCTACCATCAGCCCACCCTATCCCATTTTGTCCCATTCCCGTCTACCCCAGGGCCTACCTTCCCACCTCGCTGCTTCCTCCAGGAATCATTGGTGGTGAATATGATGAGAGGCCGATACTGCCTAGTGTTGGGGACCCAGTCACCTCACTTATCCCAAGGCCCGGGGAGCTTCCTGGTCAGTTCCGTCCACTCAGACCACGTTTTGACCCTGTTGACCCACTTCCAGGACCTCACTCCCTCTTGCCAGGAAGAGCTATCCCCAACAACAGATTTCCCTTCAGACCCGGCAGGGGTAGGTCAGCTGACAGCCGGCTGCCATTCCTGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSRKGTI
DAAAMKLRVRLQKRTQPLEVPESEPTLGQLRAHLSQVLLPTLGFSSDTRF
AITLNNKDALTGDEETLASYGIVSGDLICLVLEDDMPAPNLPSSTDTEHS
SLQDNDQPSLAATPSQTNIPDEQGTDSSQGQATPFDAWTDDSMEGPSQNV
EAESIQDAMSMEEVSGFHPLEPMLCNETEDGQVPHSLETLYQSAGCSNIS
DALIVLVHLLMLESGYIPQGTETKAVTMPEKWKSSGVYKLQYTHPLCEGG
FAVLTCVPLGNLIIINATIKVNGGIKNVKSVQLQPGSYVAAGVEPGESAA
KVYKDLKKLSRLFKDQLVYPLLAFTRQVLNLPDVFGLVVLPLELKLRIFR
LLDVHSVLALSAVCHDLLIASNDPLLWRCLYLRDFRDGTVRGPDTDWKEL
YRKKHIQRKEAQRMRHAMFLPSAHPIPFCPIPVYPRAYLPTSLLPPGIIG
GEYDERPILPSVGDPVTSLIPRPGELPGQFRPLRPRFDPVDPLPGPHSLL
PGRAIPNNRFPFRPGRGRSADSRLPFL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44504
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Knocked out internal BamHI, use this as template for downstream directional work with this gene.