cDNA Clone SPAK/MO25

• Plasmid: pFBDM MO25/GST-SPAK
• Parent Plasmid: pFBDM
• Expressed: GST-SPAK
• Genbank: AAC72238.1 GI:3851171
• Tag: GST
• Species: Human

Sequence of Insert

gaattcgcccttgatctgccaccatgtcccctatactaggttattggaaa
attaagggccttgtgcaacccactcgacttcttttggaatatcttgaaga
aaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaa
acaaaaagtttgaattgggtttggagtttcccaatcttccttattatatt
gatggtgatgttaaattaacacagtctatggccatcatacgttatatagc
tgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagattt
caatgcttgaaggagcggttttggatattagatacggtgtttcgagaatt
gcatatagtaaagactttgaaactctcaaagttgattttcttagcaagct
acctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatt
taaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctctt
gatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaatt
agtttgttttaaaaaacgtattgaagctatcccacaaattgataagtact
tgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacg
tttggtggtggcgaccatcctccaaaatcggatctggaagttctgttcca
ggggcccctgggatccATGGCGGAGCCGAGCGGCTCGCCCGTGCACGTCC
AGCTTCCCCAGCAGGCGGCCCCGGTGACAGCGGCGGCGGCGGCGGCCCCG
GCGGCCGCGACAGCAGCGCCGGCCCCGGCAGCTCCCGCGGCCCCGGCCCC
GGCCCCGGCCCCGGCCCCGGCGGCACAGGCTGTCGGCTGGCCCATCTGCA
GGGACGCGTACGAGCTGCAGGAGGTTATCGGCAGTGGAGCTACTGCTGTG
GTTCAGGCAGCCCTATGCAAACCCAGGCAAGAACGTGTAGCAATAAAACG
GATCAACTTGGAAAAATGCCAGACCAGTATGGATGAACTATTAAAAGAAA
TTCAAGCCATGAGTCAGTGCAGCCATCCCAACGTAGTGACCTATTACACC
TCTTTTGTGGTCAAAGATGAACTTTGGCTGGTCATGAAATTACTAAGTGG
AGGTTCAATGTTGGATATCATAAAATACATTGTCAACCGAGGAGAACACA
AGAATGGAGTTCTGGAAGAGGCAATAATAGCAACAATTCTTAAAGAGGTT
TTGGAAGGCTTAGACTATCTACACAGAAACGGTCAGATTCACAGGGATTT
GAAAGCTGGTAATATTCTTCTGGGTGAGGATGGTTCAGTACAAATAGCAG
ATTTTGGGGTAAGTGCGTTCCTAGCAACAGGGGGTGATGTTACCCGAAAT
AAAGTAAGAAAAACATTCGTTGGCACCCCATGTTGGATGGCTCCTGAAGT
CATGGAACAGGTGAGAGGCTATGACTTCAAGGCTGACATGTGGAGTTTTG
GAATAACTGCCATTGAATTAGCAACAGGAGCAGCGCCTTATCACAAATAT
CCTCCCATGAAAGTGTTAATGTTGACTTTGCAAAATGATCCACCCACTTT
GGAAACAGGGGTAGAGGATAAAGAAATGATGAAAAAGTACGGCAAGTCCT
TTAGAAAATTACTTTCACTGTGTCTTCAGAAAGATCCTTCCAAAAGGCCC
ACAGCAGCAGAACTTTTAAAATGCAAATTCTTCCAGAAAGCCAAGAACAG
AGAGTACCTGATTGAGAAGCTGCTTACAAGAACACCAGACATAGCCCAAA
GAGCCAAAAAGGTAAGAAGAGTTCCTGGGTCAAGTGGTCACCTTCATAAA
ACCGAAGACGGGGACTGGGAGTGGAGTGACGACGAGATGGATGAGAAGAG
CGAAGAAGGGAAAGCAGCTTTTTCTCAGGAAAAGTCACGAAGAGTAAAAG
AAGAAAATCCAGAGATTGCAGTGAGTGCCAGCACCATCCCCGAACAAATA
CAGTCCCTCTCTGTGCACGACTCTCAGGGCCCACCCAATGCTAATGAAGA
CTACAGAGAAGCTTCTTCTTGTGCCGTGAACCTCGTTTTGAGATTAAGAA
ACTCCAGAAAGGAACTTAATGACATACGATTTGAGTTTACTCCAGGAAGA
GATACAGCAGATGGTGTATCTCAGGAGCTCTTCTCTGCTGGCTTGGTGGA
TGGTCACGATGTAGTTATAGTGGCTGCTAATTTACAGAAGATTGTAGATG
ATCCCAAAGCTTTAAAAACATTGACATTTAAGTTGGCTTCTGGCTGTGAT
GGGTCGGAGATTCCTGATGAAGTGAAGCTGATTGGGTTTGCTCAGTTGAG
TGTCAGCTGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEPSGSPVHVQLPQQAAP
VTAAAAAAPAAATAAPAPAAPAAPAPAPAPAPAAQAVGWPICRDAYELQE
VIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCS
HPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEA
IIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFL
ATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELA
TGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLC
LQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRV
PGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAFSQEKSRRVKEENPEIAV
SASTIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLVLRLRNSRKELND
IRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTL
TFKLASGCDGSEIPDEVKLIGFAQLSVS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU45536
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloned MO25 from DU2051 Xho1/Kpn1 into DU41860 (pFBDM GST-SPAK). ; N.B. Xho1/Kpn1 insert from DU2051 has extra seq up and downstream of ORF so beware additional restriction sites if using to combine with other pFBDM clones.; ; All SPAK plasmids MUST be grown at 30 degrees C or less to prevent recombination.