cDNA Clone FBX07

• Synonyms: FBX, FBX7, PKPS, FBXO7, PARK15
• Plasmid: pET15b TEV HALO FBXO7 R378G 324-420
• Parent Plasmid: pET15b 6His TEV Halo 3C
• Expressed: 6xHIS-TEV-HALO-FBXO7 R378G 324-420
• Genbank: NM_012179.3
• Tag: 6His
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCGCCATGCTGAACCTACCAGATGTATTTGGGTTGGTCGTCCTCCCATTGGAACTGAAACTACGGATCTTCCGACTTCTGGATGTTCGTTCCGTCTTGTCTTTGTCTGCGGTTTGTCGTGACCTCTTTACTGCTTCAAATGACCCACTCCTGTGGAGGTTTTTATATCTGGGTGATTTTCGAGACAATACTGTCAGAGTTCAAGACACAGATTGGAAAGAACTGTACAGGAAGAGGCACATACAAAGAAAAGAATCCCCGAAAGGGCGGTTTGTGATGCTCCTGCCATCGTCAACTCACTAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSENLYFQGMAEIGTGFPFDPHYVEVLGE
RMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMG
KSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNP
ERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIE
GTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVA
LVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPG
LNLLQEDNPDLIGSEIARWLSTLEISGLEVLFQGPGSAMLNLPDVFGLVV
LPLELKLRIFRLLDVRSVLSLSAVCRDLFTASNDPLLWRFLYLGDFRDNT
VRVQDTDWKELYRKRHIQRKESPKGRFVMLLPSSTH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44678
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 324-420, R378G. Use TEV to cleave off 6xHIS, precission may be used downstream to remove the HALO tag if necessary.