cDNA Clone SPAK/MO25/WNK1

• Plasmid: pFBDM GST-SPAK/MO25/6His-WNK1 M1-E491
• Parent Plasmid: pFBDM
• Expressed: Untagged MO25
• Genbank: NM_016289 with silent change c243t
• Tag: untagged
• Species: Human

Sequence of Insert

CTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGCCCTTGAAACCATGCCGTTCCCGTTTGGGAAGTCTCACAAATCTCCAGCAGACATTGTGAAGAATCTGAAGGAGAGCATGGCTGTTCTGGAAAAGCAAGACATTTCTGATAAAAAAGCAGAAAAGGCTACAGAAGAAGTTTCCAAAAATCTGGTTGCCATGAAAGAAATTCTGTATGGCACAAATGAAAAAGAGCCTCAGACAGAAGCAGTAGCTCAACTTGCTCAAGAACTCTATAATAGTGGGCTCCTTAGTACCCTGGTAGCTGATTTACAGCTCATTGACTTTGAGGGCAAAAAAGACGTGGCTCAAATTTTCAACAATATTCTCAGAAGACAAATTGGTACGAGAACTCCTACTGTTGAATACATCTGCACCCAACAGAATATTTTGTTCATGTTATTGAAAGGGTATGAATCTCCAGAAATAGCTCTAAATTGTGGAATAATGTTAAGAGAATGCATCAGACATGAACCACTTGCAAAAATCATTTTGTGGTCGGAACAGTTTTATGATTTCTTCAGATATGTCGAAATGTCAACATTTGACATAGCTTCAGATGCATTTGCCACATTCAAGGATTTACTTACAAGACATAAATTGCTCAGTGCAGAATTTTTGGAACAGCATTATGATAGATTTTTCAGTGAATATGAGAAGTTACTTCATTCAGAAAATTATGTGACAAAAAGACAGTCACTGAAGCTTCTCGGTGAACTACTACTAGATAGACACAACTTCACAATTATGACAAAATACATCAGTAAACCTGAGAACCTCAAATTAATGATGAACCTGCTGCGAGACAAAAGTCGCAACATCCAGTTTGAGGCCTTTCACGTTTTTAAGGTGTTTGTAGCCAATCCTAACAAGACGCAGCCCATCCTAGACATCCTCCTCAAGAACCAGGCCAAACTCATAGAGTTCCTCAGCAAGTTTCAGAACGACAGGACGGAGGATGAGCAGTTTAACGACGAGAAGACCTATTTAGTTAAACAGATCAGGGATTTGAAGAGACCAGCTCAGCAAGAAGCTTAAGGTACC

Amino Acid Sequence

MPFPFGKSHKSPADIVKNLKESMAVLEKQDISDKKAEKATEEVSKNLVAM
KEILYGTNEKEPQTEAVAQLAQELYNSGLLSTLVADLQLIDFEGKKDVAQ
IFNNILRRQIGTRTPTVEYICTQQNILFMLLKGYESPEIALNCGIMLREC
IRHEPLAKIILWSEQFYDFFRYVEMSTFDIASDAFATFKDLLTRHKLLSA
EFLEQHYDRFFSEYEKLLHSENYVTKRQSLKLLGELLLDRHNFTIMTKYI
SKPENLKLMMNLLRDKSRNIQFEAFHVFKVFVANPNKTQPILDILLKNQA
KLIEFLSKFQNDRTEDEQFNDEKTYLVKQIRDLKRPAQQEA*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU45627
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 45 additional nucleotides between Xho1 and start site of MO25 (includes Not1 site). ; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.