cDNA Clone SLX4 (BTBD12)

• Plasmid: pET28a 6His-Halo.TEV 2X SLX4 (BTBD12) L270-G380
• Parent Plasmid: pET28a 6His Halo TEV
• Expressed: 6His-Halo-TEV 2X SLX4 (BTBD12) L270-G380
• Genbank: NM_032444.2
• Tag: 6His
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGTTGACCCTGCAGCAGGAGTTTGCACGGGTAGGAGCATCGGCACATGATGATAGCCTGGAGGAAAAGGGTTTGTTCTTCTGCCAGATTTGTCAAAAGAACCTCTCAGCCATGAACGTGACCCGAAGGGAACAGCATGTGAACAGGTGCTTGGATGAAGCTGAAAAGACACTAAGACCTTCTGTGCCTCAGATCCCTGAGTGCCCGATTTGTGGGAAACCGTTTCTTACCTTAAAGAGCAGAACCAGTCACTTGAAGCAGTGTGCTGTGAAGATGGAGGTTGGCCCCCAGCTCCTGCTTCAGGCTGTGCGGCTGCAGACAGCACAGCCTGAGGGTAGATCCATGTTGACCCTGCAGCAGGAGTTTGCACGGGTAGGAGCATCGGCACATGATGATAGCCTGGAGGAAAAGGGTTTGTTCTTCTGCCAGATTTGTCAAAAGAACCTCTCAGCCATGAACGTGACCCGAAGGGAACAGCATGTGAACAGGTGCTTGGATGAAGCTGAAAAGACACTAAGACCTTCTGTGCCTCAGATCCCTGAGTGCCCGATTTGTGGGAAACCGTTTCTTACCTTAAAGAGCAGAACCAGTCACTTGAAGCAGTGTGCTGTGAAGATGGAGGTTGGCCCCCAGCTCCTGCTTCAGGCTGTGCGGCTGCAGACAGCACAGCCTGAGGGTAGATCTTGACATCGAGTCGACGCTCGAGCGGCCGC

Amino Acid Sequence

MHHHHHHMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPT
SSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIE
ALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPE
FARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPF
LNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTP
GVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTL
EISGENLYFQGGSMLTLQQEFARVGASAHDDSLEEKGLFFCQICQKNLSA
MNVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRTSHLKQ
CAVKMEVGPQLLLQAVRLQTAQPEGRSMLTLQQEFARVGASAHDDSLEEK
GLFFCQICQKNLSAMNVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGK
PFLTLKSRTSHLKQCAVKMEVGPQLLLQAVRLQTAQPEGRS*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU45631
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.; Parental 45630