cDNA Clone CAND1

• Plasmid: pGEX-TEV-Cand1
• Parent Plasmid: pGEX6P1
• Expressed: GST-TEV-Cand1
• Genbank: Q86VP6
• Tag: GST

Sequence of Insert

ATGGCGAGCGCCTCGTACCACATTTCCAATTTGCTGGAAAAAATGACATCCAGCGACAAGGACTTTAGGTTTATGGCTACAAATGATTTGATGACGGAACTGCAGAAAGATTCCATCAAGTTGGATGATGATAGTGAAAGGAAAGTAGTGAAAATGATTTTGAAGTTATTGGAAGATAAAAATGGAGAGGTACAGAATTTAGCTGTCAAATGTCTTGGTCCTTTAGTGAGTAAAGTGAAAGAATACCAAGTAGAGACAATTGTAGATACCCTCTGCACTAACATGCTTTCTGATAAAGAACAACTTCGAGACATTTCAAGTATTGGTCTTAAAACAGTAATTGGAGAACTTCCTCCAGCTTCCAGTGGCTCTGCATTAGCTGCTAATGTATGTAAAAAGATTACTGGACGTCTTACAAGTGCAATAGCAAAACAGGAAGATGTCTCTGTTCAGCTAGAAGCCTTGGATATTATGGCTGATATGTTGAGCAGGCAAGGAGGACTTCTTGTTAATTTCCATCCTTCAATTCTGACCTGTCTACTTCCCCAGTTGACCAGCCCTAGACTTGCAGTGAGGAAAAGAACCATTATCGCTCTTGGCCATCTGGTTATGAGCTGTGGAAATATAGTTTTTGTAGATCTTATTGAACATCTGTTGTCAGAGTTGTCCAAAAATGATTCTATGTCAACAACAAGAACCTACATACAATGTATTGCTGCTATTAGTAGGCAAGCTGGTCATAGAATAGGTGAATACCTTGAGAAGATAATTCCTTTGGTGGTAAAATTTTGCAATGTAGATGATGATGAATTAAGAGAGTACTGTATTCAAGCCTTTGAATCATTTGTAAGAAGATGTCCTAAGGAAGTATATCCTCATGTTTCTACCATTATAAATATTTGTCTTAAATATCTTACCTATGATCCAAATTATAATTACGATGATGAAGATGAAGATGAAAATGCAATGGATGCTGATGGTGGTGATGATGATGATCAAGGGAGTGATGATGAATACAGTGATGATGATGACATGAGTTGGAAAGTGAGACGTGCAGCTGCGAAGTGCTTGGATGCTGTAGTTAGCACAAGGCATGAAATGCTTCCAGAATTCTACAAGACCGTCTCTCCTGCACTAATATCCAGATTTAAAGAGCGTGAAGAGAATGTAAAGGCAGATGTTTTTCACGCATACCTTTCTCTTTTGAAGCAAACTCGTCCTGTACAAAGTTGGCTATGTGACCCTGATGCAATGGAGCAGGGAGAAACACCTTTAACAATGCTTCAGAGTCAGGTTCCCAACATTGTTAAAGCTCTTCACAAACAGATGAAAGAAAAAAGTGTGAAGACCCGACAGTGTTGTTTTAACATGTTAACTGAGCTGGTAAATGTATTACCTGGGGCCCTAACTCAACACATTCCTGTACTTGTACCAGGAATCATTTTCTCACTGAATGATAAATCAAGCTCATCGAATTTGAAGATCGATGCTTTGTCATGTCTATACGTAATCCTCTGTAACCATTCTCCTCAAGTCTTCCATCCTCACGTTCAGGCTTTGGTTCCTCCAGTGGTGGCTTGTGTTGGAGACCCATTTTACAAAATTACATCTGAAGCACTTCTTGTTACTCAACAGCTTGTCAAAGTAATTCGTCCTTTAGATCAGCCTTCCTCGTTTGATGCAACTCCTTATATCAAAGATCTATTTACCTGTACCATTAAGAGATTAAAAGCAGCTGACATTGATCAGGAAGTCAAGGAAAGGGCTATTTCCTGTATGGGACAAATTATTTGCAACCTTGGAGACAATTTGGGTTCTGACTTGCCTAATACACTTCAGATTTTCTTGGAGAGACTAAAGAATGAAATTACCAGGTTAACTACAGTAAAGGCATTGACACTGATTGCTGGGTCACCTTTGAAGATAGATTTGAGGCCTGTTCTGGGAGAAGGGGTTCCTATCCTTGCTTCATTTCTTAGAAAAAACCAGAGAGCTTTGAAACTGGGTACTCTTTCTGCCCTTGATATTCTAATAAAAAACTATAGTGACAGCTTGACAGCTGCCATGATTGATGCAGTTCTAGATGAGCTCCCACCTCTTATCAGCGAAAGTGATATGCATGTTTCACAAATGGCCATCAGTTTTCTTACCACTTTGGCAAAAGTATATCCCTCCTCCCTTTCAAAGATAAGTGGATCCATTCTCAATGAACTTATTGGACTTGTGAGATCACCCTTATTGCAGGGGGGAGCTCTTAGTGCCATGCTAGACTTTTTCCAAGCTCTGGTTGTCACTGGAACAAATAATTTAGGATACATGGATTTGTTGCGCATGCTGACTGGTCCAGTTTACTCTCAGAGCACAGCTCTTACTCATAAGCAGTCTTATTATTCCATTGCCAAATGTGTAGCTGCCCTTACTCGAGCATGCCCTAAAGAGGGACCAGCTGTAGTAGGTCAGTTTATTCAAGATGTCAAGAACTCAAGGTCTACAGATTCCATTCGTCTCTTAGCTCTACTTTCTCTTGGAGAAGTTGGGCATCATATTGACTTAAGTGGACAGTTGGAACTAAAATCTGTAATACTAGAAGCTTTCTCATCTCCTAGTGAAGAAGTCAAATCAGCTGCATCCTATGCATTAGGCAGCATTAGTGTGGGCAACCTTCCTGAATATCTACCGTTTGTCCTGCAAGAAATAACTAGTCAACCCAAAAGGCAGTATCTTTTACTTCATTCCTTGAAGGAAATTATTAGCTCTGCATCAGTGGTGGGCCTTAAACCATATGTTGAAAACATCTGGGCCTTATTACTAAAGCACTGTGAGTGTGCAGAGGAAGGAACCAGAAATGTTGTTGCTGAATGTCTAGGAAAACTCACTCTAATTGATCCAGAAACTCTCCTTCCACGGCTTAAGGGGTACTTGATATCAGGCTCATCATATGCCCGAAGCTCAGTGGTTACGGCTGTGAAATTTACAATTTCTGACCATCCACAACCTATTGATCCACTGTTAAAGAACTGCATAGGTGATTTCCTAAAAACTTTGGAAGACCCAGATTTGAATGTGAGAAGAGTAGCCTTGGTCACATTTAATTCAGCAGCACATAACAAGCCATCATTAATAAGGGATCTATTGGATACTGTTCTTCCACATCTTTACAATGAAACAAAAGTTAGAAAGGAGCTTATAAGAGAGGTAGAAATGGGTCCATTTAAACATACGGTTGATGATGGTCTGGATATTAGAAAGGCAGCATTTGAGTGTATGTACACACTTCTAGACAGTTGTCTTGATAGACTTGATATCTTTGAATTTCTAAATCATGTTGAAGATGGTTTGAAGGACCATTATGATATTAAGATGCTGACATTTTTAATGTTGGTGAGACTGTCTACCCTTTGTCCAAGTGCAGTACTGCAGAGGTTGGACCGACTTGTTGAGCCATTACGTGCAACATGTACAACTAAGGTAAAGGCAAACTCAGTAAAGCAGGAGTTTGAAAAACAAGATGAATTAAAGCGATCTGCCATGAGAGCAGTAGCAGCACTACTAACCATTCCAGAAGCAGAGAAGAGTCCACTGATGAGTGAATTCCAGTCACAGATCAGTTCTAACCCTGAGCTGGCGGCTATCTTTGAAAGTATCCAGAAAGATTCATCATCTACTAACTTGGAATCAATGGACACTAGTTAG

Amino Acid Sequence

MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPASSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINICLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAVVSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQGETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALLSLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTLIDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTSZ

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU42771
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: there are no bases between SalI and ATG which needs to be adjusted according to target frame