cDNA Clone FBXO7 mouse

• Synonyms: AI117679, AW260462, C85118
• Plasmid: pET15b TEV HALO mFBXO7 324-498
• Parent Plasmid: pET15b 6His TEV Halo 3C
• Expressed: 6xHIS-HALO-mFBXO7 324-498
• Genbank: NM_153195.2
• Tag: 6His
• Tag: HALO
• Species: Mouse

Sequence of Insert

GGATCCGCCATGGTACTGAACCTTCCAGATGTGTTTGGGCTGGTTGTCCTTCCACTGGAGCTGAAACTACGCATCTTCCGACTTTTGGATGTTCATTCTGTCCTGGCCCTGTCTGCAGTCTGTCATGACCTCCTCATTGCGTCAAATGACCCACTGCTGTGGAGGTGTTTGTATCTGCGGGATTTTCGAGATGGCACTGTCAGAGGTCCTGACACAGACTGGAAAGAATTGTATAGAAAGAAGCACATACAAAGAAAAGAGGCTCAGAGGATGCGGCATGCAATGTTCCTACCATCAGCCCACCCTATCCCATTTTGTCCCATTCCCGTCTACCCCAGGGCCTACCTTCCCACCTCGCTGCTTCCTCCAGGAATCATTGGTGGTGAATATGATGAGAGGCCGATACTGCCTAGTGTTGGGGACCCAGTCACCTCACTTATCCCAAGGCCCGGGGAGCTTCCTGGTCAGTTCCGTCCACTCAGACCACGTTTTGACCCTGTTGACCCACTTCCAGGACCTCACTCCCTCTTGCCAGGATAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSENLYFQGMAEIGTGFPFDPHYVEVLGE
RMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMG
KSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNP
ERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIE
GTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVA
LVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPG
LNLLQEDNPDLIGSEIARWLSTLEISGLEVLFQGPLEVLFQGPGSAMVLN
LPDVFGLVVLPLELKLRIFRLLDVHSVLALSAVCHDLLIASNDPLLWRCL
YLRDFRDGTVRGPDTDWKELYRKKHIQRKEAQRMRHAMFLPSAHPIPFCP
IPVYPRAYLPTSLLPPGIIGGEYDERPILPSVGDPVTSLIPRPGELPGQF
RPLRPRFDPVDPLPGPHSLLPG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44720
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 324-498. Use TEV to cleave off 6xHIS, precission may be used downstream to remove the HALO tag if necessary.