cDNA Clone HECTD1

• Plasmid: pGEX6P HECTD1 F2073-N2610(end)
• Parent Plasmid: pGEX6P1
• Expressed: GST-HECTD1 F2073-N2610(end)
• Genbank: NM_015382.2 GI:118498336
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCTTTCTAATACCATTTGAAACTAGACAGCTTTATTTCACATGTACAGCATTTGGCGCCTCAAGAGCAATAGTATGGTTACAGAACCGACGTGAAGCCACTGTGGAACGAACGAGAACCACAAGCAGTGTTAGGCGAGATGACCCTGGAGAGTTTCGAGTTGGTCGTCTCAAGCATGAAAGAGTAAAAGTTCCACGTGGCGAGTCACTGATGGAATGGGCTGAGAATGTCATGCAAATACATGCAGATCGGAAATCAGTTCTTGAGGTTGAATTTTTAGGAGAAGAAGGAACTGGCTTGGGACCCACATTAGAGTTTTATGCTCTGGTGGCAGCAGAATTCCAGAGAACTGACTTGGGAGCTTGGCTTTGTGATGATAATTTTCCAGATGATGAATCTCGTCACGTTGATCTTGGAGGTGGATTGAAACCTCCTGGATATTATGTGCAGAGGTCATGTGGACTGTTCACAGCACCATTTCCACAGGATAGTGATGAGCTTGAAAGGATCACGAAACTGTTTCATTTCCTTGGAATTTTCTTGGCCAAATGCATTCAAGACAATAGACTTGTGGACTTACCTATTTCTAAACCTTTTTTTAAACTTATGTGTATGGGTGACATTAAAAGCAATATGAGTAAACTGATTTATGAGTCACGAGGTGATAGAGACTTACACTGTACTGAAAGTCAGTCTGAAGCTTCTACAGAAGAAGGTCATGATTCACTCTCGGTAGGAAGCTTTGAAGAGGATTCAAAATCAGAATTTATTCTTGATCCCCCTAAACCAAAACCCCCAGCTTGGTTTAATGGAATTTTGACTTGGGAAGACTTTGAATTAGTAAACCCACACAGAGCCAGATTTTTAAAAGAAATTAAAGACCTTGCTATCAAGAGGCGCCAAATTTTAAGCAACAAAGGTCTTTCTGAAGATGAGAAGAACACAAAATTACAGGAACTAGTGCTGAAGAATCCATCAGGTTCTGGGCCTCCACTTAGCATAGAGGATTTAGGTTTAAATTTCCAGTTTTGCCCTTCCTCAAGAATATATGGTTTTACAGCTGTGGATCTCAAGCCAAGTGGTGAAGATGAGATGATAACAATGGATAATGCAGAAGAATATGTGGATTTGATGTTTGACTTTTGTATGCATACGGGTATTCAGAAACAAATGGAAGCCTTTAGAGATGGGTTTAATAAAGTTTTTCCAATGGAGAAATTAAGTTCCTTCAGCCATGAAGAAGTCCAAATGATTCTTTGTGGAAACCAGTCACCATCCTGGGCAGCAGAGGATATTATCAATTACACTGAACCTAAGCTGGGTTATACACGTGACAGCCCTGGTTTCCTGAGGTTTGTGAGGGTTTTATGTGGCATGTCTTCTGATGAAAGGAAAGCATTCTTGCAGTTTACCACTGGTTGTTCAACTCTACCCCCAGGTGGACTGGCTAACCTGCATCCCAGGCTCACGGTTGTACGCAAGGTTGATGCTACTGATGCAAGCTATCCATCAGTCAATACATGTGTGCATTACCTTAAGTTGCCTGAATATTCTTCCGAGGAGATCATGAGAGAGCGCCTGCTAGCTGCTACAATGGAGAAAGGCTTTCATCTCAATTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSFLIPFETRQLYFTCTAFGA
SRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESL
MEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLG
AWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERI
TKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESR
GDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFN
GILTWEDFELVNPHRARFLKEIKDLAIKRRQILSNKGLSEDEKNTKLQEL
VLKNPSGSGPPLSIEDLGLNFQFCPSSRIYGFTAVDLKPSGEDEMITMDN
AEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILC
GNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQF
TTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSE
EIMRERLLAATMEKGFHLN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU45772
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee