cDNA Clone PINK1

• Plasmid: pMAL4C PINK1 123-581 G309D
• Parent Plasmid: pMAL4C
• Expressed: MBP PINK1 123-581 G309D
• Genbank: Q9BXM7
• Tag: MBP
• Species: Human

Sequence of Insert

ggatccTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAGCAA
GCCGGGGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTCGGCTGG
AGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTGTG
TATGAAGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGACAAAGAG
CACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAG
GGCAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATG
ATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAAT
GAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATG
GAGCAGTCACTTACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCCCT
CACCCCAACATCATCCGGGTTCTCCGCGCCTTCACCTCTTCCGTGCCGCT
GCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCTCC
ACCCTGAAGGCCTGGaCCATGGCCGGACGCTGTTCCTCGTTATGAAGAAC
TATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCG
CCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGG
TTCAACAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTTGTG
GAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTTTGGCTG
CTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCAGCTGGT
ACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCCACG
GCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGCCTG
GGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCTTCT
ACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAGGCTCAG
CTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTTGGTGAG
GGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAGTAGCCG
CAAATGTGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCCCTGAAG
AATCTGAAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATCGGCCGC
CACTTTGTTGGCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAACAAAAA
TGAAGATGCTCTTTCTGGCTAACCTGGAGTGTGAAACGCTCTGCCAGGCA
GCCCTCCTCCTCTGCTCATGGAGGGCAGCCCTGTGAgcggccgc

Amino Acid Sequence

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQ
VAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMF
NLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPT
FKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVIN
AASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
GSSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAV
YEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIKM
MWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAP
HPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLDHGRTLFLVMKN
YPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILV
ELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVST
ARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQ
LPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALK
NLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQA
ALLLCSWRAAL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46058
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: in pMal4c