cDNA Clone PINK1

• Synonyms: PTEN induced putative kinase 1
• Plasmid: pMAL4C PINK1 123-581 T185E S187E T257E S261E S284E
• Parent Plasmid: pMAL4C
• Expressed: MBP-PINK1 123-581 T185E S187E T257E S261E S284E
• Genbank: Q9BXM7, EAW94935
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCGCCACCTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAA
AAGCAAGCCGGGGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTA
GGCTGGAGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCT
GCTGTGTATGAAGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGGA
AAAGGAAACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAG
GAGAAGGGCAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATC
AAGATGATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAA
CACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGG
AGTATGGAGCAGTCGAATACAGAAAAGAAAAGAGAGGTCCCAAGCAACTA
GCCCCTCACCCCAACATCATCCGGGTTCTCCGCGCCTTCACCTCTGAAGT
GCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCAC
GCCTCCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATG
AAGAACTATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAG
CCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACC
ATCTGGTTCAACAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATC
CTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTT
TGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCA
GCTGGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTG
TCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGA
TGCCTGGGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATC
CCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAG
GCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTT
GGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAG
TAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCC
CTGAAGAATCTGAAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATC
GGCCGCCACTTTGTTGGCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAA
CAAAAATGAAGATGCTCTTTCTGGCTAACCTGGAGTGTGAAACGCTCTGC
CAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAGCCCTGTGATACAAGGA
CGACGATGACAAGTAGCGGCCGC

Amino Acid Sequence

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQ
VAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMF
NLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPT
FKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVIN
AASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
GSSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAV
YEATMPTLPQNLEVEKETGLLPGRGPGTSAPGEGQERAPGAPAFPLAIKM
MWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVEYRKEKRGPKQLAP
HPNIIRVLRAFTSEVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKN
YPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILV
ELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVST
ARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQ
LPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALK
NLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQA
ALLLCSWRAAL

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46014
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee