cDNA Clone PINK1

• Plasmid: pMAL4C PINk1 123-581 T185E
• Parent Plasmid: pMAL4C
• Expressed: MBP-human PINK1 (residues 123-end) [T185E]
• Genbank: Q9BXM7
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAGCAA
GCCGGGGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTCGGCTGG
AGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTGTG
TATGAAGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGGAAAAGAG
CACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAG
GGCAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATG
ATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAAT
GAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATG
GAGCAGTCACTTACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCCCT
CACCCCAACATCATCCGGGTTCTCCGCGCCTTCACCTCTTCCGTGCCGCT
GCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCTCC
ACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAAC
TATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCG
CCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGG
TTCAACAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTTGTG
GAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTTTGGCTG
CTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCAGCTGGT
ACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCCACG
GCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGCCTG
GGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCTTCT
ACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAGGCTCAG
CTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTTGGTGAG
GGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAGTAGCCG
CAAATGTGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCCCTGAAG
AATCTGAAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATCGGCCGC
CACTTTGTTGGCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAACAAAAA
TGAAGATGCTCTTTCTGGCTAACCTGGAGTGTGAAACGCTCTGCCAGGCA
GCCCTCCTCCTCTGCTCATGGAGGGCAGCCCTGTGAGCGGCCGC

Amino Acid Sequence

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQ
VAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMF
NLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPT
FKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVIN
AASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
GSSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAV
YEATMPTLPQNLEVEKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIKM
MWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAP
HPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKN
YPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILV
ELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVST
ARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQ
LPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALK
NLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQA
ALLLCSWRAAL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46042
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: in pMAL4C