cDNA Clone Mef2c

• Plasmid: pCMV5 FLAG MEF2C
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-MEF2C
• Genbank: EAW95965.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGGGAGAAAAAAGATTCAGATTACGAGGATTATGGATGAACGTAACAG
ACAGGTGACATTTACAAAGAGGAAATTTGGGTTGATGAAGAAGGCTTATG
AGCTGAGCGTGCTGTGTGACTGTGAGATTGCGCTGATCATCTTCAACAGC
ACCAACAAGCTGTTCCAGTATGCCAGCACCGACATGGACAAAGTGCTTCT
CAAGTACACGGAGTACAACGAGCCGCATGAGAGCCGGACAAACTCAGACA
TCGTGGAGACGTTGAGAAAGAAGGGCCTTAATGGCTGTGACAGCCCAGAC
CCCGATGCGGACGATTCCGTAGGTCACAGCCCTGAGTCTGAGGACAAGTA
CAGGAAAATTAACGAAGATATTGATCTAATGATCAGCAGGCAAAGATTGT
GTGCTGTTCCACCTCCCAACTTCGAGATGCCAGTCTCCATCCCAGTGTCC
AGCCACAACAGTTTGGTGTACAGCAACCCTGTCAGCTCACTGGGAAACCC
CAACCTATTGCCACTGGCTCACCCTTCTCTGCAGAGGAATAGTATGTCTC
CTGGTGTAACACATCGACCTCCAAGTGCAGGTAACACAGGTGGTCTGATG
GGTGGAGACCTCACGTCTGGTGCAGGCACCAGTGCAGGGAACGGGTATGG
CAATCCCCGAAACTCACCAGGTCTGCTGGTCTCACCTGGTAACTTGAACA
AGAATATGCAAGCAAAATCTCCTCCCCCAATGAATTTAGGAATGAATAAC
CGTAAACCAGATCTCCGAGTTCTTATTCCACCAGGCAGCAAGAATACGAT
GCCATCAGTGAATCAAAGGATAAATAACTCCCAGTCGGCTCAGTCATTGG
CTACCCCAGTGGTTTCCGTAGCAACTCCTACTTTACCAGGACAAGGAATG
GGAGGATATCCATCAGCCATTTCAACAACATATGGTACCGAGTACTCTCT
GAGTAGTGCAGACCTGTCATCTCTGTCTGGGTTTAACACCGCCAGCGCTC
TTCACCTTGGTTCAGTAACTGGCTGGCAACAGCAACACCTACATAACATG
CCACCATCTGCCCTCAGTCAGTTGGGAGCTTGCACTAGCACTCATTTATC
TCAGAGTTCAAATCTCTCCCTGCCTTCTACTCAAAGCCTCAACATCAAGT
CAGAACCTGTTTCTCCTCCTAGAGACCGTACCACCACCCCTTCGAGATAC
CCACAACACACGCGCCACGAGGCGGGGAGATCTCCTGTTGACAGCTTGAG
CAGCTGTAGCAGTTCGTACGACGGGAGCGACCGAGAGGATCACCGGAACG
AATTCCACTCCCCCATTGGACTCACCAGACCTTCGCCGGACGAAAGGGAA
AGTCCCTCAGTCAAGCGCATGCGACTTTCTGAAGGATGGGCAACATGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLC
DCEIALIIFNSTNKLFQYASTDMDKVLLKYTEYNEPHESRTNSDIVETLR
KKGLNGCDSPDPDADDSVGHSPESEDKYRKINEDIDLMISRQRLCAVPPP
NFEMPVSIPVSSHNSLVYSNPVSSLGNPNLLPLAHPSLQRNSMSPGVTHR
PPSAGNTGGLMGGDLTSGAGTSAGNGYGNPRNSPGLLVSPGNLNKNMQAK
SPPPMNLGMNNRKPDLRVLIPPGSKNTMPSVNQRINNSQSAQSLATPVVS
VATPTLPGQGMGGYPSAISTTYGTEYSLSSADLSSLSGFNTASALHLGSV
TGWQQQHLHNMPPSALSQLGACTSTHLSQSSNLSLPSTQSLNIKSEPVSP
PRDRTTTPSRYPQHTRHEAGRSPVDSLSSCSSSYDGSDREDHRNEFHSPI
GLTRPSPDERESPSVKRMRLSEGWAT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44870
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Isoform CRA_d. Missing amino acids 271-278 as compared to variant 1, otherwise in-frame.