cDNA Clone SPAK (mouse)

• Synonyms: Stk39, AW227544, AW556857, DCHT, RF005, Rnl5
• Plasmid: pGEX6P-1 mSPAK 64-138
• Parent Plasmid: pGEX6P1
• Expressed: GST-mSPAK 64-138
• Genbank: NM_016866.2
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCCAGGCGGTCGGCTGGCCCATCTGCAGGGACGCGTACGAGCTCCAGGAGGTTATCGGCAGCGGAGCAACTGCGGTGGTTCAGGCAGCCCTATGCAAACCCAGGCAAGAACGCGTAGCCATAAAGCGGATCAACTTGGAAAAATGCCAGACGAGTATGGATGAACTCCTGAAAGAAATTCAAGCCATGAGCCAATGCAGCCATCCCAACGTAGTGACTTATTACACCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLLEVLFQGPLGSQAVGWPICRD
AYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQ
AMSQCSHPNVVTYYT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44890
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 64-138. Note this construct is a backup, work will focus on 2-76 as the n-ter region for antibody production.; ; All SPAK plasmids MUST be grown at 30 degrees C or less to prevent recombination.