cDNA Clone SGK3

• Synonyms: CISK, SGK2, SGKL
• Plasmid: pcDNA5frtTO SGK3 R50A R90A C-GFP
• Parent Plasmid: pcDNA5D FRT/TO C-GFP
• Expressed: SGK3(R50/90A)-GFP
• Genbank: NM_013257.4
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGCAAAGAGATCACACCATGGACTACAAGGAAAGCTGCCCAAGTGTAAGCATTCCCAGCTCCGATGAACACAGAGAGAAAAAGAAGAGGTTTACTGTTTATAAAGTTCTGGTTTCAGTGGGAAGAAGTGAATGGTTTGTCTTCAGGGCATATGCAGAGTTTGATAAACTTTATAACACTTTAAAAAAACAGTTTCCTGCTATGGCCCTGAAGATTCCTGCCAAGAGAATATTTGGTGATAATTTTGATCCAGATTTTATTAAACAAGCACGAGCAGGACTAAACGAATTCATTCAGAACCTAGTTAGGTATCCAGAACTTTATAACCATCCAGATGTCAGAGCATTCCTTCAAATGGACAGTCCAAAACACCAGTCAGATCCATCTGAAGATGAGGATGAAAGAAGTTCTCAGAAGCTACACTCTACCTCACAGAACATCAACCTGGGACCGTCTGGAAATCCTCATGCCAAACCAACTGACTTTGATTTCTTAAAAGTTATTGGAAAAGGCAGCTTTGGCAAGGTTCTTCTTGCAAAACGGAAACTGGATGGAAAATTTTATGCTGTCAAAGTGTTACAGAAAAAAATAGTTCTCAACAGAAAAGAGCAAAAACATATTATGGCTGAACGTAATGTGCTCTTGAAAAATGTGAAACATCCGTTTTTGGTTGGATTGCATTATTCCTTCCAAACAACTGAAAAGCTTTATTTTGTTCTGGATTTTGTTAATGGAGGGGAGCTTTTTTTCCACTTACAAAGAGAACGGTCCTTTCCTGAGCACAGAGCTAGGTTTTACGCTGCTGAAATTGCTAGTGCATTGGGTTACTTACATTCCATCAAAATAGTATACAGAGACTTGAAACCAGAAAATATTCTTTTGGATTCAGTAGGACATGTTGTCTTAACAGATTTTGGGCTTTGTAAAGAAGGAATTGCTATTTCTGACACCACTACCACATTTTGTGGGACACCAGAGTATCTTGCACCTGAAGTAATTAGAAAACAGCCCTATGACAATACTGTAGATTGGTGGTGCCTTGGGGCTGTTCTGTATGAAATGCTGTATGGATTGCCTCCTTTTTATTGCCGAGATGTTGCTGAAATGTATGACAATATCCTTCACAAACCCCTAAGTTTGAGGCCAGGAGTGAGTCTTACAGCCTGGTCCATTCTGGAAGAACTCCTAGAAAAAGACAGGCAAAATCGACTTGGTGCCAAGGAAGACTTTCTTGAAATTCAGAATCATCCTTTTTTTGAATCACTCAGCTGGGCTGACCTTGTACAAAAGAAGATTCCACCACCATTTAATCCTAATGTGGCTGGACCAGATGATATCAGAAACTTTGACACAGCATTTACAGAAGAAACAGTTCCATATTCTGTGTGTGTATCTTCTGACTATTCTATAGTGAATGCCAGTGTATTGGAGGCAGATGATGCATTCGTTGGTTTCTCTTATGCACCTCCTTCAGAAGACTTATTTTTGGCGGCCGC

Amino Acid Sequence

MQRDHTMDYKESCPSVSIPSSDEHREKKKRFTVYKVLVSVGRSEWFVFRA
YAEFDKLYNTLKKQFPAMALKIPAKRIFGDNFDPDFIKQARAGLNEFIQN
LVRYPELYNHPDVRAFLQMDSPKHQSDPSEDEDERSSQKLHSTSQNINLG
PSGNPHAKPTDFDFLKVIGKGSFGKVLLAKRKLDGKFYAVKVLQKKIVLN
RKEQKHIMAERNVLLKNVKHPFLVGLHYSFQTTEKLYFVLDFVNGGELFF
HLQRERSFPEHRARFYAAEIASALGYLHSIKIVYRDLKPENILLDSVGHV
VLTDFGLCKEGIAISDTTTTFCGTPEYLAPEVIRKQPYDNTVDWWCLGAV
LYEMLYGLPPFYCRDVAEMYDNILHKPLSLRPGVSLTAWSILEELLEKDR
QNRLGAKEDFLEIQNHPFFESLSWADLVQKKIPPPFNPNVAGPDDIRNFD
TAFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSYAPPSEDLFLAAAM
VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTT
GKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFF
KDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNV
YIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHY
LSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44999
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: R50A R90A