cDNA Clone PINK1

• Plasmid: pMAL4C Pink1 123-581 T185E S187E T257E S284E L369P
• Parent Plasmid: pMAL4C
• Expressed: MBP-human PINK1/T185E/S187E/T257E/S284E/L369P (residues 123-end)
• Genbank: Q9BXM7
• Tag: MBP
• Species: Human

Sequence of Insert

CTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAGCAAGCCGG
GGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTAGGCTGGAGGAG
TATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTGTGTATGA
AGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGGAAAAGGAAACCG
GGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGGCAG
GAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATGATGTG
GAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGAGCC
AGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGAGCA
GTCGAATACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCCCTCACCC
CAACATCATCCGGGTTCTCCGCGCCTTCACCTCTGAAGTGCCGCTGCTGC
CAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCTCCACCCT
GAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAACTATCC
CTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCCTCG
CCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTTCAA
CAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCCTGTGGAGCT
GGACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTTTGGCTGCTGCC
TGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCAGCTGGTACGTG
GATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCCACGGCCCG
TCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGCCTGGGCAG
TGGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCTTCTACGGC
CAGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAGGCTCAGCTACC
TGCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTTGGTGAGGGCAC
TGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAGTAGCCGCAAAT
GTGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCCCTGAAGAATCT
GAAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATCGGCCGCCACTT
TGTTGGCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAACAAAAATGAAG
ATGCTCTTTCTGGCTAACCTGGAGTGTGAAACGCTCTGCCAGGCAGCCCT
CCTCCTCTGCTCATGGAGGGCAGCCCTGTGATACAAGGACGACGATGACA
AGTAGCGGCCGC

Amino Acid Sequence

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQ
VAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMF
NLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPT
FKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVIN
AASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
GSATSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSA
AVYEATMPTLPQNLEVEKETGLLPGRGPGTSAPGEGQERAPGAPAFPLAI
KMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVEYRKSKRGPKQL
APHPNIIRVLRAFTSEVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVM
KNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNI
PVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEV
STARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQE
AQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILA
LKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLC
QAALLLCSWRAAL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46382
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please introduce the following mutations: L369P into the existing clone DU43815 (MBP-human PINK1 T185E/S187E/T257E/S284E (residues 123-end) in pMAL4C vector)