cDNA Clone SGK3

• Synonyms: CISK, SGKL
• Plasmid: pMK-RQ SGK3 Nter GFP + polyA donor
• Parent Plasmid: pMK-RQ
• Genbank: ENSG00000104205
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCTAGTTATGTACTATACAGGATTGTAATCTGAATTGTTCAGCACACTTGACCAGTTCATCCTGATACAAGAAGTCTGAAAACCACACTTTGGTTACCTCCAACTAGATCTTTTCTAGGTGAGAGTTAGTCCCTAGCCTACGTGAACTGAGGTGTGTAAGTTCCTAGAATTTCCTAATAGTAAGAAAGGTAGATGGGCTGCAGATATTTGTGGCTTGTAGTAGGAACATAACAAACTAAATCTCTAACCTGGGCTGAGACTGATGAGCAAAAGTAGGTTCCTATCAGAATAAACGAAAATTAGTCCCATGCTTAAAGAATGACAGTCATAGTATTTTAAAGTTGTTTTGCTAATCACTTTTTTTTTTTTCTGTTGGTTAAGGTTGCATGATGGAATTTGAACATTACTTCAAGAGGTTTTGTATTTTGGATTAGTTAATTGGGTTTGTCCTCTGCTGACTGTTTCTTCGGATGCATTTTTTGGTGTGCTCTTGAGGGATTAAGCCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCAAAGGGACCATACTATGGATTACAAGGAAAGCTGCCCAAGTGTAAGCATTCCCAGCTCCGATGAACACAGAGAGAAAAAGAAGAGGTTTACTGTAAGTATTTAACATAAAGCATGTGGGAAAAAAGTTGAATGTAGAGAATATTTTCATTTCTGCTTTTCTTCTCCAACCTAGAACACCCCCTTCCCCATCTCCACATACCTAGATTTTACTTCTTTCACATGATCTGTTCTCCACAAAGTCTTCTGTGGCTACCGTAATGGAATAAATTTTCCTTTTTTAGTGTCTCTTTACTTATCTTCTTGGAGAATTGTATGAAACTATCTAATTAATCTTCTTAACAGAAAATGTGCATGTGCATACACATGGATCTATGCACACACTTATAATTTTGTGTGCACTGTCAGGAATTAATGGACTCCTGAAGTCATCGATTCCATATTAAGAACTACTGTCCTGAAATGTTTATAGTATCTCTCAGTTTTAAAATATTTAATTCAGTATTTTATTTTTTTCACCTTAAAGGCTGCGGCCGC

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU48167
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Designed to insert an in-frame GFP downstream of the SGK3 start codon in exon 2. Complete sequence of donor including homology arms shown as sequence of insert, contains several silent mutations to prevent Cas9 targeting the donor sequence. Successful recombination will knockout the SGK3 allele. Contains a BGH polyadenylation signal downstream of GFP to prevent degradation of the transcript via NMD.