cDNA Clone EVL18 (C. elegans)

• Synonyms: CDC45
• Plasmid: pET15b 6His 3C c. elegans EVL18 M1-T222
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His 3C c. elegans EVL18 M1-T222
• Genbank: NM_065355.4 GI:392894516
• Tag: 6His
• Species: Caenorhabditis elegans

Sequence of Insert

GGATCCATGATCATCGAAGACAACTTTCGGCAGGAATTCTACAATAAAATCAAGAAAGGCGATAAAAGTGTGGTTCTAATCGTTTCAGTCGACACGGATGCATTATGCACGACGATGATTTTGACGCATCTGCTACGCTGTGATGACATCCCATTCTCGATTCTTGCAGTGGAAGGATGGAGCTCGGTGGAGAAGATTTTTGCAGATTCACAAGAGCAAAAATGTCATTATATCCTAATCAATTGCGGAGCAACACGATCCCTGACACGACTACAAATCCCGCCGGCTTCAATAGCATTTGTCATTGATTCTCATCGACCATTTCACATTGAGAATGTCTATGAAAATGGTCAAATTCATTTATTGGCAAATCCAAGTGAAATGAGCGAATTGCAAATTCCTGATCTTGAATCTGTGATTCGAGAGGATTCTGACGATGAAGAAGATTCAGATGAAGATGAAGATCAAGAGTACATCTCATATGAACAACGAATGGAAAAGATTCGACGGAAAGCGATTAAGAGGGAAGAAATGCAAATATGGGAAAGGCAGAGGAGGACAATTCTCTGGAGATACTACGAATCGACGTGGTTCAGCTCGCCGAGCTGTGTGACTTTACTCGAGATGGCCGCAGAAATGAACAGAGTATCTGCTGAAACTATGTGGTTTACTTAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMIIEDNFRQEFYNKIKKGDKSVVLIVS
VDTDALCTTMILTHLLRCDDIPFSILAVEGWSSVEKIFADSQEQKCHYIL
INCGATRSLTRLQIPPASIAFVIDSHRPFHIENVYENGQIHLLANPSEMS
ELQIPDLESVIREDSDDEEDSDEDEDQEYISYEQRMEKIRRKAIKREEMQ
IWERQRRTILWRYYESTWFSSPSCVTLLEMAAEMNRVSAETMWFT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU49540
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make a construct to introduce 6His at the N-terminus of; C. elegant EVL-18 (1-222), for expression in E. coli. Please then pass this; to the protein expression and antibody team. We would like to make affinity; purified polyclonal antibodies for this protein in SHEEP.; I will be happy to supply either a cDNA library for C. elegans, or to help; make RNA if necessary.; ; Here is the sequence of the C. elegant N-terminus EVL-18 protein:; MIIEDNFRQEFYNKIKKGDKSVVLIVSVDTDALCTTMILTHLLRCDDIPF; SILAVEGWSSVEKIFADSQEQKCHYILINCGATRSLTRLQIPPASIAFVI; DSHRPFHIENVYENGQIHLLANPSEMSELQIPDLESVIREDSDDEEDSDE; DEDQEYISYEQRMEKIRRKAIKREEMQIWERQRRTILWRYYESTWFSSPS; CVTLLEMAAEMNRVSAETMWFT; ; Here is the sequence of C. elegant 5' fragment of evl-18 cDNA (without STOP; codon):; ATGATCATCGAAGACAACTTTCGGCAGGAATTCTACAATAAAATCAAGAA; AGGCGATAAAAGTGTGGTTCTAATCGTTTCAGTCGACACGGATGCATTAT; GCACGACGATGATTTTGACGCATCTGCTACGCTGTGATGACATCCCATTC; TCGATTCTTGCAGTGGAAGGATGGAGCTCGGTGGAGAAGATTTTTGCAGA; TTCACAAGAGCAAAAATGTCATTATATCCTAATCAATTGCGGAGCAACAC; GATCCCTGACACGACTACAAATCCCGCCGGCTTCAATAGCATTTGTCATT; GATTCTCATCGACCATTTCACATTGAGAATGTCTATGAAAATGGTCAAAT; TCATTTATTGGCAAATCCAAGTGAAATGAGCGAATTGCAAATTCCTGATC; TTGAATCTGTGATTCGAGAGGATTCTGACGATGAAGAAGATTCAGATGAA; GATGAAGATCAAGAGTACATCTCATATGAACAACGAATGGAAAAGATTCG; ACGGAAAGCGATTAAGAGGGAAGAAATGCAAATATGGGAAAGGCAGAGGA; GGACAATTCTCTGGAGATACTACGAATCGACGTGGTTCAGCTCGCCGAGC; TGTGTGACTTTACTCGAGATGGCCGCAGAAATGAACAGAGTATCTGCTGA; AACTATGTGGTTTACT