cDNA Clone Rap80/Cezanne

• Synonyms: UIMC1; X2HRIP110; ZA20D1; OTUD7B
• Plasmid: pcDNA5D FRT/TO GFP Rap80 60-124-NLS-Cezanne OTU (OTUD7B 124-438)
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP Rap80 60-124-NLS-Cezanne OTU (OTUD7B 124-438)
• Genbank: Rap80: NM_016290.4, Cezanne: NM_020205.3
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCACGAAGACAAAACAGTCGAATAGAGCAAAGTGTTTGGCCAAAAG
AAAAATCGCACAGATGACAGAAGAAGAACAGTTTGCTCTGGCTCTCAAAA
TGAGTGAGCAGGAAGCTAGGGAGGTGAACAGCCAGGAGGAGGAAGAAGAG
GAGCTCTTGAGGAAAGCCATTGCTGAAAGCCTGAATAGTTGCCGGCCTTC
TCCACCGAAGAAGAAGCGCAAGGTCAATGAGCACCCCCTGGAAATGCCCA
TCTGTGCCTTCCAGCTTCCAGATCTCACTGTATACAATGAAGACTTCCGC
AGCTTCATAGAGAGAGACCTCATTGAGCAGTCCATGCTGGTTGCCTTGGA
ACAGGCAGGGCGTTTGAACTGGTGGGTGAGTGTGGACCCCACCTCTCAGA
GGCTGCTTCCTTTGGCAACTACTGGAGATGGGAACTGCCTCCTGCATGCA
GCCTCCCTTGGAATGTGGGGTTTCCATGATCGGGACTTGATGCTGCGGAA
AGCTTTGTATGCACTGATGGAGAAGGGAGTTGAGAAGGAAGCGTTGAAAA
GGCGCTGGAGGTGGCAGCAGACACAGCAGAATAAAGAGTCAGGGCTGGTA
TACACAGAAGATGAATGGCAGAAGGAGTGGAATGAACTGATCAAGCTTGC
CTCAAGTGAACCCCGAATGCATCTAGGTACCAATGGAGCCAACTGTGGTG
GGGTGGAGAGTTCTGAGGAGCCTGTATATGAGAGCCTTGAAGAGTTTCAC
GTCTTTGTCCTTGCTCATGTGCTTAGGAGGCCCATAGTCGTCGTGGCAGA
CACCATGCTGAGGGACTCCGGAGGGGAAGCATTTGCCCCTATTCCCTTTG
GAGGAATCTATCTGCCTTTGGAGGTCCCAGCCAGCCAGTGTCACCGCTCC
CCTCTGGTGCTCGCCTATGATCAGGCCCACTTTTCTGCACTCGTGTCCAT
GGAGCAGAAGGAGAATACCAAGGAACAAGCTGTGATCCCACTTACAGATT
CAGAGTATAAGCTGCTGCCCTTGCACTTTGCTGTGGACCCTGGAAAGGGC
TGGGAGTGGGGCAAAGATGATAGTGACAATGTCCGATTGGCCAGTGTAAT
TCTGTCCCTAGAGGTCAAATTGCATCTGCTGCATAGCTACATGAATGTGA
AGTGGATTCCACTGTCCTCTTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSTKTKQS
NRAKCLAKRKIAQMTEEEQFALALKMSEQEAREVNSQEEEEEELLRKAIA
ESLNSCRPSPPKKKRKVNEHPLEMPICAFQLPDLTVYNEDFRSFIERDLI
EQSMLVALEQAGRLNWWVSVDPTSQRLLPLATTGDGNCLLHAASLGMWGF
HDRDLMLRKALYALMEKGVEKEALKRRWRWQQTQQNKESGLVYTEDEWQK
EWNELIKLASSEPRMHLGTNGANCGGVESSEEPVYESLEEFHVFVLAHVL
RRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQ
AHFSALVSMEQKENTKEQAVIPLTDSEYKLLPLHFAVDPGKGWEWGKDDS
DNVRLASVILSLEVKLHLLHSYMNVKWIPLSS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49525
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: (with silent changes to kill internal BamHI sites)