cDNA Clone Mph1 (yeast)

• Plasmid: pGADT7 5x(GA) Mph1
• Parent Plasmid: pGADT7
• Expressed: GAL4-AD HA 5x(GA)-yeast MphI
• Genbank: NM_001179524.1 GI:296145768
• Tag: GAL4 DNA-AD
• Species: Yeast

Sequence of Insert

CATATGGGAGCTGGTGCAGGCGCTGGAGCGGGTGCCATGGCTAGTGCAGATGATTACTTTAGTGATTTCGAAGATGACGAGCTGGATAAACTGTATGAAAAGGCTATCAATAAATCTGTGAAGGAAACTATAACGAGACGTGCTGTACCAGTTCAAAAAGACCTTCATGATAACGTTTTACCTGGGCAGAAAACTGTGTACGAAGAAATTCAAAGAGATGTCAGTTTTGGACCCACTCATCATGAGCTTGATTACGATGCTTTGTCGTTCTACGTGTACCCTACAAATTACGAGGTAAGGGATTATCAGTATACAATTGTTCACAAATCACTGTTTCAAAATACGTTATGCGCCATCCCAACGGGTATGGGTAAAACGTTCATTGCCAGTACAGTAATGTTAAATTATTTTCGTTGGACCAAAAAAGCCAAAATAATCTTTACTGCTCCAACAAGACCTTTGGTAGCCCAGCAAATTAAGGCATGCTTAGGTATAACAGGAATACCTTCGGATCAAACTGCTATATTACTCGATAAAAGCAGGAAAAATAGAGAAGAAATTTGGGCCAATAAGAGAGTTTTCTTTGCCACACCGCAAGTAGTGGAGAATGACTTGAAGAGGGGTGTATTGGATCCTAAGGATATTGTATGTCTTGTCATCGATGAAGCTCACAGAGCTACTGGCTCCTCTGCGTATACAAATGTGGTAAAATTCATAGATAGGTTCAATTCATCATACAGATTATTAGCATTGACCGCTACACCAGCCTCTGACCTGGAAGGTGTCCAAGAAGTGGTTAATAACTTAGATATTTCCAAGATTGAAATTAGGACTGAAGAAAGCATGGATATCGTGAAATATATGAAGAAAAGAAAAAAGGAAAAAATTGAAGTGCCCCTTTTGTTGGAAATTGAAGACATAATCGAACAATTAGGTATGGCCGTCAAACCAGTGCTTCAACAGGCAATTGAACTCGGTATATATGAGGAATGTGACCCTTCACAAATAAACGCCTTCAAAGCCATGCAACAAAGCCAAAAAATTATTGCTAACCCTACTATACCTGAAGGAATTAAGTGGAGGAACTTCTTTATTTTACAGTTATTGAACAATGTGGGTCAAATGCTAAAGAGATTAAAAATTTATGGAATAAGGACATTTTTCAATTACTTCCAGAATAAATGTACAGAATTCACTACGAAATATAATCTTAAAAAATCTACTAATAAAATTGCAGCTGAGTTTTATTACCATCCGATTTTGAAAAACATCAAAAATCAATGCGAAAATTATTTAAGTGATCCAAAGTTTGTCGGTCATGGGAAGTTACAATGTGTTAGAGATGAACTAATGGACTTTTTTCAGAAAAGGGGTTCCGACTCGAGGGTTATTATTTTTACAGAATTAAGAGAAAGCGCACTTGAAATTGTGAAGTTCATAGACTCGGTAGCTGACGATCAAATCAGGCCTCATATTTTTATTGGACAAGCTCGTGCAAAAGAAGGTTTTGATGAGGTCAAATACACAAGAAAACATGCGCCAAAGGGAAGGAAGAAGGTAGAGAGATTGCATAGGCAAGAGCAAGAGAAATTTTTGGAAGCTGAACGAACTAAGAGAGCGGCGAATGATAAACTTGAAAGAAGTGCCAGACGAACGGGGAGCTCTGAAGAGGCTCAGATTAGCGGAATGAACCAAAAAATGCAAAAAGAGGTTATCCATAATTTCAAAAAGGGTGAATATAATGTTCTCGTTTGTACATCAATTGGAGAAGAAGGTTTGGATATCGGAGAAGTTGATTTAATAATCTGTTATGATACTACAAGTAGTCCAATCAAAAATATTCAGCGTATGGGTAGAACAGGCAGAAAACGAGATGGTAAGATAGTACTACTGTTTAGCAGTAATGAATCATACAAATTCGAGCGTGCTATGGAAGACTATTCAACCCTGCAAGCGCTTATATCCAAGCAATGCATTGACTATAAAAAATCAGATCGGATAATCCCTGAAGACATTATTCCGGAATGTCACGAGACATTGATCACGATTAATGATGAAAACGAAATAATTAATGAAATGGAAGATGTTGACGAAGTTATTCGTTATGCGACTCAGTGTATGATGGGCAAAAAGGTGAAGCCAAAAAAAGCCATAACAAAGAAGAAGAGAGTTCAGGAAAATAAAAAACCGAAGAAGTTCTTTATGCCTGATAATGTTGAGACCAGTATTGTTAGTGCAAGCACTTTAATTAATAAGTTCTTGGTGAATGAAAGTGGCGGGAAACAACTAGTAACGAGTAATGAAAACCCAAGTAAGAAGCGGAAGATTTTTAAAGCTTTGGATAATCTGGAGAATGATTCAACCGAGGAAGCTTCCTCCTCATTAGAAACTGAAGATGAAGAAGTCAGTGATGATAATAATGTTTTCATTGCGGAAGGGCAGAATGGATGCCAAAAAGATTTGGAAACTGCCATTATTCGCACTGGCGAAAGTCTTACCACACTCAAGCCACTTCATAATTTTGAACGCCCTAATATGGCTTTATTTGTGAATGATTGTGGTCTTCCAACTAAAATCGAGAAAAATGTGAAAGACATCCGAGGAAATCAACACAACCTTGAAAAAGAAAAGAGCTGTACTGTTGACAAGAATAACATGGTATTGAGTTTGGACGATTGGAATTTTTTCAGAAACCGCTATATCCCAGAAGGTGTTTCCTTCGATGTGGAGCCCAATTTTGTACAATACACCAAAGGGGTAAAAGTTCCGCATTGTCACAAAGTTTCAAAAATCATTACGTTATTTAACGACGAGAGTAATGATAATAAGAAGAGGACAATTGATATGAACTATACTAAATGTTTGGCGAGAGGCATGTTACGAGACGAAAAAAAATTTGTAAAAGTTAACGATAAAAGTCAAGTCGATAACAACAGTGTCAACCATGATTCATCCCAATCTTTTACCTTATCGAACGCAGAATTAGATGACATACTGGGCTCAGATTCTGATTTTTGAAGATCT

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLI
TTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIFNTTHYRASAAME
YPYDVPDYAHMGAGAGAGAGAMASADDYFSDFEDDELDKLYEKAINKSVK
ETITRRAVPVQKDLHDNVLPGQKTVYEEIQRDVSFGPTHHELDYDALSFY
VYPTNYEVRDYQYTIVHKSLFQNTLCAIPTGMGKTFIASTVMLNYFRWTK
KAKIIFTAPTRPLVAQQIKACLGITGIPSDQTAILLDKSRKNREEIWANK
RVFFATPQVVENDLKRGVLDPKDIVCLVIDEAHRATGSSAYTNVVKFIDR
FNSSYRLLALTATPASDLEGVQEVVNNLDISKIEIRTEESMDIVKYMKKR
KKEKIEVPLLLEIEDIIEQLGMAVKPVLQQAIELGIYEECDPSQINAFKA
MQQSQKIIANPTIPEGIKWRNFFILQLLNNVGQMLKRLKIYGIRTFFNYF
QNKCTEFTTKYNLKKSTNKIAAEFYYHPILKNIKNQCENYLSDPKFVGHG
KLQCVRDELMDFFQKRGSDSRVIIFTELRESALEIVKFIDSVADDQIRPH
IFIGQARAKEGFDEVKYTRKHAPKGRKKVERLHRQEQEKFLEAERTKRAA
NDKLERSARRTGSSEEAQISGMNQKMQKEVIHNFKKGEYNVLVCTSIGEE
GLDIGEVDLIICYDTTSSPIKNIQRMGRTGRKRDGKIVLLFSSNESYKFE
RAMEDYSTLQALISKQCIDYKKSDRIIPEDIIPECHETLITINDENEIIN
EMEDVDEVIRYATQCMMGKKVKPKKAITKKKRVQENKKPKKFFMPDNVET
SIVSASTLINKFLVNESGGKQLVTSNENPSKKRKIFKALDNLENDSTEEA
SSSLETEDEEVSDDNNVFIAEGQNGCQKDLETAIIRTGESLTTLKPLHNF
ERPNMALFVNDCGLPTKIEKNVKDIRGNQHNLEKEKSCTVDKNNMVLSLD
DWNFFRNRYIPEGVSFDVEPNFVQYTKGVKVPHCHKVSKIITLFNDESND
NKKRTIDMNYTKCLARGMLRDEKKFVKVNDKSQVDNNSVNHDSSQSFTLS
NAELDDILGSDSDF*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU49600
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee