cDNA Clone DCUN1D5

• Plasmid: pU6 DCUN1D5 gRNA B
• Parent Plasmid: pU6 gRNA BamHI
• Expressed: DCUN1D5 gRNA B
• Genbank: ENSG00000137692
• Species: Human

Sequence of Insert

gAAGCAAGAAGTGCCTGGCT(TGG)

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48398
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Expresses DCUN1D5 guide RNA B, 19mer target sequence shown as sequence of insert with remaining 3 bases of PAM site in brackets (extra G added to aid expression). Targets exon 2, PAM site is situated on the sense strand. To be used in conjunction with DCUN1D5 guide RNA A, a ssDNA KO donor and Cas9 D10A nickase.