cDNA Clone PIAS1

• Synonyms: protein inhibitor of activated STAT 1, GBP, Ddxbp1, 2900068C24Rik
• Plasmid: pcDNA5-FRT/TO-EYFP-PIAS1 siRNA resistant
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: EYFP-PIAS1 siRNA resistant
• Genbank: BAC35902 Gene ID: 56469
• Tag: YFP
• Species: Mouse

Sequence of Insert

ggatccATGGCGGACAGTGCGGAACTAAAGCAAATGGTTATGAGCCTTAGAGTTTC
TGAACTCCAAGTACTGTTGGGCTACGCTGGGAGGAACAAGCACGGACGCA
AACACGAACTTCTTACAAAAGCCCTGCATTTGTTAAAGGCTGGCTGTAGT
CCTGCTGTACAAATGAAAATTAAAGAACTCTACAGGAGGCGGTTCCCTCA
GAAAATTATGACGCCTGCGGACTTGTCTATCCCCAACGTACATTCAAGTC
CTATGCCTCCGACTCTTTCTCCATCCACCATTCCACAGCTCACTTATGAT
GGCCACCCTGCATCATCCCCACTACTCCCTGTTTCTCTTCTGGGACCCAA
ACATGAACTGGAACTCCCACATCTCACGTCAGCGCTGCACCCAGTCCACC
CGGACATAAAGCTGCAGAAGCTACCATTCTATGACCTGTTGGATGAACTG
ATCAAGCCCACCAGTCTAGCTTCAGACAACAGCCAGCGCTTTCGGGAAAC
CTGTTTTGCATTTGCCTTGACACCACAACAGGTGCAGCAGATCAGCAGCT
CCATGGATATTTCTGGGACCAAATGTGACTTCACAGTGCAGGTCCAATTA
AGGTTTTGTTTATCAGAAACCAGTTGTCCACAAGAAGATCACTTCCCACC
CAACCTTTGTGTAAAAGTGAATACAAAACCTTGCAGCCTTCCAGGTTACC
TTCCACCTACTAAAAACGGTGTGGAACCAAAGCGACCTagtcgtcctATT
AATATCACCTCACTTGTCCGATTGTCCACGACAGTACCAAATACCATTGT
TGTTTCTTGGACTGCAGAAATTGGAAGAACCTATTCCATGGCAGTATATC
TTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGAGCAAAG
GGAATAagaaacccaGATCATTCTAGAGCTTTAATTAAAGAGAAGTTAAC
TGCAGATCCAGATAGTGAGATAGCTACTACCAGCCTACGGGTTTCGCTGC
TGTGTCCACTTGGGAAAATGCGACTGACAATCCCCTGTCGGGCACTTACC
TGCTCCCACCTTCAGTGTTTTGATGCAACTCTTTACATTCAAATGAATGA
GAAAAAACCAACATGGGTTTGTCCTGTCTGTGATAAGAAGGCCCCATATG
AACACCTTATTATTGACGGGTTGTTTATGGAAATTCTAAAGTACTGCACA
GACTGTGACGAGATACAGTTTAAGGAGGATGGCTCGTGGGCTCCAATGAG
GTCAAAGAAGGAGGTTCAAGAAGTCACTGCCTCCTACAATGGAGTTGATG
GTTGCTTGAGCTCCACATTGGAGCATCAGGTAGCGTCCCACAACCAGTCC
TCAAATAAAAACAAGAAAGTCGAGGTCATTGACCTAACCATTGACAGCTC
GTCAGATGAAGAGGAGGAAGAACCCCCTGCCAAGAGGACCTGTCCTTCCC
TGTCTCCTACGTCACCACTAAGTAATAAAGGCATTTTAAGTCTTCCTCAT
CAAGCCTCGCCTGTGTCCCGCACCCCAAGCCTTCCTGCTGTAGATACAAG
CTACATCAACACCTCCCTCATCCAGGACTACAGGCACCCCTTCCACATGA
CGCCTATGCCTTATGACTTACAAGGATTAGATTTCTTTCCTTTCTTATCA
GGAGACAATCAGCATTACAACACCTCCCTGCTAGCCGCAGCTGCAGCGGC
GGTCTCAGATGACCAGGACCTCCTGCACTCCTCCCGGTTTTTCCCGTATA
CCTCCTCGCAGATGTTTCTCGACCAGCTAAGTGCAGGAGGGAGCACATCT
CTGCCAGCCACCAACGGAAGCAGTAGCGGCAGCAACAGCAGCCTTGTGTC
TTCCAACAGTCTGAGAGAGAGCCATGGCCATGGTGTGGCCAGCAGGAGCA
GCGCAGACACAGCGTCCATCTTTGGCATCATACCAGACATTATCTCATTG
GACTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSMADSAELKQ
MVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKE
LYRRRFPQKIMTPADLSIPNVHSSPMPPTLSPSTIPQLTYDGHPASSPLL
PVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASD
NSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSC
PQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLS
TTVPNTIVVSWTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSR
ALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDA
TLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKE
DGSWAPMRSKKEVQEVTASYNGVDGCLSSTLEHQVASHNQSSNKNKKVEV
IDLTIDSSSDEEEEEPPAKRTCPSLSPTSPLSNKGILSLPHQASPVSRTP
SLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTS
LLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPATNGSSS
GSNSSLVSSNSLRESHGHGVASRSSADTASIFGIIPDIISLD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24546
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutations at S247 R248 P249 (agc cga cca → agt cgt cct ) and R303 N304 P305 (agg aat ccg → aga aac cca)