cDNA Clone PIAS4

• Plasmid: pcDNA5-FRT/TO-EYFP-PIAS4 siRNA resistant
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: EYFP-PIAS4 siRNA resistant
• Genbank: BC029874 NM_015897.2
• Tag: YFP
• Species: Human

Sequence of Insert

ggatccAAGATGGCGGCGGAGCTGGTGGAGGCCAAAAACATGGTGATGAGTTTTCGAGT
CTCCGACCTTCAGATGCTCCTGGGTTTCGTGGGCCGGAGTAAGAGTGGAC
TGAAGCACGAGCTCGTCACCAGGGCCCTCCAGCTGGTGCAGTTTGACTGT
AGCCCTGAGCTGTTCAAGAAGATCAAGGAGCTGTACGAGACCCGCTACGC
CAAGAAGAACTCGGAGCCTGCCCCACAGCCGCACCGGCCCCTGGACCCCC
TGACCATGCACTCCACCTACGACCGGGCCGGCGCTGTGCCCAGGACTCCG
CTGGCAGGCCCCAATATTGACTACCCCGTGCTCTACGGAAAGTACTTAAA
CGGACTGGGACGGTTGCCCGCCAAGACCCTCAAGCCAGAAGTCCGCCTGG
TGAAGCTGCCGTTCTTTAATATGCTGGATGAGCTGCTGAAGCCCACCGAA
ttggttcctCAGAACAACGAGAAGCTTCAGGAGAGCCCGTGCATCTTCGC
ATTGACGCCAAGACAGGTGGAGTTGATCCGGAACTCCAGGGAACTGCAGC
CCGGAGTTAAAGCCGTGCAGGTCGTCCTGAGAATCTGTTACTCAGACACC
AGCTGCCCTCAGGAGGACCAGTACCCGCCCAACATCGCTGTGAAGGTCAA
CCACAGCTACTGCTCCGTCCCGGGCTACTACCCCTCCAATAAGCCCGGGG
TGGAGCCCAAGAGGCCGTGCCGCCCCATCAACCTCACTCACCTCATGTAC
CTGTCCTCGGCCACCAACCGCATCACTGTCACCTGGGGGAACtatggtaa
aAGCTACTCGGTGGCCCTGTACCTGGTGCGGCAGCTGACCTCATCGGAGC
TGCTGCAGAGGCTGAAGACCATTGGGGTAAAGCACCCGGAGCTGTGCAAG
GCACTGGTCAAGGAGAAGCTGCGCCTTGATCCTGACAGCGAGATCGCCAC
CACCGGTGTGCGGGTGTCCCTCATCTGTCCGCTGGTGAAGATGCGGCTCT
CCGTGCCCTGCCGGGCAGAGACCTGCGCCCACCTGCAGTGCTTCGACGCC
GTCTTCTACCTGCAGATGAACGAGAAGAAGCCCACCTGGATGTGCCCCGT
GTGCGACAAGCCAGCCCCCTACGACCAGCTCATCATCGACGGGCTCCTCT
CGAAGATCCTGAGCGAGTGTGAGGACGCCGACGAGATCGAGTACCTGGTG
GACGGCTCGTGGTGCCCGATCCGCGCCGAAAAGGAGCGCAGCTGCAGCCC
GCAGGGCGCCATCCTCGTGCTGGGCCCCTCGGACGCCAATGGGCTCCTGC
CCGCCCCCAGCGTCAACGGGAGCGGTGCCCTGGGCAGCACGGGTGGCGGC
GGCCCGGTGGGCAGCATGGAGAATGGGAAGCCGGGCGCCGATGTGGTGGA
CCTCACGCTGGACAGCTCATCGTCCTCGGAGGATGAGGAGGAGGAGGAAG
AGGAGGAGGAAGACGAGGACGAAGAGGGGCCCCGGCCCAAGCGCCGCTGC
CCCTTCCAGAAGGGCCTGGTGCCGGCCTGCTGACCCCGGCCGCACACTCG
ACgcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSKMAAELVEA
KNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKI
KELYETRYAKKNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDY
PVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEK
LQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQY
PPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRI
TVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLR
LDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNE
KKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIR
AEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMEN
GKPGADVVDLTLDSSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVP
AC*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24547
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutations at L151 V152 P153 (tta gtc cca → ttg gtt cct ) and Y265 G266 K267 (tac ggc aag → tat ggt aaa). clone has an additional N-terminal lysine upstream from PIAS4 initiation. ORF stop located approx 20bp upstream of 3' Not1 cloning site.