cDNA Clone PIAS1

• Synonyms: protein inhibitor of activated STAT 1, GBP, Ddxbp1, 2900068C24Rik
• Plasmid: pcDNA5-FRT/TO-EYFP-PIAS1 (siRNA resistant) deletion SAP
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: EYFP-PIAS1 (siRNA resistant) delta Ala5-Arg64
• Genbank: BAC35902 Gene ID: 56469
• Tag: YFP
• Species: Mouse

Sequence of Insert

ggatccATGGCGGACAGTTTCCCTCAGAAAATTATGACGCCTGCGGACTTGTCTATCCCCAACGTACATTCAAGTCCTATGCCTCCGACTCTTTCTCCATCCACCATTCCACAGCTCACTTATGATGGCCACCCTGCATCATCCCCACTACTCCCTGTTTCTCTTCTGGGACCCAAACATGAACTGGAACTCCCACATCTCACGTCAGCGCTGCACCCAGTCCACCCGGACATAAAGCTGCAGAAGCTACCATTCTATGACCTGTTGGATGAACTGATCAAGCCCACCAGTCTAGCTTCAGACAACAGCCAGCGCTTTCGGGAAACCTGTTTTGCATTTGCCTTGACACCACAACAGGTGCAGCAGATCAGCAGCTCCATGGATATTTCTGGGACCAAATGTGACTTCACAGTGCAGGTCCAATTAAGGTTTTGTTTATCAGAAACCAGTTGTCCACAAGAAGATCACTTCCCACCCAACCTTTGTGTAAAAGTGAATACAAAACCTTGCAGCCTTCCAGGTTACCTTCCACCTACTAAAAACGGTGTGGAACCAAAGCGACCTAGTCGTCCTATTAATATCACCTCACTTGTCCGATTGTCCACGACAGTACCAAATACCATTGTTGTTTCTTGGACTGCAGAAATTGGAAGAACCTATTCCATGGCAGTATATCTTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGAGCAAAGGGAATAAGAAACCCAGATCATTCTAGAGCTTTAATTAAAGAGAAGTTAACTGCAGATCCAGATAGTGAGATAGCTACTACCAGCCTACGGGTTTCGCTGCTGTGTCCACTTGGGAAAATGCGACTGACAATCCCCTGTCGGGCACTTACCTGCTCCCACCTTCAGTGTTTTGATGCAACTCTTTACATTCAAATGAATGAGAAAAAACCAACATGGGTTTGTCCTGTCTGTGATAAGAAGGCCCCATATGAACACCTTATTATTGACGGGTTGTTTATGGAAATTCTAAAGTACTGCACAGACTGTGACGAGATACAGTTTAAGGAGGATGGCTCGTGGGCTCCAATGAGGTCAAAGAAGGAGGTTCAAGAAGTCACTGCCTCCTACAATGGAGTTGATGGTTGCTTGAGCTCCACATTGGAGCATCAGGTAGCGTCCCACAACCAGTCCTCAAATAAAAACAAGAAAGTCGAGGTCATTGACCTAACCATTGACAGCTCGTCAGATGAAGAGGAGGAAGAACCCCCTGCCAAGAGGACCTGTCCTTCCCTGTCTCCTACGTCACCACTAAGTAATAAAGGCATTTTAAGTCTTCCTCATCAAGCCTCGCCTGTGTCCCGCACCCCAAGCCTTCCTGCTGTAGATACAAGCTACATCAACACCTCCCTCATCCAGGACTACAGGCACCCCTTCCACATGACGCCTATGCCTTATGACTTACAAGGATTAGATTTCTTTCCTTTCTTATCAGGAGACAATCAGCATTACAACACCTCCCTGCTAGCCGCAGCTGCAGCGGCGGTCTCAGATGACCAGGACCTCCTGCACTCCTCCCGGTTTTTCCCGTATACCTCCTCGCAGATGTTTCTCGACCAGCTAAGTGCAGGAGGGAGCACATCTCTGCCAGCCACCAACGGAAGCAGTAGCGGCAGCAACAGCAGCCTTGTGTCTTCCAACAGTCTGAGAGAGAGCCATGGCCATGGTGTGGCCAGCAGGAGCAGCGCAGACACAGCGTCCATCTTTGGCATCATACCAGACATTATCTCATTGGACTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSMADSFPQKI
MTPADLSIPNVHSSPMPPTLSPSTIPQLTYDGHPASSPLLPVSLLGPKHE
LELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCF
AFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNL
CVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVS
WTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTAD
PDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKK
PTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGSWAPMRSK
KEVQEVTASYNGVDGCLSSTLEHQVASHNQSSNKNKKVEVIDLTIDSSSD
EEEEEPPAKRTCPSLSPTSPLSNKGILSLPHQASPVSRTPSLPAVDTSYI
NTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVS
DDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPATNGSSSGSNSSLVSSN
SLRESHGHGVASRSSADTASIFGIIPDIISLD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24568
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutations at S247 R248 P249 (agc cga cca → agt cgt cct ) and R303 N304 P305 (agg aat ccg → aga aac cca) plus SAP mutation (delat Ala5-Arg64)