cDNA Clone PIAS1

• Synonyms: protein inhibitor of activated STAT 1, GBP, Ddxbp1, 2900068C24Rik
• Plasmid: pcDNA5-FRT/TO-EYFP-PIAS1 (siRNA resistant) deletion SIM
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: EYFP-PIAS1 (siRNA resistant) delta V457-S466
• Genbank: BAC35902 Gene ID: 56469
• Tag: YFP
• Species: Mouse

Sequence of Insert

ggatccATGGCGGACAGTGCGGAACTAAAGCAAATGGTTATGAGCCTTAGAGTTTC
TGAACTCCAAGTACTGTTGGGCTACGCTGGGAGGAACAAGCACGGACGCA
AACACGAACTTCTTACAAAAGCCCTGCATTTGTTAAAGGCTGGCTGTAGT
CCTGCTGTACAAATGAAAATTAAAGAACTCTACAGGAGGCGGTTCCCTCA
GAAAATTATGACGCCTGCGGACTTGTCTATCCCCAACGTACATTCAAGTC
CTATGCCTCCGACTCTTTCTCCATCCACCATTCCACAGCTCACTTATGAT
GGCCACCCTGCATCATCCCCACTACTCCCTGTTTCTCTTCTGGGACCCAA
ACATGAACTGGAACTCCCACATCTCACGTCAGCGCTGCACCCAGTCCACC
CGGACATAAAGCTGCAGAAGCTACCATTCTATGACCTGTTGGATGAACTG
ATCAAGCCCACCAGTCTAGCTTCAGACAACAGCCAGCGCTTTCGGGAAAC
CTGTTTTGCATTTGCCTTGACACCACAACAGGTGCAGCAGATCAGCAGCT
CCATGGATATTTCTGGGACCAAATGTGACTTCACAGTGCAGGTCCAATTA
AGGTTTTGTTTATCAGAAACCAGTTGTCCACAAGAAGATCACTTCCCACC
CAACCTTTGTGTAAAAGTGAATACAAAACCTTGCAGCCTTCCAGGTTACC
TTCCACCTACTAAAAACGGTGTGGAACCAAAGCGACCTagtcgtcctATT
AATATCACCTCACTTGTCCGATTGTCCACGACAGTACCAAATACCATTGT
TGTTTCTTGGACTGCAGAAATTGGAAGAACCTATTCCATGGCAGTATATC
TTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGAGCAAAG
GGAATAagaaacccaGATCATTCTAGAGCTTTAATTAAAGAGAAGTTAAC
TGCAGATCCAGATAGTGAGATAGCTACTACCAGCCTACGGGTTTCGCTGC
TGTGTCCACTTGGGAAAATGCGACTGACAATCCCCTGTCGGGCACTTACC
TGCTCCCACCTTCAGTGTTTTGATGCAACTCTTTACATTCAAATGAATGA
GAAAAAACCAACATGGGTTTGTCCTGTCTGTGATAAGAAGGCCCCATATG
AACACCTTATTATTGACGGGTTGTTTATGGAAATTCTAAAGTACTGCACA
GACTGTGACGAGATACAGTTTAAGGAGGATGGCTCGTGGGCTCCAATGAG
GTCAAAGAAGGAGGTTCAAGAAGTCACTGCCTCCTACAATGGAGTTGATG
GTTGCTTGAGCTCCACATTGGAGCATCAGGTAGCGTCCCACAACCAGTCC
TCAAATAAAAACAAGAAATCGTCAGATGAAGAGGAGGAAGAACCCCCTGC
CAAGAGGACCTGTCCTTCCCTGTCTCCTACGTCACCACTAAGTAATAAAG
GCATTTTAAGTCTTCCTCATCAAGCCTCGCCTGTGTCCCGCACCCCAAGC
CTTCCTGCTGTAGATACAAGCTACATCAACACCTCCCTCATCCAGGACTA
CAGGCACCCCTTCCACATGACGCCTATGCCTTATGACTTACAAGGATTAG
ATTTCTTTCCTTTCTTATCAGGAGACAATCAGCATTACAACACCTCCCTG
CTAGCCGCAGCTGCAGCGGCGGTCTCAGATGACCAGGACCTCCTGCACTC
CTCCCGGTTTTTCCCGTATACCTCCTCGCAGATGTTTCTCGACCAGCTAA
GTGCAGGAGGGAGCACATCTCTGCCAGCCACCAACGGAAGCAGTAGCGGC
AGCAACAGCAGCCTTGTGTCTTCCAACAGTCTGAGAGAGAGCCATGGCCA
TGGTGTGGCCAGCAGGAGCAGCGCAGACACAGCGTCCATCTTTGGCATCA
TACCAGACATTATCTCATTGGACTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSMADSAELKQ
MVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKE
LYRRRFPQKIMTPADLSIPNVHSSPMPPTLSPSTIPQLTYDGHPASSPLL
PVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASD
NSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSC
PQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLS
TTVPNTIVVSWTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSR
ALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDA
TLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKE
DGSWAPMRSKKEVQEVTASYNGVDGCLSSTLEHQVASHNQSSNKNKKSSD
EEEEEPPAKRTCPSLSPTSPLSNKGILSLPHQASPVSRTPSLPAVDTSYI
NTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVS
DDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPATNGSSSGSNSSLVSSN
SLRESHGHGVASRSSADTASIFGIIPDIISLD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24569
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutations at S247 R248 P249 (agc cga cca → agt cgt cct ) and R303 N304 P305 (agg aat ccg → aga aac cca) plus SIM mutation (delta V457-S466)