cDNA Clone PIAS4

• Plasmid: pcDNA5-FRT/TO-EYFP-PIAS4 (siRNA resistant) deletion SAP
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: EYFP-PIAS4 (siRNA resistant) delta V6-R65
• Genbank: BC029874 NM_015897.2
• Tag: YFP
• Species: Human

Sequence of Insert

AAGATGGCGGCGGAGCTGTACGCCAAGAAGAACTCGGAGCCTGCCCCACA
GCCGCACCGGCCCCTGGACCCCCTGACCATGCACTCCACCTACGACCGGG
CCGGCGCTGTGCCCAGGACTCCGCTGGCAGGCCCCAATATTGACTACCCC
GTGCTCTACGGAAAGTACTTAAACGGACTGGGACGGTTGCCCGCCAAGAC
CCTCAAGCCAGAAGTCCGCCTGGTGAAGCTGCCGTTCTTTAATATGCTGG
ATGAGCTGCTGAAGCCCACCGAAttggttcctCAGAACAACGAGAAGCTT
CAGGAGAGCCCGTGCATCTTCGCATTGACGCCAAGACAGGTGGAGTTGAT
CCGGAACTCCAGGGAACTGCAGCCCGGAGTTAAAGCCGTGCAGGTCGTCC
TGAGAATCTGTTACTCAGACACCAGCTGCCCTCAGGAGGACCAGTACCCG
CCCAACATCGCTGTGAAGGTCAACCACAGCTACTGCTCCGTCCCGGGCTA
CTACCCCTCCAATAAGCCCGGGGTGGAGCCCAAGAGGCCGTGCCGCCCCA
TCAACCTCACTCACCTCATGTACCTGTCCTCGGCCACCAACCGCATCACT
GTCACCTGGGGGAACtatggtaaaAGCTACTCGGTGGCCCTGTACCTGGT
GCGGCAGCTGACCTCATCGGAGCTGCTGCAGAGGCTGAAGACCATTGGGG
TAAAGCACCCGGAGCTGTGCAAGGCACTGGTCAAGGAGAAGCTGCGCCTT
GATCCTGACAGCGAGATCGCCACCACCGGTGTGCGGGTGTCCCTCATCTG
TCCGCTGGTGAAGATGCGGCTCTCCGTGCCCTGCCGGGCAGAGACCTGCG
CCCACCTGCAGTGCTTCGACGCCGTCTTCTACCTGCAGATGAACGAGAAG
AAGCCCACCTGGATGTGCCCCGTGTGCGACAAGCCAGCCCCCTACGACCA
GCTCATCATCGACGGGCTCCTCTCGAAGATCCTGAGCGAGTGTGAGGACG
CCGACGAGATCGAGTACCTGGTGGACGGCTCGTGGTGCCCGATCCGCGCC
GAAAAGGAGCGCAGCTGCAGCCCGCAGGGCGCCATCCTCGTGCTGGGCCC
CTCGGACGCCAATGGGCTCCTGCCCGCCCCCAGCGTCAACGGGAGCGGTG
CCCTGGGCAGCACGGGTGGCGGCGGCCCGGTGGGCAGCATGGAGAATGGG
AAGCCGGGCGCCGATGTGGTGGACCTCACGCTGGACAGCTCATCGTCCTC
GGAGGATGAGGAGGAGGAGGAAGAGGAGGAGGAAGACGAGGACGAAGAGG
GGCCCCGGCCCAAGCGCCGCTGCCCCTTCCAGAAGGGCCTGGTGCCGGCC
TGCTGACCCCGGCCGCACACTCGACgcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSKMAAELYAK
KNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDYPVLYGKYLNG
LGRLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEKLQESPCIFAL
TPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPPNIAVKVNH
SYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKS
YSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATT
GVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVC
DKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIRAEKERSCSPQ
GAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMENGKPGADVVDL
TLDSSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVPAC*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24570
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutations at L151 V152 P153 (tta gtc cca → ttg gtt cct ) and Y265 G266 K267 (tac ggc aag → tat ggt aaa) plus SAP mutation (delta V6-R65). Clone has an additional N-terminal lysine upstream from PIAS4 initiation. ORF stop located approx 20bp upstream of 3' Not1 cloning site.