cDNA Clone PIAS4

• Plasmid: pcDNA5-FRT/TO-EYFP-PIAS4 (siRNA resistant) deletion SIM
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: EYFP-PIAS4 (siRNA resistant) delta A463-S472
• Genbank: BC029874 NM_015897.2
• Tag: YFP
• Species: Human

Sequence of Insert

ggatccAAGATGGCGGCGGAGCTGGTGGAGGCCAAAAACATGGTGATGAG
TTTTCGAGTCTCCGACCTTCAGATGCTCCTGGGTTTCGTGGGCCGGAGTA
AGAGTGGACTGAAGCACGAGCTCGTCACCAGGGCCCTCCAGCTGGTGCAG
TTTGACTGTAGCCCTGAGCTGTTCAAGAAGATCAAGGAGCTGTACGAGAC
CCGCTACGCCAAGAAGAACTCGGAGCCTGCCCCACAGCCGCACCGGCCCC
TGGACCCCCTGACCATGCACTCCACCTACGACCGGGCCGGCGCTGTGCCC
AGGACTCCGCTGGCAGGCCCCAATATTGACTACCCCGTGCTCTACGGAAA
GTACTTAAACGGACTGGGACGGTTGCCCGCCAAGACCCTCAAGCCAGAAG
TCCGCCTGGTGAAGCTGCCGTTCTTTAATATGCTGGATGAGCTGCTGAAG
CCCACCGAAttggttcctCAGAACAACGAGAAGCTTCAGGAGAGCCCGTG
CATCTTCGCATTGACGCCAAGACAGGTGGAGTTGATCCGGAACTCCAGGG
AACTGCAGCCCGGAGTTAAAGCCGTGCAGGTCGTCCTGAGAATCTGTTAC
TCAGACACCAGCTGCCCTCAGGAGGACCAGTACCCGCCCAACATCGCTGT
GAAGGTCAACCACAGCTACTGCTCCGTCCCGGGCTACTACCCCTCCAATA
AGCCCGGGGTGGAGCCCAAGAGGCCGTGCCGCCCCATCAACCTCACTCAC
CTCATGTACCTGTCCTCGGCCACCAACCGCATCACTGTCACCTGGGGGAA
CtatggtaaaAGCTACTCGGTGGCCCTGTACCTGGTGCGGCAGCTGACCT
CATCGGAGCTGCTGCAGAGGCTGAAGACCATTGGGGTAAAGCACCCGGAG
CTGTGCAAGGCACTGGTCAAGGAGAAGCTGCGCCTTGATCCTGACAGCGA
GATCGCCACCACCGGTGTGCGGGTGTCCCTCATCTGTCCGCTGGTGAAGA
TGCGGCTCTCCGTGCCCTGCCGGGCAGAGACCTGCGCCCACCTGCAGTGC
TTCGACGCCGTCTTCTACCTGCAGATGAACGAGAAGAAGCCCACCTGGAT
GTGCCCCGTGTGCGACAAGCCAGCCCCCTACGACCAGCTCATCATCGACG
GGCTCCTCTCGAAGATCCTGAGCGAGTGTGAGGACGCCGACGAGATCGAG
TACCTGGTGGACGGCTCGTGGTGCCCGATCCGCGCCGAAAAGGAGCGCAG
CTGCAGCCCGCAGGGCGCCATCCTCGTGCTGGGCCCCTCGGACGCCAATG
GGCTCCTGCCCGCCCCCAGCGTCAACGGGAGCGGTGCCCTGGGCAGCACG
GGTGGCGGCGGCCCGGTGGGCAGCATGGAGAATGGGAAGCCGGGCTCATC
GTCCTCGGAGGATGAGGAGGAGGAGGAAGAGGAGGAGGAAGACGAGGACG
AAGAGGGGCCCCGGCCCAAGCGCCGCTGCCCCTTCCAGAAGGGCCTGGTG
CCGGCCTGCTGACCCCGGCCGCACACTCGACgcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSKMAAELVEA
KNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKI
KELYETRYAKKNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDY
PVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEK
LQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQY
PPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRI
TVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLR
LDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNE
KKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIR
AEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMEN
GKPGSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVPAC*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24571
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutations at L151 V152 P153 (tta gtc cca → ttg gtt cct ) and Y265 G266 K267 (tac ggc aag → tat ggt aaa) plus SIM mutation (delta A463-S472). clone has an additional N-terminal lysine upstream from PIAS4 initiation. ORF stop located approx 20bp upstream of 3' Not1 cloning site.