cDNA Clone FAM83G

• Synonyms: FLJ41564
• Plasmid: pBABED H TIGHT FAM83G
• Parent Plasmid: pBabeD H Tight
• Expressed: FAM83G
• Genbank: NM_001039999.2
• Species: Human

Sequence of Insert

GGATCCATGGCCTTCTCTCAGGTGCAGTGTCTGGACGACAACCATGTGAACTGGCGCTCCAGCGAGTCCAAGCCTGAGTTCTTCTACAGCGAGGAGCAGCGGCTGGCGCTGGAGGCCCTGGTGGCCCGCGGCCGGGACGCCTTCTACGAGGTGCTCAAGCGGGAGAACATCCGAGACTTCCTCTCGGAGCTGGAGCTCAAGCGCATCCTGGAGACCATCGAGGTGTACGACCCGGGCTCTGAGGACCCTCGGGGCACGGGCCCCTCTCAGGGGCCCGAGGACAATGGGGTCGGCGACGGCGAGGAAGCCAGCGGGGCGGATGGGGTCCCCATCGAGGCCGAGCCGCTGCCCTCCCTGGAGTACTGGCCCCAGAAGTCGGACCGCTCCATCCCGCAGCTGGACCTGGGCTGGCCCGACACCATCGCCTACCGCGGCGTGACCCGGGCTAGCGTCTACATGCAGCCCCCCATAGACGGGCAGGCCCACATCAAAGAGGTGGTGCGGAAGATGATCAGCCAGGCACAGAAGGTGATAGCTGTGGTCATGGACATGTTCACCGACGTGGACATCTTCAAGGACCTGCTGGACGCCGGCTTCAAGAGGAAAGTGGCCGTGTACATCATCGTGGATGAGAGTAACGTCAAGTACTTCCTGCACATGTGTGAGCGGGCCTGCATGCACCTGGGGCACCTCAAGAATCTCAGAGTGCGGAGCAGCGGGGGAACTGAGTTCTTCACGCGGTCGGCAACCAAGTTCAAGGGTGCCCTGGCCCAGAAGTTCATGTTTGTGGATGGAGACCGGGCTGTGTGCGGCTCCTACAGCTTCACGTGGTCGGCCGCGCGGACGGACCGGAATGTGATCTCTGTGCTGTCTGGCCAGGTGGTGGAGATGTTTGACCGGCAGTTCCAGGAGCTGTACCTCATGTCACACAGTGTGAGCCTCAAGGGCATCCCTATGGAGAAGGAACCGGAGCCGGAGCCTATTGTGCTGCCCTCTGTGGTCCCCCTGGTGCCCGCGGGCACTGTGGCCAAGAAGCTCGTCAACCCCAAGTACGCACTTGTCAAGGCCAAGAGCGTCGACGAGATTGCCAAGATCTCCTCTGAGAAGCAGGAGGCCAAGAAGCCCCTGGGGCTGAAAGGCCCAGCGCTGGCTGAGCATCCAGGGGAACTCCCCGAGCTGCTGCCACCCATCCACCCAGGACTGCTTCACCTGGAGAGGGCCAACATGTTTGAGTACCTGCCCACGTGGGTGGAGCCAGACCCGGAGCCTGGCAGCGACATCCTGGGCTACATCAATATCATCGACCCCAACATCTGGAACCCCCAGCCCAGCCAGATGAACCGCATCAAGATCCGTGACACCTCCCAGGCCAGCGCCCAGCACCAGCTGTGGAAGCAGAGCCAGGACAGCAGGCCCCGTCCAGAGCCTTGCCCTCCCCCAGAGCCCAGTGCCCCCCAGGACGGTGTCCCAGCTGAGAACGGCCTCCCCCAGGGGGACCCTGAGCCATTGCCCCCCGTGCCCAAGCCCCGGACAGTCCCTGTGGCAGATGTACTAGCCCGGGACAGCAGTGATATTGGCTGGGTCCTGGAGCTCCCCAAAGAGGAAGCTCCCCAGAATGGGACAGACCATAGGCTACCCAGGATGGCAGGCCCAGGCCACGCCCCACTCCAGCGGCAGCTATCTGTGACCCAGGATGACCCCGAGAGCCTCGGGGTGGGGCTCCCCAATGGGCTGGATGGGGTGGAAGAAGAAGATGATGACGACTACGTAACCCTCAGTGACCAGGACAGCCACTCAGGCAGCTCCGGCCGTGGCCCTGGCCCCCGACGGCCCTCAGTGGCTTCCTCTGTGTCAGAGGAGTACTTCGAGGTGAGAGAGCACTCAGTCCCTCTCCGGAGGCGCCACTCAGAGCAAGTGGCCAACGGGCCAACCCCACCACCGCGCCGGCAGCTGAGTGCCCCCCATATAACCCGAGGGACCTTTGTTGGACCCCAGGGTGGCTCCCCATGGGCCCAGAGTCGGGGAAGAGAAGAAGCAGATGCGTTGAAGAGGATGCAGGCCCAGCGCTCCACAGACAAGGAGGCACAGGGCCAGCAGTTTCATCATCACAGGGTCCCTGCCTCAGGGACTAGGGATAAAGACGGCTTCCCAGGACCCCCTAGGTACCGCTCTGCTGCTGACAGCGTCCAGAGCTCTACCAGAAACGCTGGCCCAGCCATGGCTGGCCCCCACCACTGGCAGGCCAAGGGAGGTCAGGTACCCCGCCTGCTTCCGGACCCCGGCAGCCCAAGACTGGCCCAAAATGCCCGCCCCATGACCGATGGCAGGGCCACCGAGGAGCATCCGAGTCCCTTCGGAATCCCATACTCCAAACTGTCTCAGTCGAAGCACCTAAAGGCCAGGACGGGCGGTAGCCAGTGGGCCTCATCGGATTCTAAACGGAGGGCTCAAGCCCCCCGGGACCGCAAAGACCCCTAGGCGGCCGC

Amino Acid Sequence

MAFSQVQCLDDNHVNWRSSESKPEFFYSEEQRLALEALVARGRDAFYE
VLKRENIRDFLSELELKRILETIEVYDPGSEDPRGTGPSQGPEDNGVGDG
EEASGADGVPIEAEPLPSLEYWPQKSDRSIPQLDLGWPDTIAYRGVTRAS
VYMQPPIDGQAHIKEVVRKMISQAQKVIAVVMDMFTDVDIFKDLLDAGFK
RKVAVYIIVDESNVKYFLHMCERACMHLGHLKNLRVRSSGGTEFFTRSAT
KFKGALAQKFMFVDGDRAVCGSYSFTWSAARTDRNVISVLSGQVVEMFDR
QFQELYLMSHSVSLKGIPMEKEPEPEPIVLPSVVPLVPAGTVAKKLVNPK
YALVKAKSVDEIAKISSEKQEAKKPLGLKGPALAEHPGELPELLPPIHPG
LLHLERANMFEYLPTWVEPDPEPGSDILGYINIIDPNIWNPQPSQMNRIK
IRDTSQASAQHQLWKQSQDSRPRPEPCPPPEPSAPQDGVPAENGLPQGDP
EPLPPVPKPRTVPVADVLARDSSDIGWVLELPKEEAPQNGTDHRLPRMAG
PGHAPLQRQLSVTQDDPESLGVGLPNGLDGVEEEDDDDYVTLSDQDSHSG
SSGRGPGPRRPSVASSVSEEYFEVREHSVPLRRRHSEQVANGPTPPPRRQ
LSAPHITRGTFVGPQGGSPWAQSRGREEADALKRMQAQRSTDKEAQGQQF
HHHRVPASGTRDKDGFPGPPRYRSAADSVQSSTRNAGPAMAGPHHWQAKG
GQVPRLLPDPGSPRLAQNARPMTDGRATEEHPSPFGIPYSKLSQSKHLKA
RTGGSQWASSDSKRRAQAPRDRKDP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48440
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Retroviral vector. FAM83G under control of TRE3GS promoter, induce with doxycycline. Silent T>C 2271 bp