cDNA Clone SPAK (mouse)

• Synonyms: Stk39, AW227544, AW556857, DCHT, RF005, Rnl5
• Plasmid: pCMV5D FLAG mSPAK Opt L502A
• Parent Plasmid: pCMV5D FLAG
• Expressed: FLAG-mSPAK Opt L502A
• Genbank: NM_016866.2 (this sequence is optimised however)
• Tag: FLAG
• Species: Mouse

Sequence of Insert

GGATCCATGGCCGAGCCTAGCGGATCTCCCGTGCATGTGCAGCTGTCTCAGCAGGCCGCTCCTGTGACAGCCGCTGCTGCTACAGCTCCTGCCGCCGCTACATCTGCTCCAGCACCAGCTCCAGCCCCTGCTCCTGCTGCTTCTGCCGCCCCTGCACCCGCACCAGCTGCCGCTCCCGCTCCAGCTCCCGCAGCTCAGGCTGTGGGATGGCCCATCTGCAGAGATGCCTACGAGCTGCAGGAAGTGATCGGCAGCGGAGCCACAGCAGTGGTGCAGGCTGCCCTGTGCAAGCCCAGACAGGAACGGGTGGCCATCAAGCGGATCAACCTGGAAAAGTGCCAGACCAGCATGGACGAGCTGCTGAAAGAAATCCAGGCCATGAGCCAGTGCAGCCACCCCAACGTCGTGACCTACTACACCAGCTTCGTCGTGAAGGATGAGCTGTGGCTCGTGATGAAGCTGCTGAGCGGCGGCTCTATGCTGGACATCATCAAGTACATCGTGAACCGGGGCGAGCACAAGAACGGCGTGCTGGAAGAGGCCATCATTGCCACCATCCTGAAAGAGGTGCTGGAAGGCCTGGACTACCTGCACCGGAACGGCCAGATCCACAGGGATCTGAAGGCCGGCAACATCCTGCTGGGCGAGGATGGCTCTGTGCAGATCGCCGATTTTGGCGTGTCCGCCTTCCTGGCCACTGGCGGCGACGTGACCAGAAACAAAGTGCGCAAGACCTTCGTGGGCACCCCCTGCTGGATGGCCCCCGAAGTGATGGAACAAGTGCGGGGCTACGACTTCAAGGCCGACATGTGGTCCTTCGGCATCACCGCCATCGAGCTGGCTACAGGCGCCGCTCCCTACCACAAGTACCCCCCCATGAAGGTGCTGATGCTGACCCTGCAGAACGACCCCCCCACCCTGGAAACCGGCGTGGAGGACAAAGAAATGATGAAGAAGTACGGCAAGAGCTTCCGCAAGCTGCTGTCCCTGTGCCTGCAGAAAGATCCCAGCAAGAGGCCCACAGCCGCCGAACTGCTGAAGTGCAAGTTCTTCCAGAAGGCCAAGAACCGCGAGTACCTGATCGAGAAACTGCTGACCCGGACCCCCGATATCGCCCAGCGGGCTAAGAAAGTGCGGAGAGTGCCTGGCAGCTCCGGACATCTGCACAAGACCGAGGACGGCGACTGGGAGTGGAGCGACGACGAGATGGACGAGAAGTCCGAGGAAGGCAAGGCCGCTGCCAGCCAGGAAAAGTCTCGGCGCGTGAAAGAGGAAAACAGCGAGATCAGCGTGAACGCTGGCGGCATCCCCGAGCAGATCCAGAGCCTGAGCGTGCACGATAGCCAGGCCCAGCCCAACGCCAACGAGGACTACAGAGAAGGCCCCTGCGCCGTGAACCTGGTGCTGAGACTGCGGAACAGCCGGAAAGAGCTGAACGACATCAGATTCGAGTTCACCCCCGGCAGAGACACCGCTGATGGGGTGTCCCAGGAACTGTTCAGCGCCGGAGCGGTGGACGGCCACGATGTCGTGATTGTGGCCGCCAATCTGCAGAAAATCGTGGACGACCCCAAGGCCCTGAAAACCCTGACCTTCAAGCTGGCCTCCGGCTGCGACGGCTCCGAGATCCCTGATGAAGTGAAGCTGATCGGCTTCGCCCAGCTGAGCGTGTCCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMAEPSGSPVHVQLSQQAAPVTAAAATAPAAATSAPAPAP
APAPAASAAPAPAPAAAPAPAPAAQAVGWPICRDAYELQEVIGSGATAVV
QAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTS
FVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVL
EGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNK
VRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYP
PMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPT
AAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKT
EDGDWEWSDDEMDEKSEEGKAAASQEKSRRVKEENSEISVNAGGIPEQIQ
SLSVHDSQAQPNANEDYREGPCAVNLVLRLRNSRKELNDIRFEFTPGRDT
ADGVSQELFSAGAVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGS
EIPDEVKLIGFAQLSVS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48455
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: L502A. Optimised SPAK mouse sequence to minimise issues with propagation in bacteria.; ; All SPAK plasmids MUST be grown at 30 degrees C or less to prevent recombination.